Immunosuppressive role of HMGB1 in TME
Immunosuppressive role of HMGB1 in TME
HMGB1 is a multifunctional protein with various roles in different cellular compartments. It exhibits a dual role in cancer cells; the intracellular HMGB1 acts as an anti-tumor protein stabilizing the genome, whereas the chronic released HMGB1 behaves as a pro-tumor protein with immunosuppressive functions.
In tumor microenvironment (TME), tumor cell-secreted HMGB1 inhibits the activities of T cells by inhibiting the maturation of dendritic cells (DCs) and promoting the activities of myeloid-derived suppressor cells (MDSCs) and Treg. HMGB1 also inhibits anti-tumor activity by binding to the immunosuppressive receptor TIM‐3. Moreover, HMGB1 interacts with RAGE and induces tumor cell migration, invasion and metastasis.
HMGB1 is a potential prognosis marker and a target for cancer therapy. arigo offers quality antibodies and ELISA kits to facilitate the research of the detailed mechanism underlying the immune suppression by HMGB1.
HMGB1 ELISA kits and antibodies |
| Human HMGB1 ELISA Kit (ARG81185) |
Mouse/Rat HMGB1 ELISA Kit (ARG81310) | HMGB1 ELISA Kit (ARG81351) |
| Reactivity: Hu Sensitivity: 0.3 ng/ml Sample: Plasma, cell culture sup. |
Reactivity: Ms, Rat Sensitivity: 0.3 ng/ml Sample: Plasma, cell culture sup., lysates |
Reactivity: Hu, Ms, Rat Sensitivity: 0.15 ng/ml Sample: Serum |
|
|
|
|
|
|
|
|
Antibodies for MDSCs |
|
|
| Cat. No. | Product | Clonality | Reactivity | Application |
| ARG65463 | CD11b antibody [ICRF44] | Mouse mAb | Hu, NHuPrm | FACS, FuncSt, ICC/IF, IHC-Fr, IP |
| ARG54260 | CD11b antibody [ICRF44] (PE) | Mouse mAb | Hu, NHuPrm | FACS, ICC/IF, IHC-Fr |
| ARG22000 | CD11b antibody [M1/70] | Rat mAb | Hu, Ms, Bb, Mk | BL, FACS, ICC/IF, IHC-Fr, IHC-P, IP |
| ARG65478 | CD11b antibody [M1/70] (APC) | Rat mAb | Hu, Ms, Bb, Mk | FACS |
| ARG20843 | CD11b antibody [3A33] (PE-Cy7) | Rat mAb | Ms | FACS, ICC/IF |
| ARG65560 | CD33 antibody [WM53] | Mouse mAb | Hu, NHuPrm | FACS, FuncSt, ICC/IF, IHC-Fr, IP, WB |
| ARG22104 | Ly6G/Ly6C antibody [RB6-8C5] | Rat mAb | Ms | Depletion, FACS, IHC-Fr, IHC-P, IP |
Antibodies for DCs |
| Cat. No. | Product | Clonality | Reactivity | Application |
| ARG21322 | CD11c antibody [3.9] | Mouse mAb | Hu, Mk | BL, FACS, IHC-Fr, IP |
| ARG23522 | CD11c antibody [N418] | Hamster mAb | Ms | FACS, ICC/IF, IHC-Fr, IP |
| ARG23079 | Thrombomodulin antibody [QBEND/40] | Mouse mAb | Hu, Rb | FACS, IHC-Fr, IHC-P |
Antibodies for TAMs |
| Cat. No. | Product | Clonality | Reactivity | Application |
| ARG55553 | F4/80 antibody [BM8] | Rat mAb | Hu, Ms | FACS, IHC-Fr, WB |
| ARG53010 | F4/80 antibody [SP115] | Rabbit mAb | Hu, Ms | IHC-Fr, IHC-P, WB |
| ARG22838 | CD163 antibody [EDHu-1] | Mouse mAb | Hu, Bo, Gpig, Pig, Mk, Sh | ELISA, FACS, ICC/IF, IHC-Fr, IHC-P, WB |
| ARG56138 | CD163 antibody | Rabbit pAb | Hu, Ms, Rat | FACS, IHC-P, WB |
| ARG55554 | CD206 antibody [15-2] | Mouse mAb | Hu, Ms | FACS, FuncSt, ICC/IF, IHC-Fr, IP, WB |
| ARG22456 | CD206 antibody [MR5D3] | Rat mAb | Ms | FACS, ICC/IF, IHC-Fr, IP |
| ARG22372 | CD206 antibody | Rabbit pAb | Hu, Ms, Rat, Pig | ICC/IF, IHC-P, WB |
Antibodies for Treg |
|
FoxP3 antibody [SP97]
|
| Cat. No. | Product | Clonality | Reactivity | Application |
| ARG21295 | CD4 antibody [RFT4] | Mouse mAb | Hu | FACS, ICC/IF, IHC-Fr, WB |
| ARG21979 | CD4 antibody [GK1.5] | Rat mAb | Ms | BL, Depletion, FACS, ICC/IF, IHC-Fr, IP |
| ARG65448 | CD4 antibody [GK1.5] (PE) | Rat mAb | Ms | FACS, ICC/IF, IHC-Fr |
| ARG65860 | CD4 antibody [SQab1714] | Rabbit mAb | Hu | FACS, IHC-P, IP, WB |
| ARG54202 | FoxP3 antibody [3G3] | Mouse mAb | Hu, Ms | FACS, WB |
| ARG53050 | FoxP3 antibody [SP97] | Rabbit mAb | Hu | IHC-P |
