ARG83005
arigoPLEX® Human CTL/NK activation Multiplex ELISA Kit (IFN gamma, TNF alpha, Perforin, Granzyme B)
arigoPLEX® Human CTL/NK activation Multiplex ELISA Kit (IFN gamma, TNF alpha, Perforin, Granzyme B) for ELISA and Human
Publication1
Component
| Cat No | Component Name | Package | |
|---|---|---|---|
| ARG83005-01 | Antibody Coated Microplate | 8x12 strips | 4°C |
| ARG83005-02 | Standards Mixture | 2 vials | 4°C |
| ARG83005-03 | 100X Antibody Conjugate Mixture | 120 μl | ≤ -20°C |
| ARG83005-04 | 1000X HRP-Streptavidin Solution | 15 μl | 4°C |
| ARG83005-05 | Standard / Sample Diluent Buffer | 30 ml | 4°C |
| ARG83005-06 | Antibody Diluent Buffer | 35 ml | 4°C |
| ARG83005-07 | 10X Wash Buffer | 50 ml | 4°C |
| ARG83005-08 | TMB Substrate | 12 ml | 4°C (protect from light) |
| ARG83005-09 | STOP solution | 12 ml | 4°C |
| ARG83005-10 | Plate sealer | 3 adhesive strips |
Overview
| Product Description | ARG83005 arigoPLEX® Human CTL/NK activation Multiplex ELISA Kit (IFN gamma, TNF alpha, Perforin, Granzyme B) is an Enzyme Immunoassay kit for the quantification of Human Cytotoxic T cell Cytokine (IFN gamma, TNF alpha, Perforin, Granzyme B) in serum, plasma and cell culture supernatants. See all Multiplex ELISA kits |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Target Name | CTL/NK activation |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | IFN gamma: 15.625 pg/ml TNF alpha: 23.44 pg/ml Perforin: 93.75 pg/ml Granzyme B: 15.625 pg/ml |
| Sample Type | Serum, plasma and cell culture supernatants. |
| Standard Range | IFN gamma: 31.25 - 1000 pg/ml TNF alpha: 46.875 - 1500 pg/ml Perforin: 187.5 - 6000 pg/ml Granzyme B: 31.25 - 1000 pg/ml |
| Sample Volume | 50 µl |
Application Instructions
| Assay Time | 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store components at 4°C or -20°C. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Gene Symbol | IFNG; TNF; PRF1; GZMB |
|---|---|
| Highlight | Related Product: IFN-gamma antibodies; TNF-alpha antibodies; Perforin antibodies; Granzyme B antibodies; Related news: CTL/NK Activation Multiplex ELISA Kit is launched; Perforin and Granzyme B; Validating cytotoxicity of γδT cells in NSCLC; |
Images (5) Click the Picture to Zoom In
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Antibodies Coating Pattern In Microtiter Plate of ARG83005 arigoPLEX® Human CTL/NK Activation Multiplex ELISA Kit (IFN gamma, TNF alpha, Perforin, Granzyme B)
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ARG83005 arigoPLEX® Human CTL/NK Activation Multiplex ELISA Kit (IFN gamma, TNF alpha, Perforin, Granzyme B) standard curve image
ARG83005 arigoPLEX® Human CTL/NK Activation Multiplex ELISA Kit (IFN gamma, TNF alpha, Perforin, Granzyme B) results of a typical standard for Human IFN gamma run with optical density reading at 450 nm.
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ARG83005 arigoPLEX® Human CTL/NK Activation Multiplex ELISA Kit (IFN gamma, TNF alpha, Perforin, Granzyme B) standard curve image
ARG83005 arigoPLEX® Human CTL/NK Activation Multiplex ELISA Kit (IFN gamma, TNF alpha, Perforin, Granzyme B) results of a typical standard for Human TNF alpha run with optical density reading at 450 nm.
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ARG83005 arigoPLEX® Human CTL/NK activation Multiplex ELISA Kit (IFN gamma, TNF alpha, Perforin, Granzyme B) standard curve imageA
ARG83005 arigoPLEX® Human CTL/NK Activation Multiplex ELISA Kit (IFN gamma, TNF alpha, Perforin, Granzyme B) results of a typical standard for Human Perforin run with optical density reading at 450 nm.
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ARG83005 arigoPLEX® Human CTL/NK Activation Multiplex ELISA Kit (IFN gamma, TNF alpha, Perforin, Granzyme B) standard curve image
ARG83005 arigoPLEX® Human CTL/NK Activation Multiplex ELISA Kit (IFN gamma, TNF alpha, Perforin, Granzyme B) results of a typical standard for Human Granzyme B run with optical density reading at 450 nm.
Specific References
The cytotoxicity of γδT cells in non-small cell lung cancer mediated via coordination of the BCL-2 and AKT pathways
ELISA / Human / Cell culture supernatants
| Title | Download Link |
|---|---|
| ARG83005 arigoPLEX® Human CTL/NK activation Multiplex ELISA Kit (IFN gamma, TNF alpha, Perforin, Granzyme B) User manual |
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