ARG81351

HMGB1 ELISA Kit

Datasheet

Publication18

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96 wells

625.00

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Overview

Product Description	ARG81351 HMGB1 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human/Mouse/Rat HMGB1 in serum samples.
Tested Reactivity	Hu, Ms, Rat
Tested Application	ELISA
Specificity	No significant cross-reactivity or interference with recombinant human HMGB2 was observed.
Target Name	HMGB1
Conjugation	HRP
Conjugation Note	Substrate: TMB and read at 450 nm.
Sensitivity	0.4 ng/ml
Sample Type	Serum
Standard Range	0.625 - 20 ng/ml
Sample Volume	50 µl
Alternate Names	HMG-1; High mobility group protein B1; High mobility group protein 1; HMG1; SBP-1; HMG3

Application Instructions

Assay Time	~ 2 hours

Properties

Form	96 well
Storage Instruction	Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note	For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links	GeneID: 15289 Mouse HMGB1 GeneID: 3146 Human HMGB1 Swiss-port # P09429 Human High mobility group protein B1 Swiss-port # P63158 Mouse High mobility group protein B1
Gene Symbol	HMGB1
Gene Full Name	high mobility group box 1
Background	HMGB1 is a protein that belongs to the High Mobility Group-box superfamily. The encoded non-histone, nuclear DNA-binding protein regulates transcription, and is involved in organization of DNA. This protein plays a role in several cellular processes, including inflammation, cell differentiation and tumor cell migration. Multiple pseudogenes of this gene have been identified. Alternative splicing results in multiple transcript variants that encode the same protein. [provided by RefSeq, Sep 2015]
Function	HMGB1 is a DNA binding protein. It associates with chromatin and has the ability to bend DNA. Binds preferentially single-stranded DNA. Involved in V(D)J recombination by acting as a cofactor of the RAG complex. Acts by stimulating cleavage and RAG protein binding at the 23 bp spacer of conserved recombination signal sequences (RSS). [UniProt]
Highlight	Related products: HMGB1 antibodies; HMGB1 ELISA Kits; HMGB1 Duos / Panels; HMGB1 recombinant proteins; Related news: HMGB1, a biomarker and therapeutic target in COVID-19 Total solution for HMGB1 research HMGB1 in inflammation Inflammatory Cytokines HMGB1 ELISA Kit for your research Detecting the DAMPs in cancer therapy by HMGB1 ELISA kit New HMGB1 neutralizing antibody is released Detecting exosomal HMGB1 for ICD research Related poster download: HMGB1 Pathway.pdf New ELISA data calculation tool: Simplify the ELISA analysis by GainData
PTM	Phosphorylated at serine residues. Phosphorylation in both NLS regions is required for cytoplasmic translocation followed by secretion (PubMed:17114460). Acetylated on multiple sites upon stimulation with LPS (PubMed:22801494). Acetylation on lysine residues in the nuclear localization signals (NLS 1 and NLS 2) leads to cytoplasmic localization and subsequent secretion (By similarity). Acetylation on Lys-3 results in preferential binding to DNA ends and impairs DNA bending activity (By similarity). Reduction/oxidation of cysteine residues Cys-23, Cys-45 and Cys-106 and a possible intramolecular disulfide bond involving Cys-23 and Cys-45 give rise to different redox forms with specific functional activities in various cellular compartments: 1- fully reduced HMGB1 (HMGB1C23hC45hC106h), 2- disulfide HMGB1 (HMGB1C23-C45C106h) and 3- sulfonyl HMGB1 (HMGB1C23soC45soC106so). Poly-ADP-ribosylated by PARP1 when secreted following stimulation with LPS (By similarity). In vitro cleavage by CASP1 is liberating a HMG box 1-containing peptide which may mediate immunogenic activity; the peptide antagonizes apoptosis-induced immune tolerance (PubMed:24474694). Can be proteolytically cleaved by a thrombin:thrombomodulin complex; reduces binding to heparin and proinflammatory activities (By similarity). [UniProt]

Images (1) Click the Picture to Zoom In

ARG81351 HMGB1 ELISA Kit standard curve image

ARG81351 HMGB1 ELISA Kit results of a typical standard run with optical density reading at 450 nm.

