NFkB Signaling Pathway

Immunology Nuclear factor kappa B (NFkB) is a protein complex that controls large number of genes responsible for the regulation of apoptosis, inflammation, tumorigenesis and a variety of immune responses. NF-kB is sensitive and responsive to stimuli such as cytokines, stress, UV, low oxygen, bacterial or viral infections.

 

 

TOP 3  NFKB SIGNALING must-haves

p38 phospho T180/Y182 Antibody
ARG51850

A: HeLa (untreated), B: HeLa + TNF-alpha (10 min), C: HeLa + TNF-alpha (20 min), D: HeLa + TNF-alpha (30 min)

As a downstream signaling molecule of NFkB, p38 MAPK is activated by a variety of cellular stresses including osmotic shock, inflammatory cytokines, lipopolysaccharide (LPS), UV light, and growth factors. MKK3, MKK6, and SEK activate p38 MAPK by phosphorylation at Thr180 and Tyr182.

Reactivity: Human, Mouse, Rat
Application: WB

 

 

NFkB p65 Antibody
ARG55798

NF-kappa-B is a ubiquitous transcription factor involved in several biological processes. It is held in the cytoplasm in an inactive state by specific inhibitors. Upon degradation of the inhibitor, NF-kappa-B moves to the nucleus and activates transcription of specific genes.

Reactivity: Human, Mouse, Rat
Application: WB, IHC-P, IF/ICC

 

 

I kappa B alpha Antibody
ARG54710

Upon NFkB pathway activation, IκB proteins are phosphorylated by IKKα and IKKβ, leading to the degradation of IκB proteins and nuclear translocation of NF-κB proteins. Hence, down-regulation of IkB alpha is often associated with the activation of NF-kB downstream signaling pathway.

Reactivity: Human, Mouse, Rat
Application: WB