ARG81736
Mouse Galectin 3 ELISA Kit
Mouse Galectin 3 ELISA Kit for ELISA and Mouse
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG81736-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG81736-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG81736-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG81736-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81736-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81736-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81736-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81736-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG81736-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG81736-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG81736-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG81736 Mouse Galectin 3 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Mouse Galectin 3 in serum, plasma (heparin, EDTA) and cell culture supernatants. |
|---|---|
| Tested Reactivity | Ms |
| Tested Application | ELISA |
| Specificity | There is no detectable cross-reactivity with other relevant proteins. |
| Target Name | Galectin 3 |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 31.25 pg/ml |
| Sample Type | Serum, plasma (heparin, EDTA) and cell culture supernatants. |
| Standard Range | 62.5 - 4000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 7.6%; Inter-Assay CV: 8.9% |
| Alternate Names | Laminin-binding protein; Gal-3; L-31; GALBP; Galactoside-binding protein; MAC2; GAL3; GALIG; Mac-2 antigen; CBP 35; Galectin-3; CBP35; Galactose-specific lectin 3; IgE-binding protein; L31; 35 kDa lectin; Carbohydrate-binding protein 35; Lectin L-29 |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Gene Symbol | LGALS3 |
|---|---|
| Gene Full Name | lectin, galactoside-binding, soluble, 3 |
| Background | This gene encodes a member of the galectin family of carbohydrate binding proteins. Members of this protein family have an affinity for beta-galactosides. The encoded protein is characterized by an N-terminal proline-rich tandem repeat domain and a single C-terminal carbohydrate recognition domain. This protein can self-associate through the N-terminal domain allowing it to bind to multivalent saccharide ligands. This protein localizes to the extracellular matrix, the cytoplasm and the nucleus. This protein plays a role in numerous cellular functions including apoptosis, innate immunity, cell adhesion and T-cell regulation. The protein exhibits antimicrobial activity against bacteria and fungi. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Oct 2014] |
| Function | Galactose-specific lectin which binds IgE. May mediate with the alpha-3, beta-1 integrin the stimulation by CSPG4 of endothelial cells migration. Together with DMBT1, required for terminal differentiation of columnar epithelial cells during early embryogenesis (By similarity). In the nucleus: acts as a pre-mRNA splicing factor. Involved in acute inflammatory responses including neutrophil activation and adhesion, chemoattraction of monocytes macrophages, opsonization of apoptotic neutrophils, and activation of mast cells. [UniProt] |
| Highlight | Related products: Galectin 3 antibodies; Galectin 3 ELISA Kits; Galectin 3 recombinant proteins; Related news: Detecting exosomal PD-L1 secreted by cancer cells New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
Images (1) Click the Picture to Zoom In
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ARG81736 Mouse Galectin 3 ELISA Kit standard curve image
ARG81736 Mouse Galectin 3 ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG81736 Mouse Galectin 3 ELISA Kit User's manual |
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