ARG22000
anti-CD11b antibody [M1/70]
anti-CD11b antibody [M1/70] for Blocking,Flow cytometry,ICC/IF,IHC-Frozen sections,Immunoprecipitation and Human,Mouse,Baboon,Rhesus Monkey
MDSC Marker antibody; Myeloid-derived suppressor cell antibody
Publication3
Overview
| Product Description | Rat Monoclonal antibody [M1/70] recognizes CD11b |
|---|---|
| Tested Reactivity | Hu, Ms, Bb, R. Mk |
| Tested Application | BL, FACS, ICC/IF, IHC-Fr, IP |
| Specificity | Mouse/Human/Rhesus/Baboon CD11b |
| Host | Rat |
| Clonality | Monoclonal |
| Clone | M1/70 |
| Isotype | IgG2b, kappa |
| Target Name | CD11b |
| Antigen Species | Mouse |
| Immunogen | C57BL/10 Mouse splenic T cells and concanavalin A-activated C57BL/10 splenocytes |
| Conjugation | Un-conjugated |
| Alternate Names | MAC1A; CR3A; CR-3 alpha chain; Cell surface glycoprotein MAC-1 subunit alpha; Integrin alpha-M; MAC-1; CD11 antigen-like family member B; Leukocyte adhesion receptor MO1; MO1A; SLEB6; Neutrophil adherence receptor; CD antigen CD11b; CD11B |
Application Instructions
| Application Suggestion |
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| Application Note | IHC-P: PLP fixation is recommended for optimal results. Antigen Retrieval: Digested with 0.0025% Trypsin in 0.l% Calcium chloride (pH 7.8), for 5 minutes. (PMID: 7868861) * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Buffer | BBS (pH 8.2) |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | ITGAM |
| Gene Full Name | integrin, alpha M (complement component 3 receptor 3 subunit) |
| Background | CD11b (integrin alpha M chain): Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This I-domain containing alpha integrin combines with the beta 2 chain (ITGB2) to form a leukocyte-specific integrin referred to as macrophage receptor 1 ('Mac-1'), or inactivated-C3b (iC3b) receptor 3 ('CR3'). The alpha M beta 2 integrin is important in the adherence of neutrophils and monocytes to stimulated endothelium, and also in the phagocytosis of complement coated particles. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2009] |
| Function | CD11b: Integrin ITGAM/ITGB2 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles and pathogens (PubMed:9558116, PubMed:20008295). It is identical with CR-3, the receptor for the iC3b fragment of the third complement component. It probably recognizes the R-G-D peptide in C3b. Integrin ITGAM/ITGB2 is also a receptor for fibrinogen, factor X and ICAM1. It recognizes P1 and P2 peptides of fibrinogen gamma chain. Regulates neutrophil migration (PubMed:28807980). In association with beta subunit ITGB2/CD18, required for CD177-PRTN3-mediated activation of TNF primed neutrophils (PubMed:21193407). May regulate phagocytosis-induced apoptosis in extravasated neutrophils. May play a role in mast cell development. Required with TYROBP/DAP12 in microglia to control production of microglial superoxide ions which promote the neuronal apoptosis that occurs during brain development. [UniProt] |
| Highlight | Related Antibody Duos and Panels: ARG30335 Mouse MDSC Marker Antibody Duo ARG30336 Human MDSC Marker Antibody Duo Related products: CD11b antibodies; CD11b Duos / Panels; Anti-Rat IgG secondary antibodies; Related news: New antibody panels and duos for Tumor immune microenvironment Anti-SerpinB9 therapy, a new strategy for cancer therapy |
| Research Area | MDSC Marker antibody; Myeloid-derived suppressor cell antibody |
| Calculated MW | 127 kDa |
Images (1) Click the Picture to Zoom In
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ARG22000 anti-CD11b antibody [M1/70] IHC-Fr image
Immunohistochemistry: Cryosection of Mouse lymph node stained with ARG22000 anti-CD11b antibody [M1/70] (green, A) and anti-Mouse CD8 antibody (red, B). Merged image in C.
Specific References
Mac-1 deficiency ameliorates pressure overloaded heart failure through inhibiting macrophage polarization and activation
IHC-Fr, WB / Mouse
Integrin CD11b Contributes to Hypertension and Vascular Dysfunction Through Mediating Macrophage Adhesion and Migration
WB / Mouse
CD11b mediates hypertensive cardiac remodeling by regulating macrophage infiltration and polarization
Mouse / IHC-P, WB
