ARG30346

Myofibroblast / Fibrosis Antibody Panel

Datasheet

Publication35

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Price (USD)

1 kit

399.00

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Datasheet
Publication (35)

Component

Cat No	Component Name	Host clonality	Reactivity	Application	Package
ARG66381	anti-alpha smooth muscle Actin antibody [SQab18108]	Rabbit mAb	Hu, Ms, Rat, AGMK, Bov, Ctl, Dog, Pig, Zfsh	FACS, ICC/IF, IHC-Fr, IHC-P, WB	20 μl
ARG21965	anti-Collagen I antibody, pre-adsorbed	Goat pAb	Bov, Cat, Chk, Dog, Elp, Gpig, Hm, Hu, Ms, Pig, Rb, Rat, Sheep	EM, ELISA, FLISA, FACS, ICC/IF, IHC-P, IHC-Fr, IP, WB	20 μg
ARG66162	anti-Fibronectin antibody	Mouse mAb	Hu, Ms, Rat	IHC-P, WB	20 μg

Overview

Product Description	Myofibroblasts are a differentiated cell type with a phenotype between a fibroblast and a smooth muscle cell. They play a physiological role in wound healing and fibrosis. Myofibroblast/Fibrosis Antibody Panel is an all-in-one solution to make the research of myofibroblast and fibrosis easy and economical. This antibody panel comprises the myofibroblast markers including smooth muscle actin, collagen I, and fibronectin antibodies. It is the best solution to study myofibroblast and fibrosis in samples from human, mouse or rat. Related news: New antibody panels for Myofibroblasts and CAFs
Target Name	Myofibroblast / Fibrosis

Product Description

Myofibroblasts are a differentiated cell type with a phenotype between a fibroblast and a smooth muscle cell. They play a physiological role in wound healing and fibrosis.

Myofibroblast/Fibrosis Antibody Panel is an all-in-one solution to make the research of myofibroblast and fibrosis easy and economical. This antibody panel comprises the myofibroblast markers including smooth muscle actin, collagen I, and fibronectin antibodies. It is the best solution to study myofibroblast and fibrosis in samples from human, mouse or rat.

Related news:
New antibody panels for Myofibroblasts and CAFs

Target Name

Myofibroblast / Fibrosis

Properties

Storage Instruction	For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note	For laboratory research only, not for drug, diagnostic or other use.

Images (24) Click the Picture to Zoom In

ARG21965 anti-Collagen I antibody, pre-adsorbed IHC-P image

Immunohistochemistry: Rabbit osteochondral stained with ARG21965 anti-Collagen I antibody, pre-adsorbed at 1:100 dilution.

From Wenli Dai et al. Enhanced osteochondral repair with hyaline cartilage formation using an extracellular matrix-inspired natural scaffold (2023), doi: 10.1016/j.scib.2023.07.050, Fig. 7a.
ARG66162 anti-Fibronectin antibody WB image

Western blot: 20 µg of Huh7 and Huh7 shHMGB1 cell lysates stained with ARG66162 anti-Fibronectin antibody (1:1000), ARG55914 anti-E-cadherin antibody (1:1000) and ARG65636 anti-HMGB1 antibody (1:2000).
ARG66381 anti-alpha smooth muscle Actin antibody [SQab18108] IHC-Fr image

Immunohistochemistry: Mouse liver and Mouse aorta stained with ARG66381 anti-alpha smooth muscle Actin antibody [SQab18108] at 1:100 dilution.

From Masahiro Terasawa et al. Cells (2023), doi: 10.3390/cells12222666, Fig. 2A.
ARG66381 anti-alpha smooth muscle Actin antibody [SQab18108] WB image

Western blot: 30 μg of NRK-49F cells treated with TGF beta 1 (10 ng/ml) for 0~48 hours. Cell lysates were stained with ARG66381 anti-alpha smooth muscle Actin antibody [SQab18108] at 1:2000 dilution, overnight at 4°C.
ARG21965 anti-Collagen I antibody, pre-adsorbed ICC/IF image

Immunofluorescence: Rabbit synovium-derived mesenchymal stem cell stained with ARG21965 anti-Collagen I antibody, pre-adsorbed at 1:1000 dilution.

From Zong Li e et al. Chemical Engineering Journal, (2023), doi: 10.1016/j.cej.2023.145209, Fig. 4B.
ARG21965 anti-Collagen I antibody, pre-adsorbed IHC-P image

Immunohistochemistry: Canine aorta mesothelial stained with ARG21965 anti-Collagen I antibody, pre-adsorbed at 1:500 dilution.