Specific References

Myeloid TLR4 signaling promotes post-injury withdrawal resolution of murine liver fibrosis

ELISA / Mouse / Serum

Yoichi Takimoto et al.
iScience., (2023)

Ferritin-Based Nanocomposite Hydrogel Promotes Tumor Penetration and Enhances Cancer Chemoimmunotherapy

ELISA / Mouse / Cell culture supernatant

Rong Liu et al.
Adv Sci (Weinh), (2023)

Nanoparticle-Mediated CD47-SIRPα Blockade and Calreticulin Exposure for Improved Cancer Chemo-Immunotherapy

ELISA / Mouse / Cell culture supernatant

Jia-Qi Luo et al.
ACS Nano., (2023)

Tissue adhesive indocyanine green-locking granular gel-mediated photothermal therapy combined with checkpoint inhibitor for preventing postsurgical recurrence and metastasis of colorectal cancer

ELISA / Mouse/Cell culture supernatant

Yuan Zeting et al.
Bioengineering & Translational Medicine, (2023)

(-)-Guaiol triggers immunogenic cell death and inhibits tumor growth in non-small cell lung cancer

ELISA / Human / Cell culture supernatant

Xiaohui Yang et al.
Mol Cell Biochem, (2022)

MicroRNA-200b-3p as a biomarker for diagnosis and survival prognosis of multiple organ dysfunction syndrome caused by acute paraquat poisoning

ELISA / Human / Serum

Minggang Ding et al.
Hum Exp Toxicol., (2022)

Enhancement of antitumor immunotherapy using mitochondria-targeted cancer cell membrane-biomimetic MOF-mediated sonodynamic therapy and checkpoint blockade immunotherapy

ELISA / Mouse/ Cell culture supernatant

Jiali Luo et al.
Journal of Nanobiotechnology, (2022)

Pegylated Liposomal Mitoxantrone Modulates Tumor Immune Landscape to Boost Pd-L1 Blockade Therapy

ELISA / Mouse / Tumor interstitial fluid

Mengwen Huang et al.
SSRN., (2022)

Elevation of circulating neutrophil extracellular traps, interleukin (IL)-8, IL-22, and vascular endothelial growth factor in patients with venomous snake mamushi (Gloydius blomhoffii) bites

ELISA / Human / Serum

Yasuaki Ogura et al.
J Dermatol., (2022)

Magnoflorine Alleviates “M1” Polarized Macrophage-Induced Intervertebral Disc Degeneration Through Repressing the HMGB1/Myd88/NF-κB Pathway and NLRP3 Inflammasome

ELISA / Human / Cell culture supernatant

Feng Zhao et al.
Front Pharmacol., (2021)

Association Between Serum Alarmin Levels and Disease-specific Indices in Patients With Anti-neutrophil Cytoplasmic Antibody-associated Vasculitis.

ELISA / Human / Serum

Sung Soo Ahn et al.
In Vivo., (2021)

Downregulation of high mobility group box 1 enhances the radiosensitivity of non-small cell lung cancer by acting as a crucial target of microRNA-107

ELISA / Human serum

Lu Bai et al.
Exp Ther Med., (2021)

HMGB1, anti-HMGB1 antibodies, and ratio of HMGB1/anti-HMGB1 antibodies as diagnosis indicator in fever of unknown origin.

ELISA / Human / Serum

Mingkun Chen et al.
Sci Rep., (2021)

Efficacy of Recombinant Human-Soluble Thrombomodulin for Severe Acute Pancreatitis in a Rat Experimental Model.

ELISA / Rat / Serum

Matsumoto Masataka et al.
Pancreas., (2020)

Pannexin1 Channel Inhibitor (10panx) Protects Against Transient Focal Cerebral Ischemic Injury by Inhibiting RIP3 Expression and Inflammatory Response in Rats.

ELISA / Rat

Wei Ruili et al.
Neuroscience., (2020)

HMGB1-associated necroptosis and Kupffer cells M1 polarization underlies remote liver injury induced by intestinal ischemia/reperfusion in rats.

ELISA / Rat / Serum

Wen Shihong et al.
FASEB J., (2020)

Involvement of Receptor for Advanced Glycation Endproducts in Hypertensive Disorders of Pregnancy

ELISA / Human

Akasaka Juria et al.
Int J Mol Sci., (2019)

呼吸道过敏性疾病患儿血清 EDN 与总 IgE 水平的相关性分析

ELISA / Human

殷皓 et al.
中国免疫学杂志, (2019)