ARG81185
Human HMGB1 ELISA Kit
Human HMGB1 ELISA Kit for ELISA and Human
Publication30
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG81185-001 | antibody-coated microplate | 12 X 8 strips | 4°C |
| ARG81185-002 | Standard (100 ng) | 1 vial (lyophilized) | 4°C, store at -20 °C after reconstitution |
| ARG81185-003 | Standard reconstitution buffer | 1.1 ml | 4°C |
| ARG81185-004 | HRP-antibody Conjugate (200X) | 115 μl | 4°C |
| ARG81185-005 | Sample & Antibody diluent buffer | 50 ml | 4°C |
| ARG81185-006 | 10X Wash buffer | 20 ml | 4°C |
| ARG81185-007 | TMB substrate | 12 ml (ready-to-use) | 4°C (Protect from light) |
| ARG81185-008 | STOP solution | 6 ml | 4°C |
Overview
| Product Description | ARG81185 Human HMGB1 ELISA Kit is an Enzyme Immunoassay kit in simple steps with good precision for the quantification of Human HMGB1 in plasma and cell culture supernatants. |
|---|---|
| Tested Reactivity | Hu |
| Predict Reactivity | Ms, Rat |
| Tested Application | ELISA |
| Specificity | This Human HMGB1 ELISA kit detect natural and recombinant Human HMGB1 protein and the cross-reactivity with HMGB2 is lower than <2%. |
| Target Name | HMGB1 |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 0.3 ng/ml |
| Sample Type | Plasma and cell culture supernatants. |
| Standard Range | 0.3125 - 20 ng/ml |
| Sample Volume | 100 µl |
| Precision | less than 5.2% |
| Alternate Names | HMG-1; High mobility group protein B1; High mobility group protein 1; HMG1; SBP-1; HMG3 |
Application Instructions
| Assay Time | Overnight |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | HMGB1 |
| Gene Full Name | high mobility group box 1 |
| Background | HMGB1 is a protein that belongs to the High Mobility Group-box superfamily. The encoded non-histone, nuclear DNA-binding protein regulates transcription, and is involved in organization of DNA. This protein plays a role in several cellular processes, including inflammation, cell differentiation and tumor cell migration. Multiple pseudogenes of this gene have been identified. Alternative splicing results in multiple transcript variants that encode the same protein. [provided by RefSeq, Sep 2015] |
| Function | HMGB1 is a DNA binding protein. It associates with chromatin and has the ability to bend DNA. Binds preferentially single-stranded DNA. Involved in V(D)J recombination by acting as a cofactor of the RAG complex. Acts by stimulating cleavage and RAG protein binding at the 23 bp spacer of conserved recombination signal sequences (RSS). [UniProt] |
| Highlight | Related products: HMGB1 antibodies; HMGB1 ELISA Kits; HMGB1 Duos / Panels; HMGB1 recombinant proteins; Related news: HMGB1, a biomarker and therapeutic target in COVID-19 Total solution for HMGB1 research HMGB1 in inflammation Inflammatory Cytokines HMGB1 ELISA Kit for your research Detecting the DAMPs in cancer therapy by HMGB1 ELISA kit New HMGB1 neutralizing antibody is released Detecting exosomal HMGB1 for ICD research Related poster download: HMGB1 Pathway.pdf New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| PTM | Phosphorylated at serine residues. Phosphorylation in both NLS regions is required for cytoplasmic translocation followed by secretion (PubMed:17114460). Acetylated on multiple sites upon stimulation with LPS (PubMed:22801494). Acetylation on lysine residues in the nuclear localization signals (NLS 1 and NLS 2) leads to cytoplasmic localization and subsequent secretion (By similarity). Acetylation on Lys-3 results in preferential binding to DNA ends and impairs DNA bending activity (By similarity). Reduction/oxidation of cysteine residues Cys-23, Cys-45 and Cys-106 and a possible intramolecular disulfide bond involving Cys-23 and Cys-45 give rise to different redox forms with specific functional activities in various cellular compartments: 1- fully reduced HMGB1 (HMGB1C23hC45hC106h), 2- disulfide HMGB1 (HMGB1C23-C45C106h) and 3- sulfonyl HMGB1 (HMGB1C23soC45soC106so). Poly-ADP-ribosylated by PARP1 when secreted following stimulation with LPS (By similarity). In vitro cleavage by CASP1 is liberating a HMG box 1-containing peptide which may mediate immunogenic activity; the peptide antagonizes apoptosis-induced immune tolerance (PubMed:24474694). Can be proteolytically cleaved by a thrombin:thrombomodulin complex. (By similarity). |
Images (2) Click the Picture to Zoom In
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ARG81185 Human HMGB1 ELISA Kit standard curve image
ARG81185 Human HMGB1 ELISA Kit results of a typical standard run with optical density reading at 450 nm.
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ARG81185 Human HMGB1 ELISA Kit specificity testing image
Recombinant HMGB1 and recombinant HMGB2 were used for ARG81185 Human HMGB1 ELISA Kit specificity test. The data showed no significant cross-reactivity or interference with recombinant human HMGB2.
