ARG65560

anti-CD33 antibody [WM53]

anti-CD33 antibody [WM53] for CyTOF®-candidate,Flow cytometry,Functional study,ICC/IF,IHC-Frozen sections,Immunoprecipitation,Western blot and Human,Primates

Developmental Biology antibody; Immune System antibody; Human MDSC Marker antibody; Myeloid-derived suppressor cell antibody

Overview

Product Description Mouse Monoclonal antibody [WM53] recognizes CD33
Tested Reactivity Hu, NHuPrm
Tested Application CyTOF®-candidate, FACS, FuncSt, ICC/IF, IHC-Fr, IP, WB
Specificity The clone WM53 reacts with CD33, a 67 kDa type I transmembrane glycoprotein (immunoglobulin superfamily) expressed on myeloid progenitors, monocytes, granulocytes, dendritic cells and mast cells; it is absent on platelets, lymphocytes, erythrocytes and hematopoietic stem cells.
HLDA IV; WS Code M-505
Host Mouse
Clonality Monoclonal
Clone WM53
Isotype IgG1
Target Name CD33
Antigen Species Human
Immunogen Human AML cells
Conjugation Un-conjugated
Alternate Names p67; Sialic acid-binding Ig-like lectin 3; SIGLEC-3; CD antigen CD33; gp67; Siglec-3; Myeloid cell surface antigen CD33; SIGLEC3

Application Instructions

Application Suggestion
Tested Application Dilution
CyTOF®-candidateAssay-dependent
FACS1 - 4 µg/ml
FuncStAssay-dependent
ICC/IFAssay-dependent
IHC-Fr2 µg/ml
IPAssay-dependent
WBAssay-dependent
Application Note IHC-Fr: Acetone fixation.
Functional application: Induction of cytokine production.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purified from cell culture supernatant by protein-A affinity chromatography.
Purity > 95% (by SDS-PAGE)
Buffer PBS (pH 7.4) and 15 mM Sodium azide
Preservative 15 mM Sodium azide
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 945 Human CD33

Swiss-port # P20138 Human Myeloid cell surface antigen CD33

Gene Symbol CD33
Gene Full Name CD33 molecule
Background CD33 is a transmembrane protein of the sialic acid-binding immunoglobulin-like lectin (Siglec) family. It belongs to the immunoreceptor tyrosine-based inhibitory motif (ITIM)-containing molecules able of recruiting protein tyrosine phosphatases SHP-1 and SHP-2 to signal assemblies; these ITIMs are also used for ubiquitin-mediated removal of the receptor from the cell surface. CD33 is expressed on cells of myelomonocytic lineage, binds sialic acid residues in N- and O-glycans on cell surfaces, and is a therapeutic target for acute myeloid leukemia.
Function CD33: Sialic-acid-binding immunoglobulin-like lectin (Siglec) that plays a role in mediating cell-cell interactions and in maintaining immune cells in a resting state (PubMed:10611343, PubMed:15597323, PubMed:11320212). Preferentially recognizes and binds alpha-2,3- and more avidly alpha-2,6-linked sialic acid-bearing glycans (PubMed:7718872). Upon engagement of ligands such as C1q or syalylated glycoproteins, two immunoreceptor tyrosine-based inhibitory motifs (ITIMs) located in CD33 cytoplasmic tail are phosphorylated by Src-like kinases such as LCK (PubMed:28325905, PubMed:10887109). These phosphorylations provide docking sites for the recruitment and activation of protein-tyrosine phosphatases PTPN6/SHP-1 and PTPN11/SHP-2 (PubMed:10556798, PubMed:10206955, PubMed:10887109). In turn, these phosphatases regulate downstream pathways through dephosphorylation of signaling molecules (PubMed:10206955, PubMed:10887109). One of the repressive effect of CD33 on monocyte activation requires phosphoinositide 3-kinase/PI3K (PubMed:15597323). [UniProt]
Highlight Related Antibody Duos and Panels:
ARG30336 Human MDSC Marker Antibody Duo
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CD33 antibodies; CD33 ELISA Kits; CD33 Duos / Panels; Anti-Mouse IgG secondary antibodies;
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Research Area Developmental Biology antibody; Immune System antibody; Human MDSC Marker antibody; Myeloid-derived suppressor cell antibody
Calculated MW 40 kDa
PTM Phosphorylation of Tyr-340 is involved in binding to PTPN6 and PTPN11. Phosphorylation of Tyr-358 is involved in binding to PTPN6.

Images (4) Click the Picture to Zoom In

  • ARG65560 anti-CD33 antibody [WM53] IHC-Fr image

    Immunohistochemistry: Frozen section of Human colon tissue stained with ARG65560 anti-CD33 antibody [WM53].

  • ARG65560 anti-CD33 antibody [WM53] CyTOF image

    CyTOF: Human peripheral blood stained with ARG65560 anti-CD33 antibody [WM53] (149Sm). Singlet cells were gated for data analysis.

  • ARG65560 anti-CD33 antibody [WM53] FACS image

    Flow Cytometry: Separation of Human CD33 positive Monocytes (red) from Human CD33 negative Lymphocytes (black-dashed). Human peripheral blood stained with ARG65560 anti-CD33 antibody [WM53], followed by incubation with APC labelled Goat anti-Mouse secondary antibody.

  • ARG65560 anti-CD33 antibody [WM53] FACS image

    Flow Cytometry: PBMC stained with ARG65560 anti-CD33 antibody [WM53] at 0.5 µg/10^6 cells (right histogram) or isotype control (left histogram), followed by incubation with PE labelled secondary antibody.

Clone References

Highly multiparametric analysis by mass cytometry.

FACS / Human

Ornatsky O et al.
J Immunol Methods.,  (2010)

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A study of CD33 (SIGLEC-3) antigen expression and function on activated human T and NK cells: two isoforms of CD33 are generated by alternative splicing.

Hernández-Caselles T et al.
J Leukoc Biol.,  (2006)

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Mobilization of bone marrow-derived stem cells after myocardial infarction and left ventricular function.

Leone AM et al.
Eur Heart J.,  (2005)

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Macrophages expressing triggering receptor expressed on myeloid cells-1 are underrepresented in the human intestine.

Schenk M et al.
J Immunol.,  (2005)

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Expression of the myeloid-associated marker CD33 is not an exclusive factor for leukemic plasmacytoid dendritic cells.

Garnache-Ottou F et al.
Blood.,  (2005)

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