Detecting exosomal PD-L1 secreted by cancer cells

Exosomes is the extracellular vesicles secreted by all cells. Evidences accumulated that exosomes implicate in various diseases including cancer metastasis and neurodegeneration diseases. PD-L1 is an immune checkpoint inhibitor that keep T cells from attacking other cells in the body. Many cancer cells overexpress PD-L1 to escape the immunological attack.

The exosomal PD-L1 has been isolated in multiple cancer cells. Recently, Chen et al. elucidated the regulation and immunosuppressive function of exosomal PD-L1 and published their result in Nature. They found that IFNγ treatment enhances the levels of PD-L1 in exosomes. In mouse model, exosomal PD-L1 inhibits CD8+ T cells and facilitates the cancer progression. Moreover, the authors examined the exosomel PD-L1 levels in melanoma patients during anti-PD-1 therapy and found that the pretreatment level of exosomal PD-L1 was higher in non-responders which was associated with poor prognosis. Their study provides a rationale for the application of exosomal PD-L1 as a predictor for anti-PD-1 therapy.

arigo offers excellent ELISA kits to detect exosomal PD-L1 level. In addition, more quality antibodies and ELISA kits are available for facilitating related studies.


Detecting exosomal PD-L1

Human PD-L1 ELISA Kit (ARG81929) Sensitivity: 31.25 pg/ml  Samples: Serum, Plasma, Cell culture sup.

Mouse PD-L1 ELISA Kit (ARG81930) Sensitivity: 15.6 pg/ml  Samples: Serum, Plasma, Cell culture sup.

 

Related ELISA Kits

Human IFN gamma ELISA Kit (ARG80117) Sensitivity: 8 pg/ml	Human Galectin 3 ELISA Kit (ARG81257) Sensitivity: 31 pg/ml	Human Galectin 9 ELISA Kit (ARG81503) Sensitivity: 46.9 pg/ml
Mouse IFN gamma ELISA Kit (ARG80205) Sensitivity: 3.9 pg/ml	Mouse Galectin 3 ELISA Kit (ARG81736) Sensitivity: 31.25 pg/ml	Human PD-1 ELISA Kit (ARG81360) Sensitivity: 0.44 pg/ml

 

Exosome markers

CD9 antibody [MEM-61] (FITC) (ARG62944)	CD63 antibody [NKI/C3] (ARG55941)	CD81 antibody [2F7] (PE) (ARG21039)
TSG101 antibody (ARG55805)	Alix antibody [3A9] (ARG22563)	Hrs antibody (ARG63936)

 

Product	Unconjugated	APC	Biotin	FITC	PE
CD9 antibody [MM2/57]	ARG21247			ARG21248	ARG21249
CD9 antibody [MEM-61]	ARG62943	ARG53912	ARG62942	ARG62944	ARG53913
CD63 antibody [MEM-259]	ARG62912	ARG53886	ARG62911	ARG62913	ARG53887
CD81 antibody [M38]	ARG62934			ARG62935	ARG53906
CD82 antibody [C33]	ARG54184	ARG65635	ARG65632	ARG65633	ARG65634

Reference: Chen et al., (2018) Nature. 560(7718):382-386.