From Masakazu Shimada et al. PLoS One. (2022), doi: 35061786, Fig. 1.
ARG66381 anti-alpha smooth muscle Actin antibody [SQab18108] ICC/IF image

Immunofluorescence: NRK-49F cells treated with TGF beta 1 (10 ng/ml) for 0~48 hours. Cells were fixed with 4% PFA for 15 min at room temperature and permeabilizated by 0.5% Triton X-100. Cells were stained with ARG66381 anti-alpha smooth muscle Actin antibody [SQab18108] at 1:200 dilution, overnight at 4°C.
ARG21965 anti-Collagen I antibody, pre-adsorbed ICC/IF image

Immunofluorescence: Human fibroblasts were stained with ARG21965 anti-Collagen I antibody (pre-adsorbed).
ARG66381 anti-alpha smooth muscle Actin antibody [SQab18108] FACS image

Flow Cytometry: HeLa cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% TritonX-100 for 15 min. The cells were then stained with ARG66381 anti-alpha smooth muscle Actin antibody [SQab18108] (red) at 1:100 dilution in 1x PBS/1% BSA for 30 min at 4°C, followed by Alexa Fluor® 488 labelled secondary antibody. Unlabelled sample (green) was used as a control.
ARG21965 anti-Collagen I antibody, pre-adsorbed IHC-Fr image

Immunohistochemistry: Frozen MC4R-KO Mouse liver section was stained with ARG21965 anti-Collagen I antibody (pre-adsorbed), anti-F4/80 antibody followed by secondary antibodies and DAPI.
ARG66162 anti-Fibronectin antibody IHC-P image

Immunohistochemistry: Paraffin-embedded Rat liver tissue stained with ARG66162 anti-Fibronectin antibody at 1:200 (4°C, overnight). Antigen Retrieval: Boil tissue section in Sodium citrate buffer (pH 6.0) for 20 min. Secondary antibody was diluted at 1:200 (RT, 30min). Negative control: Secondary antibody only.
ARG66381 anti-alpha smooth muscle Actin antibody [SQab18108] IHC-P image

Immunohistochemistry: Formalin-fixed and paraffin-embedded placenta stained with ARG66381 anti-alpha smooth muscle Actin antibody [SQab18108] at 1:2000 dilution. Antigen Retrieval: Heat mediated was performed using Tris/EDTA buffer (pH 9.0).
ARG21965 anti-Collagen I antibody, pre-adsorbed WB image

Western blot: Purified Human Type I Collagen stained with ARG21965 anti-Collagen I antibody (pre-adsorbed).
ARG66381 anti-alpha smooth muscle Actin antibody [SQab18108] WB image

Western blot: 10 µg of HeLa, HaCat, 293, LnCap, PC-3 and T47D cell lysates stained with ARG66381 anti-alpha smooth muscle Actin antibody [SQab18108] at 1:2000 dilution.
ARG21965 anti-Collagen I antibody, pre-adsorbed IHC-P image

Immunohistochemistry: Paraffin embedded Rat kidney section post uninephrectomy was stained with ARG21965 anti-Collagen I antibody (pre-adsorbed) followed by a secondary antibody and AEC.
ARG66162 anti-Fibronectin antibody IHC-P image

Immunohistochemistry: Paraffin-embedded Mouse liver tissue stained with ARG66162 anti-Fibronectin antibody at 1:200 (4°C, overnight). Antigen Retrieval: Boil tissue section in Sodium citrate buffer (pH 6.0) for 20 min. Secondary antibody was diluted at 1:200 (RT, 30min). Negative control: Secondary antibody only.
ARG66162 anti-Fibronectin antibody IHC image

Immunohistochemistry: Human appendix tissue stained with ARG66162 anti-Fibronectin antibody (red) at 1:200 (4°C, overnight). Picture A: Target. Picture B: DAPI. Picture C: merge of A and B.
ARG66162 anti-Fibronectin antibody IHC image

Immunohistochemistry: Human appendix tissue stained with ARG66162 anti-Fibronectin antibody (red) at 1:200 (4°C, overnight). Picture A: Target. Picture B: DAPI. Picture C: merge of A and B.
ARG66162 anti-Fibronectin antibody IHC image

Immunohistochemistry: Human appendix tissue stained with ARG66162 anti-Fibronectin antibody (red) at 1:200 (4°C, overnight). Picture A: Target. Picture B: DAPI. Picture C: merge of A and B.
ARG66162 anti-Fibronectin antibody IHC image

Immunohistochemistry: Mouse spleen tissue stained with ARG66162 anti-Fibronectin antibody (red) at 1:200 (4°C, overnight). Picture A: Target. Picture B: DAPI. Picture C: merge of A and B.
ARG66162 anti-Fibronectin antibody IHC image

