ARG62934
anti-CD81 antibody [M38]
anti-CD81 antibody [M38] for Flow cytometry,Functional study,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Human,Cat,Rabbit
Immune System antibody; Microbiology and Infectious Disease antibody
Overview
| Product Description | Mouse Monoclonal antibody [M38] recognizes CD81 |
|---|---|
| Tested Reactivity | Hu, Cat, Rb |
| Tested Application | FACS, FuncSt, ICC/IF, IHC-P, IP, WB |
| Specificity | The clone M38 reacts with CD81, a 25 kDa member of the tetraspanin family, expressed on majority of cells. |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | M38 |
| Isotype | IgG1 |
| Target Name | CD81 |
| Antigen Species | Human |
| Immunogen | MOLT-4 (human T-ALL cell line) |
| Conjugation | Un-conjugated |
| Alternate Names | CD antigen CD81; TAPA1; Tspan-28; S5.7; CD81 antigen; Target of the antiproliferative antibody 1; Tetraspanin-28; 26 kDa cell surface protein TAPA-1; CVID6; TSPAN28 |
Application Instructions
| Application Suggestion |
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| Application Note | Functional Application: In human MOLT-4 T-cell line the antibody M38 inhibits syncytium formation induced by co-culturing with human T-cell leukemia virus type 1 (HTLV-1)-positive human T-cell lines. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from hybridoma culture supernatant by protein-A affinity chromatography. |
| Purity | > 95% (by SDS-PAGE) |
| Buffer | PBS (pH 7.4) and 15 mM Sodium azide |
| Preservative | 15 mM Sodium azide |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | CD81 |
| Gene Full Name | CD81 molecule |
| Background | CD81 (TAPA-1), a member of the tetraspanin family, is expressed on virtually all nucleated cells, but above all on germinal center B cells. CD81 forms complexes with other tetraspanin proteins, integrins, coreceptors, MHC class I and II molecules, and influences adhesion, morphology, activation, proliferation and differentiation of B, T and other cells – e.g. in muscles CD81 promotes cell fusion and myotube maintenance. CD81 has been also identified as a receptor for the hepatitis C virus. |
| Function | May play an important role in the regulation of lymphoma cell growth. Interacts with a 16-kDa Leu-13 protein to form a complex possibly involved in signal transduction. May act as the viral receptor for HCV. [UniProt] |
| Highlight | Related products: CD81 antibodies; Anti-Mouse IgG secondary antibodies; Related news: Tools for studying Exosomes Detecting exosomal PD-L1 secreted by cancer cells New antibodies for exosome isolation |
| Research Area | Immune System antibody; Microbiology and Infectious Disease antibody |
| Calculated MW | 26 kDa |
| PTM | Not glycosylated. |
Images (6) Click the Picture to Zoom In
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ARG62934 anti-CD81 antibody [M38] ICC/IF image
Immunofluorescence: Huh7.5 cells were fixed with 3% PFA, permeabilized by 0.05% saponin and blocked with 0.5% BSA/PBS. Cells were stained with ARG62934 anti-CD81 antibody [M38] for 1 hour at RT.
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ARG62934 anti-CD81 antibody [M38] WB image
Western blot: Jurkat cell lysate stained with ARG62934 anti-CD81 antibody [M38] at 2 µg/ml dilution, under non-reducing condition.
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ARG62934 anti-CD81 antibody [M38] WB image
Western blot: K562 cell lysate stained with ARG62934 anti-CD81 antibody [M38] at 1:1000 dilution, under non-reducing condition.
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ARG62934 anti-CD81 antibody [M38] FACS image
Flow Cytometry: Human peripheral blood stained with ARG62934 anti-CD81 antibody [M38] at 4 µg/ml dilution, followed by APC-conjugated Goat anti-Mouse antibody.
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ARG62934 anti-CD81 antibody [M38] WB image
Western blot: Extracellular vesicles isolated from K562 cells. Sample was stained with ARG62934 anti-CD81 antibody [M38] at 1:1000 dilution, under non-reducing condition.
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ARG62934 anti-CD81 antibody [M38] FACS image
Flow Cytometry: Separation of human lymphocytes (red-filled) from neutrophil granulocytes (black-dashed). Human peripheral whole blood stained with ARG62934 anti-CD81 antibody [M38] at 4 µg/ml dilution, followed by APC-conjugated Goat anti-Mouse antibody.
Clone References
Selective enrichment of tetraspan proteins on the internal vesicles of multivesicular endosomes and on exosomes secreted by human B-lymphocytes.
C33 antigen and M38 antigen recognized by monoclonal antibodies inhibitory to syncytium formation by human T cell leukemia virus type 1 are both members of the transmembrane 4 superfamily and associate with each other and with CD4 or CD8 in T cells.
Identification of membrane antigen C33 recognized by monoclonal antibodies inhibitory to human T-cell leukemia virus type 1 (HTLV-1)-induced syncytium formation: altered glycosylation of C33 antigen in HTLV-1-positive T cells.
