ARG54184
anti-CD82 antibody [C33]
anti-CD82 antibody [C33] for Flow cytometry,Functional study,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Human
Cancer antibody; Gene Regulation antibody; Immune System antibody
Overview
| Product Description | Mouse Monoclonal antibody [C33] recognizes CD82 |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | FACS, FuncSt, ICC/IF, IHC-P, IP, WB |
| Specificity | The clone C33 reacts with CD82, a widely expressed cell surface protein of the tetraspanin family. CD82 is also found in endosome/lysosome compartments. |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | C33 |
| Isotype | IgG2a |
| Target Name | CD82 |
| Antigen Species | Human |
| Immunogen | C91/PL (human HTLV-1+ T cell line) |
| Conjugation | Un-conjugated |
| Alternate Names | Tetraspanin-27; C33 antigen; R2; Tspan-27; TSPAN27; KAI1; CD antigen CD82; CD82 antigen; ST6; 4F9; Suppressor of tumorigenicity 6 protein; SAR2; IA4; Metastasis suppressor Kangai-1; Inducible membrane protein R2; GR15; C33 |
Application Instructions
| Application Suggestion |
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| Application Note | Functional studies: In human MOLT-4 T-cell line the antibody C33 inhibits syncytium formation induced by coculture with human T-cell leukemia virus type 1 (HTLV-1)-positive human T-cell lines. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from cell culture supernatant by protein-A affinity chromatography. |
| Purity | > 95% (by SDS-PAGE) |
| Buffer | PBS (pH 7.4) and 15 mM Sodium azide |
| Preservative | 15 mM Sodium azide |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | CD82 |
| Gene Full Name | CD82 molecule |
| Background | CD82 (KAI1), a member of the tetraspanin family, forms complexes with other tetraspanin proteins, integrins, coreceptors, MHC class I and II molecules. These complexes influence adhesion, morphology, activation, proliferation and differentiation of B, T and other cells. CD82 regulates cytoskeleton rearrangement and may participate in the turnover of the tetraspanin complex members. Besides in the plasma membrane, CD82 is localized also in endosome/lysosome compartments. Tumour-suppressive roles of CD82 have been demonstrated. |
| Function | Associates with CD4 or CD8 and delivers costimulatory signals for the TCR/CD3 pathway. [UniProt] |
| Highlight | Related products: CD82 antibodies; Anti-Mouse IgG secondary antibodies; Related news: Detecting exosomal PD-L1 secreted by cancer cells New antibodies for exosome isolation |
| Research Area | Cancer antibody; Gene Regulation antibody; Immune System antibody |
| Calculated MW | 30 kDa |
Images (3) Click the Picture to Zoom In
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ARG54184 anti-CD82 antibody [C33] IHC-P image
Immunohistochemistry: Paraffin-embedded Human tonsil tissue stained with ARG54184 anti-CD82 antibody [C33].
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ARG54184 anti-CD82 antibody [C33] FACS image
Flow Cytometry: Separation of human CD82 positive lymphocytes (red-filled) from CD82 negative lymphocytes (black-dashed). Human peripheral whole blood stained with ARG54184 anti-CD82 antibody [C33] at 1 µg/ml dilution, followed by APC-conjugated Goat anti-Mouse antibody.
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ARG54184 anti-CD82 antibody [C33] FACS image
Flow Cytometry: Human peripheral blood stained with ARG54184 anti-CD82 antibody [C33] at 1 µg/ml dilution, followed by APC-conjugated Goat anti-Mouse antibody.