Specific References
Modulation of immune-responses by DSF/Cu enhances the anti-tumor effects of DTX for metastasis breast cancer
ELISA / Cell culture supernatants / Human
High mobility group box 1 promotes endometriosis under hypoxia by regulating inflammation and autophagy in vitro and in vivo
ELISA / Human / Cell culture supernatant
Association Between Clinicopathological Parameters and S100A8/A9 Expression According to Smoking History in Patients With Non-small Cell Lung Cancer
ELISA / Human / serum
Brachyury-targeted immunotherapy combined with gemcitabine against head and neck cancer
ELISA / Human / Cell culture supernatant
Turning anecdotal irradiation-induced anti-cancer immune responses into reproducible in situ cancer vaccines via disulfiram/copper-mediated enhanced immunogenic cell death of breast cancer cells
ELISA / Human / Cell culture supernatant
HMGB1 induces macrophage pyroptosis and serves as a promising diagnostic marker for chronic endometritis
ELISA / Human / Cell culture supernatant
NLRP3 inflammasome activation and symptom burden in KRAS-mutated CMML patients is reverted by IL-1 blocking therapy
ELISA / Human / Plasma
Identifying TNF and IL6 as potential hub genes and targeted drugs associated with scleritis: A bio-informative report
ELISA / Human / serum
HMGB1 induces macrophage pyroptosis in chronic endometritis
ELISA / Human / Cell culture supernatant
Initial Myeloid Cell Status Is Associated with Clinical Outcomes of Renal Cell Carcinoma
ELISA / Human / Plasma
AIM/CD5L ameliorates autoimmune arthritis by promoting removal of inflammatory DAMPs at the lesions
ELISA / Human / Serum
Tumor-targeted redox-regulating and antiangiogenic phototherapeutics nanoassemblies for self-boosting phototherapy
ELISA / Human / Cell culture supernatant
Diclofenac-Induced Cytotoxicity in Direct and Indirect Co-Culture of HepG2 Cells with Differentiated THP-1 Cells
ELISA / Human / Cell culture supernatant
Tumor-targeting oxidative stress nanoamplifiers as anticancer nanomedicine with immunostimulating activity
ELISA / Human / Cell culture supernatant
Zinc-Loaded Black Phosphorus Multifunctional Nanodelivery System Combined With Photothermal Therapy Have the Potential to Treat Prostate Cancer Patients Infected With COVID-19
ELISA / Human / Cell culture supernatant
Doxorubicin-loaded Black Phosphorus Multifunctional Nanodelivery System Combined With Photothermal Therapy Promotes Immunogenic Death of Prostate Cancer PC-3 cells
ELISA / Human / Cell culture supernatant
Autophagy-based unconventional secretion of HMGB1 in glioblastoma promotes chemosensitivity to temozolomide through macrophage M1-like polarization
ELISA / Human / Cell culture supernatant
Self-deliverable and self-immolative prodrug nanoassemblies as tumor targeted nanomedicine with triple cooperative anticancer actions
ELISA / Human / Plasma
Soluble P2X7 Receptor Is Elevated in the Plasma of COVID-19 Patients and Correlates With Disease Severity
ELISA / Human / Cell culture supernatant
ADAM10 inhibits the interaction between IL-17 and HMGB1 in Buerger's disease.
ELISA / Human / Plasma
The effect of IGFBP-4 treatment on immunogenic cell death in A549 cells and its mechanism.
ELISA / Human / Cell culture supernatant
Tumor-Targeting H2O2-Responsive Photosensitizing Nanoparticles with Antiangiogenic and Immunogenic Activities for Maximizing Anticancer Efficacy of Phototherapy
ELISA / Human
The role of HMGB1 on TDI-induced NLPR3 inflammasome activation via ROS/NF-κB pathway in HBE cells
ELISA / Human / Cell culture supernatant
Increased Serum Concentrations of High Mobility Group Box 1 (HMGB1) Protein in Children with Autism Spectrum Disorder.
ELISA / Human / Serum
Tumor-derived HMGB1 induces CD62L dim neutrophil polarization and promotes lung metastasis in triple-negative breast cancer.
ELISA / Human / Serum
Indirect regulation of HMGB1 release by gasdermin D.
ELISA / Mouse / Plasma
High-mobility Group Protein B1: A Predictive Biomarker for Hepatic Encephalopathy After Transjugular Intrahepatic Portosystemic Shunt.
ELISA / Human / Serum
High Mobility Group box-1 Protects Against Aflatoxin G 1-induced Pulmonary Epithelial Cell Damage in the Lung Inflammatory Environment.
ELISA / Human / Cell culture supernatant
Evidence of necroptosis in osteoarthritic disease: investigation of blunt mechanical impact as possible trigger in regulated necrosis.
ELISA / Human / Cell culture supernatant
High-mobility group box 1 protein modulated Proliferation and Radioresistance in Esophageal Squamous Cell Carcinoma.
ELISA / Human
| Title | Download Link |
|---|---|
| ARG81185 Human HMGB1 ELISA Kit User's manual |
 Download
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