ARG66381
anti-alpha smooth muscle Actin antibody [SQab18108]
anti-alpha smooth muscle Actin antibody [SQab18108] for Flow cytometry,ICC/IF,IHC-Frozen sections,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat,African green monkey,Bovine,Cattle,Dog,Pig,Zebrafish
Cell Biology and Cellular Response antibody; Controls and Markers antibody; Signaling Transduction antibody; Cancer-associated fibroblast antibody; Smooth Muscle Marker antibody; CAF Marker antibody; Mural cell Marker antibody; Myofibroblast Marker antibody; Angiogenesis Study antibody; Microvascular Density Study antibody
Publication16
Overview
| Product Description | Recombinant Rabbit Monoclonal antibody [SQab18108] recognizes alpha smooth muscle Actin |
|---|---|
| Tested Reactivity | Hu, Ms, Rat, AGMK, Bov, Ctl, Dog, Pig, Zfsh |
| Tested Application | FACS, ICC/IF, IHC-Fr, IHC-P, WB |
| Host | Rabbit |
| Clonality | Monoclonal |
| Clone | SQab18108 |
| Isotype | IgG |
| Target Name | alpha smooth muscle Actin |
| Antigen Species | Human |
| Immunogen | Synthetic peptide corresponding to residues on the N-terminus of Human alpha smooth muscle Actin. |
| Conjugation | Un-conjugated |
| Alternate Names | Cell growth-inhibiting gene 46 protein; MYMY5; ACTSA; Alpha-actin-2; Actin, aortic smooth muscle; AAT6 |
Application Instructions
| Application Suggestion |
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| Application Note | IHC-P: Antigen Retrieval: Heat mediated was performed using Tris/EDTA buffer (pH 9.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein A. |
| Buffer | PBS, 0.01% Sodium azide, 40% Glycerol and 0.05% BSA. |
| Preservative | 0.01% Sodium azide |
| Stabilizer | 40% Glycerol and 0.05% BSA |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | ACTA2 |
| Gene Full Name | actin, alpha 2, smooth muscle, aorta |
| Background | The protein encoded by this gene belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actins being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. This actin is an alpha actin that is found in skeletal muscle. Defects in this gene cause aortic aneurysm familial thoracic type 6. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Nov 2008] |
| Function | Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. [UniProt] |
| Highlight | Related Antibody Duos and Panels: ARG30322 Myofibroblast Differentiation Antibody Duo ARG30327 Microvascular Density Antibody Panel ARG30346 Myofibroblast / Fibrosis Antibody Panel ARG30347 CAF Marker Antibody Panel Related products: alpha smooth muscle Actin antibodies; alpha smooth muscle Actin Duos / Panels; Anti-Rabbit IgG secondary antibodies; Related news: New antibody panels for Myofibroblasts and CAFs New antibody panels and duos for Tumor immune microenvironment Anti-SerpinB9 therapy, a new strategy for cancer therapy |
| Research Area | Cell Biology and Cellular Response antibody; Controls and Markers antibody; Signaling Transduction antibody; Cancer-associated fibroblast antibody; Smooth Muscle Marker antibody; CAF Marker antibody; Mural cell Marker antibody; Myofibroblast Marker antibody; Angiogenesis Study antibody; Microvascular Density Study antibody |
| Calculated MW | 42 kDa |
| PTM | Oxidation of Met-46 and Met-49 by MICALs (MICAL1, MICAL2 or MICAL3) to form methionine sulfoxide promotes actin filament depolymerization. MICAL1 and MICAL2 produce the (R)-S-oxide form. The (R)-S-oxide form is reverted by MSRB1 and MSRB2, which promote actin repolymerization (By similarity). Monomethylation at Lys-86 (K84me1) regulates actin-myosin interaction and actomyosin-dependent processes. Demethylation by ALKBH4 is required for maintaining actomyosin dynamics supporting normal cleavage furrow ingression during cytokinesis and cell migration. (Microbial infection) Monomeric actin is cross-linked by V.cholerae toxins RtxA and VgrG1 in case of infection: bacterial toxins mediate the cross-link between Lys-52 of one monomer and Glu-272 of another actin monomer, resulting in formation of highly toxic actin oligomers that cause cell rounding (PubMed:19015515). The toxin can be highly efficient at very low concentrations by acting on formin homology family proteins: toxic actin oligomers bind with high affinity to formins and adversely affect both nucleation and elongation abilities of formins, causing their potent inhibition in both profilin-dependent and independent manners (PubMed:26228148). [UniProt] |
Images (8) Click the Picture to Zoom In
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ARG66381 anti-alpha smooth muscle Actin antibody [SQab18108] IHC-Fr image
Immunohistochemistry: Mouse liver and Mouse aorta stained with ARG66381 anti-alpha smooth muscle Actin antibody [SQab18108] at 1:100 dilution.
