ARG67000

anti-CD3 antibody

Datasheet

Ordering Information

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Price (USD)

100 μl

389.00

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Datasheet

Overview

Product Description	Rabbit Polyclonal antibody recognizes CD3
Tested Reactivity	Hu, Ms
Predict Reactivity	Rat
Tested Application	FACS, ICC/IF, IHC-P, WB
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Target Name	CD3
Antigen Species	Human
Immunogen	Synthetic peptide corresponding to the intracellular domain of Human CD3 epsilon.
Conjugation	Un-conjugated
Alternate Names	T-cell surface antigen T3/Leu-4 epsilon chain; T3E; TCRE; T-cell surface glycoprotein CD3 epsilon chain; IMD18; CD antigen CD3e

Application Instructions

Application Suggestion

Tested Application	Dilution
FACS	Assay-dependent
ICC/IF	1:100 - 1:400
IHC-P	1:50 - 1:300
WB	1:500 - 1:1000

Application Note

IHC-P: Antigen Retrieval: Boil tissue section in Citrate buffer (pH 6.0).
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Positive Control

Jurkat; Thymus tissue; Spleen; Tonsil carcinoma

Observed Size

23 kDa

Properties

Form	Liquid
Purification	Affinity purified.
Buffer	100 mM Tris Glycine (pH 7.0), 0.025% ProClin 300 and 20% Glycerol.
Preservative	0.025% ProClin 300
Stabilizer	20% Glycerol
Storage Instruction	For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note	For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links	GeneID: 12501 Mouse CD3E GeneID: 916 Human CD3E Swiss-port # P07766 Human T-cell surface glycoprotein CD3 epsilon chain Swiss-port # P22646 Mouse T-cell surface glycoprotein CD3 epsilon chain
Gene Symbol	CD3E
Gene Full Name	CD3e molecule, epsilon (CD3-TCR complex)
Background	CD3 subunit complex is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins superfamily encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation.
Function	CD3: Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways (PubMed:2470098). In addition of this role of signal transduction in T-cell activation, CD3E plays an essential role in correct T-cell development. Initiates the TCR-CD3 complex assembly by forming the two heterodimers CD3D/CD3E and CD3G/CD3E. Participates also in internalization and cell surface down-regulation of TCR-CD3 complexes via endocytosis sequences present in CD3E cytosolic region (PubMed:10384095, PubMed:26507128). [UniProt]
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Research Area	Cancer antibody; Developmental Biology antibody; Immune System antibody; Lymphocyte Marker antibody; Inflammatory Cell Marker antibody; T-cell Marker antibody; T-cell infiltration Study antibody; Tumor-infiltrating Lymphocyte Study antibody
Calculated MW	23 kDa

Images (5) Click the Picture to Zoom In

ARG67000 anti-CD3 antibody ICC/IF image

Immunofluorescence: Jurkat cells were fixed with 4% paraformaldehyde at RT for 10 min, permeabilized with 0.1% NP-40 at RT for 10 min and then cells were blocked with 5% BSA at RT for 30 min and stained with ARG67000 anti-CD3 antibody (red) at 1:200 dilution at 4°C for overnight. DAPI (blue) was used for nuclear staining.
ARG67000 anti-CD3 antibody IHC-P(Human) image

Immunohistochemistry: Paraffin-embedded Human Tonsil carcinoma tissue stained with ARG67000 anti-CD3 antibody at 1:200 dilution. Antigen Retrieval: Boil tissue section in Citrate buffer (pH 6.0).
ARG67000 anti-CD3 antibody WB image

Western blot: 40 µg of Jurkat cell and Mouse Thymus tissue lysate stained with ARG67000 anti-CD3 antibody at 1:1000 dilution, overnight at 4°C.
ARG67000 anti-CD3 antibody FACS image

Flow Cytometry: Human whole blood stained with ARG67000 anti-CD3 antibody at 1:100 dilution (grey area).
ARG67000 anti-CD3 antibody IHC-P(Mouse) image

Immunohistochemistry: Paraffin-embedded Mouse spleen tissue stained with ARG67000 anti-CD3 antibody at 1:200 dilution. Antigen Retrieval: Boil tissue section in Citrate buffer (pH 6.0).