Immunohistochemistry: Mouse spleen tissue stained with ARG66162 anti-Fibronectin antibody (red) at 1:200 (4°C, overnight). Picture A: Target. Picture B: DAPI. Picture C: merge of A and B.
ARG66162 anti-Fibronectin antibody IHC image

Immunohistochemistry: Mouse spleen tissue stained with ARG66162 anti-Fibronectin antibody (red) at 1:200 (4°C, overnight). Picture A: Target. Picture B: DAPI. Picture C: merge of A and B.
ARG66381 anti-alpha smooth muscle Actin antibody [SQab18108] WB image

Western blot: 10 µg of MDCK, MDBK, Pig heart, Zebrafish and Cos-7 lysates stained with ARG66381 anti-alpha smooth muscle Actin antibody [SQab18108] at 1:2000 dilution.
ARG66381 anti-alpha smooth muscle Actin antibody [SQab18108] WB image

Western blot: 10 µg of Mouse brain, Rat kidney and Rat liver lysates stained with ARG66381 anti-alpha smooth muscle Actin antibody [SQab18108] at 1:2000 dilution.

Specific References

Formononetin ameliorates isoproterenol induced cardiac fibrosis through improving mitochondrial dysfunction

ARG66381: WB / Mouse

Lei Qian et al.
Biomed Pharmacother., (2024)

Resveratrol attenuates inflammation and fibrosis in rheumatoid arthritis-associated interstitial lung disease via the AKT/TMEM175 pathway

ARG21965: WB, IHC-P / Human, Mouse

Nannan Liu et al.
J Transl Med., (2024)

Melatonin alleviates aging-related heart failure through melatonin receptor 1A/B knockout in mice

ARG66381: IHC-P / Mouse

Zhenyu Feng et al.
Heliyon, (2024)

Functional meniscus reconstruction with biological and biomechanical heterogeneities through topological self-induction of stem cells

ARG21965: ICC/IF / Rabbit

Mingze Du et al.
Bioact Mater, (2024)

The potential of brimonidine for myopia treatment: Targeting MMP-2 to regulate choroidal thickness and control eye growth

ARG21965: IHC-Fr, WB / Guinea pig

Aiqun Xiang et al.
Heliyon, (2024)

Bone marrow stromal and anterior cruciate ligament remnant cell co-culture-derived extracellular vesicles promote cell activity in both cell types

ARG21965: ICC/IF / Rabbit

Sung-Yen Lin et al.
J Cell Mol Med., (2024)

PCSK9 inhibitors ameliorate arterial stiffness in ACS patients: evidences from mendelian randomization, cohort studies and basic experiments

ARG66381: WB / Mouse

Linghao Xu et al.
Preprint, (2024)

The mechano-chemical circuit in fibroblasts and dendritic cells drives basal cell proliferation in psoriasis

ARG21965: WB / Mouse

Jingwei Jiang et al.
Cell Rep., (2024)

Ultrasound-Controlled Delivery of Growth Factor-Loaded Cerasomes Combined with Polycaprolactone Scaffolds Seeded with Bone Marrow Mesenchymal Stem Cells for Biomimetic Tendon-to-Bone Interface Engineering

ARG21965: IHC-P / Rabbit

Cancan Du et al.
ACS Appl Mater Interfaces., (2024)

Oxamate alleviates silicotic fibrosis in mice by inhibiting senescence of alveolar type II epithelial cells

ARG21965: WB / Mouse

Liu Wenjing et al.
Journal of Environmental and Occupational Medicine, (2024)

Mac-1 deficiency ameliorates pressure overloaded heart failure through inhibiting macrophage polarization and activation

ARG66381: IHC-P / Mouse

Qiu-Yue Lin et al.
BBA - Molecular Basis of Disease, (2024)

Effect of macrophage-to-myofibroblast transition on silicosis

ARG21965: ICC/IF, IHC-P,WB / Rat

Fei Geng et al.
Anim Models Exp Med., (2024)

Expression of elastin, FBXW2, fibrillin-1, and α-smooth muscle actin in damaged blood vessels of bovine legs

ARG66381: IHC-P / Bovine

Mari Akiyama et al.
Preprint, (2024)

Effect of lyophilized exosomes derived from umbilical cord stem cells on chronic anterior cruciate ligament cell injury

ARG21965: ICC/IF / Human

Hon-Lok Lo et al.
Preprint, (2024)

Mesenchymal Stem Cells Preconditioned with Hypoxia and Dexamethasone Promote Osteoblast Differentiation Under Stress Conditions

ARG21965: ICC/IF / Human

Miyako Shimasaki et al.
Journal of Medical Sciences, (2024)

Elastic Fibers and F-Box and WD-40 Domain-Containing Protein 2 in Bovine Periosteum and Blood Vessels