From Masahiro Terasawa et al. Cells (2023), doi: 10.3390/cells12222666, Fig. 2A.
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ARG66381 anti-alpha smooth muscle Actin antibody [SQab18108] WB image
Western blot: 30 μg of NRK-49F cells treated with TGF beta 1 (10 ng/ml) for 0~48 hours. Cell lysates were stained with ARG66381 anti-alpha smooth muscle Actin antibody [SQab18108] at 1:2000 dilution, overnight at 4°C.
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ARG66381 anti-alpha smooth muscle Actin antibody [SQab18108] ICC/IF image
Immunofluorescence: NRK-49F cells treated with TGF beta 1 (10 ng/ml) for 0~48 hours. Cells were fixed with 4% PFA for 15 min at room temperature and permeabilizated by 0.5% Triton X-100. Cells were stained with ARG66381 anti-alpha smooth muscle Actin antibody [SQab18108] at 1:200 dilution, overnight at 4°C.
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ARG66381 anti-alpha smooth muscle Actin antibody [SQab18108] FACS image
Flow Cytometry: HeLa cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% TritonX-100 for 15 min. The cells were then stained with ARG66381 anti-alpha smooth muscle Actin antibody [SQab18108] (red) at 1:100 dilution in 1x PBS/1% BSA for 30 min at 4°C, followed by Alexa Fluor® 488 labelled secondary antibody. Unlabelled sample (green) was used as a control.
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ARG66381 anti-alpha smooth muscle Actin antibody [SQab18108] IHC-P image
Immunohistochemistry: Formalin-fixed and paraffin-embedded placenta stained with ARG66381 anti-alpha smooth muscle Actin antibody [SQab18108] at 1:2000 dilution. Antigen Retrieval: Heat mediated was performed using Tris/EDTA buffer (pH 9.0).
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ARG66381 anti-alpha smooth muscle Actin antibody [SQab18108] WB image
Western blot: 10 µg of HeLa, HaCat, 293, LnCap, PC-3 and T47D cell lysates stained with ARG66381 anti-alpha smooth muscle Actin antibody [SQab18108] at 1:2000 dilution.
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ARG66381 anti-alpha smooth muscle Actin antibody [SQab18108] WB image
Western blot: 10 µg of MDCK, MDBK, Pig heart, Zebrafish and Cos-7 lysates stained with ARG66381 anti-alpha smooth muscle Actin antibody [SQab18108] at 1:2000 dilution.
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ARG66381 anti-alpha smooth muscle Actin antibody [SQab18108] WB image
Western blot: 10 µg of Mouse brain, Rat kidney and Rat liver lysates stained with ARG66381 anti-alpha smooth muscle Actin antibody [SQab18108] at 1:2000 dilution.
Customer's Feedback
Good
Review for anti-alpha smooth muscle Actin antibody [SQab18108]
Application：WB
Sample：Transfected B6 wild type (lung tissue)
Sample Loading Amount：30 μg
Primary Antibody Dilution Factor：1:1000
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
Excellent
Review for anti-alpha smooth muscle Actin antibody [SQab18108]
Application：IF/ICC
Sample：NRK-49F treated with TGF beta 1 (10 ng/ml) for 0~48 hours
Fixation Buffer：4% PFA
Fixation Time：15 min
Fixation Temperature： RT ºC
Permeabilization Buffer：0.5% Triton X-100
Primary Antibody Dilution Factor：1:200
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
Conjugation of Primary Antibody：Alexa Fluor 594
Excellent
Review for anti-alpha smooth muscle Actin antibody [SQab18108]
Application：WB
Sample：NRK-49F treated with TGF beta 1 (10 ng/ml) for 0~48 hours
Sample Loading Amount：30 μg
Primary Antibody Dilution Factor：1:2000
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
Specific References
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Formononetin ameliorates isoproterenol induced cardiac fibrosis through improving mitochondrial dysfunction
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ZLN005 Alleviates In Vivo and In Vitro Renal Fibrosis via PGC-1α-Mediated Mitochondrial Homeostasis
ICC/IF / Human
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Inhibition of angiogenesis and tumor progression of MK-0429, an integrin αvβ3 antagonist, on oral squamous cell carcinoma
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Blocking VCAM-1 ameliorates hypertensive cardiac remodeling by impeding macrophage infiltration
IHC-P / Mouse
VEGF-C/VEGFR-3 axis protects against pressure-overload induced cardiac dysfunction through regulation of lymphangiogenesis.
IHC-P / Mouse
Inhibition of miR-155-5p Exerts Anti-Fibrotic Effects in Silicotic Rats by Regulating Meprin α
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