ARG81360

Human CD279 / PD-1 ELISA Kit

Human CD279 / PD-1 ELISA Kit for ELISA and Human

publication_link Publication1

Overview

Product Description ARG81360 Human CD279 / PD-1 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human CD279 / PD-1 in serum, plasma and cell culture supernatant.
Tested Reactivity Hu
Tested Application ELISA
Target Name CD279 / PD-1
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm.
Sensitivity 0.44 pg/ml
Sample Type Serum, plasma and cell culture supernatant.
Standard Range 15.6 - 1000 pg/ml
Sample Volume 100 µl
Alternate Names hPD-l; CD279; PD-1; Protein PD-1; CD antigen CD279; PD1; hSLE1; SLEB2; Programmed cell death protein 1; hPD-1

Application Instructions

Assay Time ~ 3.5 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 5133 Human PDCD1

Swiss-port # Q15116 Human Programmed cell death protein 1

Gene Symbol PDCD1
Gene Full Name programmed cell death 1
Background CD279 / PD-1 is a cell surface membrane protein of the immunoglobulin superfamily. This protein is expressed in pro-B-cells and is thought to play a role in their differentiation. In mice, expression of this gene is induced in the thymus when anti-CD3 antibodies are injected and large numbers of thymocytes undergo apoptosis. Mice deficient for this gene bred on a BALB/c background developed dilated cardiomyopathy and died from congestive heart failure. These studies suggest that this gene product may also be important in T cell function and contribute to the prevention of autoimmune diseases. [provided by RefSeq, Jul 2008]
Function CD279 / PD-1 is an inhibitory receptor on antigen activated T-cells. It plays a critical role in induction and maintenance of immune tolerance to self (PubMed:21276005). Delivers inhibitory signals upon binding to ligands CD274/PDCD1L1 and CD273/PDCD1LG2 (PubMed:21276005). Following T-cell receptor (TCR) engagement, PDCD1 associates with CD3-TCR in the immunological synapse and directly inhibits T-cell activation. Suppresses T-cell activation through the recruitment of PTPN11/SHP-2: following ligand-binding, PDCD1 is phosphorylated within the ITSM motif, leading to the recruitment of the protein tyrosine phosphatase PTPN11/SHP-2 that mediates dephosphorylation of key TCR proximal signaling molecules, such as ZAP70, PRKCQ/PKCtheta and CD247/CD3zeta.

The PDCD1-mediated inhibitory pathway is exploited by tumors to attenuate anti-tumor immunity and escape destruction by the immune system, thereby facilitating tumor survival (PubMed:28951311). The interaction with CD274/PDCD1L1 inhibits cytotoxic T lymphocytes (CTLs) effector function (PubMed:28951311). The blockage of the PDCD1-mediated pathway results in the reversal of the exhausted T-cell phenotype and the normalization of the anti-tumor response, providing a rationale for cancer immunotherapy (PubMed:22658127, PubMed:25034862, PubMed:25399552). [UniProt]
Highlight Related products:
PD-1 antibodies; PD-1 ELISA Kits; PD-1 Duos / Panels;
Related news:
Detecting exosomal PD-L1 secreted by cancer cells
The best solution for PD-1/PD-L1 research
Examining CTL/NK-mediated cytotoxicity by ELISA
New ELISA data calculation tool:
Simplify the ELISA analysis by GainData

Images (2) Click the Picture to Zoom In

  • ARG81360 Human CD279 / PD-1 ELISA Kit standard curve image

    ARG81360 Human CD279 / PD-1 ELISA Kit results of a typical standard run with optical density reading at 450 nm. (using CALIBRATOR DILUENT I)

  • ARG81360 Human CD279 / PD-1 ELISA Kit standard curve image

    ARG81360 Human CD279 / PD-1 ELISA Kit results of a typical standard run with optical density reading at 450 nm. (using CALIBRATOR DILUENT II)

Specific References

The TOX-RAGE axis mediates inflammatory activation and lung injury in severe pulmonary infectious diseases

ELISA / Human / Plasma

Hyelim Kim et al.
Proc Natl Acad Sci U S A.,  (2024)

publication_link

 

hr_line
Title Download Link
ARG81360 Human CD279 / PD-1 ELISA Kit User's manual download icon Download