Cattle / ARG66381: IHC-P

Mari Akiyama et al.
Biomimetics (Basel)., (2023)

CD11b mediates hypertensive cardiac remodeling by regulating macrophage infiltration and polarization

Mouse / ARG66381: IHC-P

Yun-Long Zhang et al.
J Adv Res., (2023)

Vitamin E stabilizes iron and mitochondrial metabolism in pulmonary fibrosis

ARG66381: IHC-P / Mouse

Jing Chang et al.
Front Pharmacol., (2023)

Anterior cruciate ligament remnant preservation attenuates apoptosis and enhances the regeneration of hamstring tendon graft

ARG21965: ICC/IF / Rabbit

Cheng-Chang Lu et al.
Bone Joint Res., (2023)

Anti-Apoptosis Therapy for Meniscal Avascular Zone Repair: A Proof-of-Concept Study in a Lapine Model

ARG21965: IHC-P / Rabbit

Wenqiang Yan et al.
Bioengineering (Basel)., (2023)

Oral Administration of Rhamnan Sulfate from Monostroma nitidum Suppresses Atherosclerosis in ApoE-Deficient Mice Fed a High-Fat Diet

ARG66381: IFC-Fr / Mouse

Masahiro Terasawa et al.
Cells., (2023)

Enhanced osteochondral repair with hyaline cartilage formation using an extracellular matrix-inspired natural scaffold

ARG21965: IHC-P / Rabbit

Wenli Dai et al.
Sci Bull (Beijing)., (2023)

Regional specific tunable meniscus decellularized extracellular matrix (MdECM) reinforced bioink promotes anistropic meniscus regeneration

ARG21965: ICC/IF / Rabbit

Zong Li et al.
Chemical Engineering Journal, (2023)

"Two-birds-one-stone" colon-targeted nanomedicine treats ulcerative colitis via remodeling immune microenvironment and anti-fibrosis

ARG66381: WB / Mouse

Jiaxin Zhang et al.
J Nanobiotechnology, (2022)

Inhibition of angiogenesis and tumor progression of MK-0429, an integrin αvβ3 antagonist, on oral squamous cell carcinoma

ARG66381: IHC-P / Human

Takayuki Nakagawa et al.
J Cancer Res Clin Oncol ., (2022)

ZLN005 Alleviates In Vivo and In Vitro Renal Fibrosis via PGC-1α-Mediated Mitochondrial Homeostasis

ARG66381: ICC/IF / Human

Pengfei Zhu et al.
Pharmaceuticals (Basel)., (2022)

Anticancer Effects and Molecular Mechanisms of Apigenin in Cervical Cancer Cells

ARG66162: WB; IHC-P / Human

Ya-Hui Chen et al.
Cancers (Basel)., (2022)

Resveratrol Ameliorates Fibrosis in Rheumatoid Arthritis-Associated Interstitial Lung Disease via the Autophagy–Lysosome Pathway

ARG21965: WB / Mouse

Lanxin Bao et al.
Molecules, (2022)

Loss of MLKL ameliorates liver fibrosis by inhibiting hepatocyte necroptosis and hepatic stellate cell activation

ARG21965: IHC-P / Mouse

Ren Guo et al.
Theranostics, (2022)

Inhibition of Angiogenesis and Tumor Progression of Mk-0429, an Integrin Αvβ3 Antagonist, on Oral Squamous Cell Carcinoma

ARG66381: IHC-P / Human

Takayuki Nakagawa et al.
Research Square, (2022)

Blocking VCAM-1 ameliorates hypertensive cardiac remodeling by impeding macrophage infiltration

ARG66381: IHC-P / Mouse

Ze-Yang Qiu et al.
Front Pharmacol, (2022)

Effects of long-term and high-dose administration of glucocorticoids on the cranial cruciate ligament in healthy beagle dogs

ARG21965: IHC-P / Dog

Masakazu Shimada et al.
PLoS One., (2022)

A Transwell-Based Vascularized Model to Investigate the Effect of Interstitial Flow on Vasculogenesis

ARG21965: ICC/IF / Human

Pengwei Deng et al.
Bioengineering (Basel), (2022)

Vinpocetine Ameliorates Metabolic-Syndrome-Associated Bladder Overactivity in Fructose-Fed Rats by Restoring Succinate-Modulated cAMP Levels and Exerting Anti-Inflammatory Effects in the Bladder Detrusor Muscle

ARG21965: WB / Mouse

Wei-Chia Lee et al.
Biomedicines, (2022)

VEGF-C/VEGFR-3 axis protects against pressure-overload induced cardiac dysfunction through regulation of lymphangiogenesis.

ARG66381: IHC-P / Mouse

Qiu-Yue Lin et al.
Clin Transl Med., (2021)