ARG53050

anti-FOXP3 antibody [SP97]

anti-FOXP3 antibody [SP97] for IHC-Formalin-fixed paraffin-embedded sections and Human

Cell Biology and Cellular Response antibody; Gene Regulation antibody; Immune System antibody; Regulatory T cells Study antibody

Overview

Product Description Rabbit Monoclonal antibody [SP97] recognizes FOXP3
Tested Reactivity Hu
Tested Application IHC-P
Host Rabbit
Clonality Monoclonal
Clone SP97
Isotype IgG
Target Name FOXP3
Antigen Species Human
Immunogen Synthetic peptide corresponding to C-terminus of human FOXP3 protein.
Conjugation Un-conjugated
Alternate Names FOXP3; Forkhead Box P3; DIETER; XPID; AIID; PIDX; IPEX; JM2; Immune Dysregulation, Polyendocrinopathy, Enteropathy, X-Linked; Forkhead Box Protein P3; SCURFIN; Immunodeficiency, Polyendocrinopathy, Enteropathy, X-Linked; FOXP3delta7; Scurfin

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-P1:100
Application Note IHC-P: Antigen Retrieval: Boil tissue section in EDTA buffer, pH 8.0 for 10 min followed by cooling at room temperature for 20 min.
Incubation Time: 20 min at RT.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control Tonsil

Properties

Form Liquid
Purification Purified by protein A/G
Buffer PBS (pH 7.6), 1% BSA and <0.1% Sodium azide
Preservative <0.1% Sodium azide
Stabilizer 1% BSA
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 50943 Human FOXP3

Swiss-port # Q9BZS1 Human Forkhead box protein P3

Gene Symbol FOXP3
Gene Full Name forkhead box P3
Background The protein encoded by this gene is a member of the forkhead/winged-helix family of transcriptional regulators. Defects in this gene are the cause of immunodeficiency polyendocrinopathy, enteropathy, X-linked syndrome (IPEX), also known as X-linked autoimmunity-immunodeficiency syndrome. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]
Function Transcriptional regulator which is crucial for the development and inhibitory function of regulatory T-cells (Treg). [UniProt]

Plays an essential role in maintaining homeostasis of the immune system by allowing the acquisition of full suppressive function and stability of the Treg lineage, and by directly modulating the expansion and function of conventional T-cells. [UniProt]
Cellular Localization Cytoplasm, Nucleus. [UniProt]
Highlight Related products:
FOXP3 antibodies; FOXP3 Duos / Panels; Anti-Rabbit IgG secondary antibodies;
Related news:
Tumor-Infiltrating Lymphocytes (TILs)
Research Area Cell Biology and Cellular Response antibody; Gene Regulation antibody; Immune System antibody; Regulatory T cells Study antibody
Calculated MW 47 kDa
PTM Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation. [UniProt]

Images (11) Click the Picture to Zoom In

  • ARG53050 anti-FOXP3 antibody [SP97] WB image

    Western Blot: Tonsil stained with FOXP3 antibody [SP97] (ARG53050)

  • ARG53050 anti-FOXP3 antibody [SP97] IHC-P image

    Immunohistochemistry: Human B Cell Lymphoma stained with FOXP3 antibody [SP97] (ARG53050)

  • ARG53050 anti-FOXP3 antibody [SP97] IHC-P image

    Immunohistochemistry: Human Bladder Transitional Cell Carcinoma stained with FOXP3 antibody [SP97] (ARG53050)

  • ARG53050 anti-FOXP3 antibody [SP97] IHC-P image

    Immunohistochemistry: Human Bladder stained with FOXP3 antibody [SP97] (ARG53050)

  • ARG53050 anti-FOXP3 antibody [SP97] IHC-P image

    Immunohistochemistry: Human Cervical Squamous Cell Carcinoma stained with FOXP3 antibody [SP97] (ARG53050)

  • ARG53050 anti-FOXP3 antibody [SP97] IHC-P image

    Immunohistochemistry: Human Colon Adenocarcinoma stained with FOXP3 antibody [SP97] (ARG53050)

  • ARG53050 anti-FOXP3 antibody [SP97] IHC-P image

    Immunohistochemistry: Human Colon stained with FOXP3 antibody [SP97] (ARG53050)

  • ARG53050 anti-FOXP3 antibody [SP97] IHC-P image

    Immunohistochemistry: Human Lymph Node stained with FOXP3 antibody [SP97] (ARG53050)

  • ARG53050 anti-FOXP3 antibody [SP97] IHC-P image

    Immunohistochemistry: Human Pancreatic Adenocarcinoma stained with FOXP3 antibody [SP97] (ARG53050)

  • ARG53050 anti-FOXP3 antibody [SP97] IHC-P image

    Immunohistochemistry: Human Stomach Adenocarcinoma stained with FOXP3 antibody [SP97] (ARG53050)

  • ARG53050 anti-FOXP3 antibody [SP97] IHC-P image

    Immunohistochemistry: Human Thymus stained with FOXP3 antibody [SP97] (ARG53050)