ARG53050
anti-FOXP3 antibody [SP97]
anti-FOXP3 antibody [SP97] for IHC-Formalin-fixed paraffin-embedded sections and Human
Cell Biology and Cellular Response antibody; Gene Regulation antibody; Immune System antibody; Regulatory T cells Study antibody
Overview
| Product Description | Rabbit Monoclonal antibody [SP97] recognizes FOXP3 |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | IHC-P |
| Host | Rabbit |
| Clonality | Monoclonal |
| Clone | SP97 |
| Isotype | IgG |
| Target Name | FOXP3 |
| Antigen Species | Human |
| Immunogen | Synthetic peptide corresponding to C-terminus of human FOXP3 protein. |
| Conjugation | Un-conjugated |
| Alternate Names | FOXP3; Forkhead Box P3; DIETER; XPID; AIID; PIDX; IPEX; JM2; Immune Dysregulation, Polyendocrinopathy, Enteropathy, X-Linked; Forkhead Box Protein P3; SCURFIN; Immunodeficiency, Polyendocrinopathy, Enteropathy, X-Linked; FOXP3delta7; Scurfin |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|
| Application Note | IHC-P: Antigen Retrieval: Boil tissue section in EDTA buffer, pH 8.0 for 10 min followed by cooling at room temperature for 20 min. Incubation Time: 20 min at RT. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| Positive Control | Tonsil |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified by protein A/G |
| Buffer | PBS (pH 7.6), 1% BSA and < 0.1% Sodium azide |
| Preservative | < 0.1% Sodium azide |
| Stabilizer | 1% BSA |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | FOXP3 |
| Gene Full Name | forkhead box P3 |
| Background | The protein encoded by this gene is a member of the forkhead/winged-helix family of transcriptional regulators. Defects in this gene are the cause of immunodeficiency polyendocrinopathy, enteropathy, X-linked syndrome (IPEX), also known as X-linked autoimmunity-immunodeficiency syndrome. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008] |
| Function | Transcriptional regulator which is crucial for the development and inhibitory function of regulatory T-cells (Treg). [UniProt] Plays an essential role in maintaining homeostasis of the immune system by allowing the acquisition of full suppressive function and stability of the Treg lineage, and by directly modulating the expansion and function of conventional T-cells. [UniProt] |
| Cellular Localization | Cytoplasm, Nucleus. [UniProt] |
| Highlight | Related products: FOXP3 antibodies; FOXP3 Duos / Panels; Anti-Rabbit IgG secondary antibodies; Related news: Tumor-Infiltrating Lymphocytes (TILs) |
| Research Area | Cell Biology and Cellular Response antibody; Gene Regulation antibody; Immune System antibody; Regulatory T cells Study antibody |
| Calculated MW | 47 kDa |
| PTM | Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation. [UniProt] |
Images (11) Click the Picture to Zoom In
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ARG53050 anti-FOXP3 antibody [SP97] WB image
Western Blot: Tonsil stained with FOXP3 antibody [SP97] (ARG53050)
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ARG53050 anti-FOXP3 antibody [SP97] IHC-P image
Immunohistochemistry: Human B Cell Lymphoma stained with FOXP3 antibody [SP97] (ARG53050)
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ARG53050 anti-FOXP3 antibody [SP97] IHC-P image
Immunohistochemistry: Human Bladder Transitional Cell Carcinoma stained with FOXP3 antibody [SP97] (ARG53050)
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ARG53050 anti-FOXP3 antibody [SP97] IHC-P image
Immunohistochemistry: Human Bladder stained with FOXP3 antibody [SP97] (ARG53050)
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ARG53050 anti-FOXP3 antibody [SP97] IHC-P image
Immunohistochemistry: Human Cervical Squamous Cell Carcinoma stained with FOXP3 antibody [SP97] (ARG53050)
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ARG53050 anti-FOXP3 antibody [SP97] IHC-P image
Immunohistochemistry: Human Colon Adenocarcinoma stained with FOXP3 antibody [SP97] (ARG53050)
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ARG53050 anti-FOXP3 antibody [SP97] IHC-P image
Immunohistochemistry: Human Colon stained with FOXP3 antibody [SP97] (ARG53050)
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ARG53050 anti-FOXP3 antibody [SP97] IHC-P image
Immunohistochemistry: Human Lymph Node stained with FOXP3 antibody [SP97] (ARG53050)
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ARG53050 anti-FOXP3 antibody [SP97] IHC-P image
Immunohistochemistry: Human Pancreatic Adenocarcinoma stained with FOXP3 antibody [SP97] (ARG53050)
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ARG53050 anti-FOXP3 antibody [SP97] IHC-P image
Immunohistochemistry: Human Stomach Adenocarcinoma stained with FOXP3 antibody [SP97] (ARG53050)
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ARG53050 anti-FOXP3 antibody [SP97] IHC-P image
Immunohistochemistry: Human Thymus stained with FOXP3 antibody [SP97] (ARG53050)
