ARG55553
anti-EMR1 F4 / 80 antibody [BM8]
anti-EMR1 F4 / 80 antibody [BM8] for Flow cytometry,IHC-Frozen sections,Western blot and Human,Mouse
Mouse Inflammatory Cell Marker antibody; Macrophage Marker antibody
Overview
Product Description | Rat Monoclonal antibody [BM8] recognizes EMR1 F4 / 80 |
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Tested Reactivity | Hu, Ms |
Tested Application | FACS, IHC-Fr, WB |
Specificity | Recognizes a 125 kDa of mouse extracellular macrophage membrane molecule, highly restricted to mature macrophage subpopulations residing in tissue. Species Crossreactivity: human heart macrophages. |
Host | Rat |
Clonality | Monoclonal |
Clone | BM8 |
Isotype | IgG2a |
Target Name | EMR1 F4 / 80 |
Antigen Species | Mouse |
Immunogen | BALB/c macrophages obtained from 14 day old bone marrow cell cultures. |
Conjugation | Un-conjugated |
Alternate Names | EGF-like module-containing mucin-like hormone receptor-like 1; EMR1 hormone receptor; Emr1; DD7A5-7; Adhesion G protein-coupled receptor E1; Ly71; EGF-like module receptor 1; Gpf480; F4/80; Cell surface glycoprotein F4/80; EGF-TM7; TM7LN3 |
Application Instructions
Application Suggestion |
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Application Note | WB: 1:50 detects F4/80 in mouse bone marrow derived macrophages under non-reduced conditions. Reduction with 2-mercaptoethanol destroys BM8 antigen. Flow Cytometry: Fix with 1% paraformaldehyde. IHC-Fr: Tissue embedded in OCT Tissue Tec, fixed with acetone for 10 minutes at RT. Incubate with PBS, 0.02M sodium azide, 0.1% H2O2 for 10 minutes at RT to destroy endogenous peroxidase. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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Positive Control | IHC-Fr: Spleen |
Properties
Form | Liquid |
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Purification | Purification with Protein G. |
Buffer | PBS, 0.02% Sodium azide and 0.1% BSA |
Preservative | 0.02% Sodium azide |
Stabilizer | 0.1% BSA |
Concentration | 0.1 mg/ml |
Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
Database Links |
Swiss-port # Q14246 Human Adhesion G protein-coupled receptor E1 Swiss-port # Q61549 Mouse Adhesion G protein-coupled receptor E1 |
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Gene Symbol | Adgre1 |
Gene Full Name | adhesion G protein-coupled receptor E1 |
Background | EMR1 F4/80 has a domain resembling seven transmembrane G protein-coupled hormone receptors (7TM receptors) at its C-terminus. The N-terminus of the encoded protein has six EGF-like modules, separated from the transmembrane segments by a serine/threonine-rich domain, a feature reminiscent of mucin-like, single-span, integral membrane glycoproteins with adhesive properties. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2012] |
Function | EMR1 F4/80: Orphan receptor involved in cell adhesion and probably in cell-cell interactions specifically involving cells of the immune system. May play a role in regulatory T-cells (Treg) development. [UniProt] |
Highlight | Related products: EMR1 F4/80 antibodies; EMR1 F4/80 Duos / Panels; Anti-Rat IgG secondary antibodies; Related news: Exploring Antiviral Immune Response |
Research Area | Mouse Inflammatory Cell Marker antibody; Macrophage Marker antibody |
Calculated MW | 98 kDa |