APOPTOSIS

Cell Biology Apoptosis is a process of programmed cell death that occurs in multicellular organism. Intracellular or extracellular death signals kick-start apoptosis signaling by releasing cytochrome-c from mitochondria through BAX. The released cytochrome-c binds to APAF-1 and cleave caspase 9. Finally, caspase-3 and PARP will be cleaved and the cells officially go into apoptosis and cell death occurs.

 

 

TOP 3  APOPTOSIS must-haves

Apoptosis Marker
Antibody Duo (Bcl2, Bax)
ARG30268

A: MCF7, B: HCT116, C: U87-MG, D: HepG2, E: Jurkat

BAX/BCL2 expression ratio has been identified as an import index to figure out the fate of the cell undergoing apoptosis or anti-apoptosis in variety studies, including cancer study, therapeutic agent searching and therapeutic response evaluation.

Shared reactivity: Human, Mouse Rat
Shared application: WB, IHC-P

 

 

 

Caspase 9 Antibody
ARG54155

A: HeLa untreated, B: HeLa treated with Staurosporine 1μM (4h)

When a cell receive intra- or extra-cellular death signal, Cytochrome C is released from mitochondria to cytoplasm and trigger Procaspase 9 activation. Detection of the cleaved Caspase 9 represents early indications that a cell is undergoing apoptosis.

Reactivity: Human
Application: WB
Specialty: Recognizing both Caspase 9 precursor and cleaved Caspase 9 protein

 

 

PARP (cleaved) Antibody
ARG20041

A: HeLa untreated, B: HeLa treated with Staurosporine 1μM (4h)

Cleavage of PARP is a pre-requisite for cells who undergo final stage of cell death. The use of anti-cleaved PARP antibody adds a more specific weapon to the arsenal of techniques for confirming apoptosis in tissue specimens.

Reactivity: Human
Application: WB, IHC
Specialty: Recognizing only cleaved PARP p85 protein