ARG55205 anti-S100A4 antibody WB image

Western blot: 30 µg of A549 and Rat lung lysates stained with ARG55205 anti-S100A4 antibody at 1:500 dilution.

ARG66199 anti-Vimentin antibody [SQab1721] WB image

Western blot: 20 µg of HeLa and 293T cell lysates stained with ARG66199 anti-Vimentin antibody [SQab1721] at 1:1000 dilution.

ARG66381 anti-alpha smooth muscle Actin antibody [SQab18108] IHC-Fr image

Immunohistochemistry: Mouse liver and Mouse aorta stained with ARG66381 anti-alpha smooth muscle Actin antibody [SQab18108] at 1:100 dilution.

From Masahiro Terasawa et al. Cells (2023), doi: 10.3390/cells12222666, Fig. 2A.

ARG66381 anti-alpha smooth muscle Actin antibody [SQab18108] WB image

Western blot: 30 μg of NRK-49F cells treated with TGF beta 1 (10 ng/ml) for 0~48 hours. Cell lysates were stained with ARG66381 anti-alpha smooth muscle Actin antibody [SQab18108] at 1:2000 dilution, overnight at 4°C.

ARG55205 anti-S100A4 antibody ICC/IF image

Immunofluorescence: RAW264.7 cells stained with ARG55205 anti-S100A4 antibody at 1:100 dilution. Blue: DAPI for nuclear staining.

ARG66199 anti-Vimentin antibody [SQab1721] ICC/IF image

Immunofluorescence: HeLa cells were fixed with 4% paraformaldehyde for 30 min at RT, permeabilized with 0.1% Triton X-100 for 10 min at RT then blocked with 10% goat serum for 30 min at room temperature. Cells were stained with ARG66199 anti-Vimentin antibody [SQab1721] (green) at 1:25000 and 4°C. DAPI (blue) was used as the nuclear counter stain.

ARG66381 anti-alpha smooth muscle Actin antibody [SQab18108] ICC/IF image

Immunofluorescence: NRK-49F cells treated with TGF beta 1 (10 ng/ml) for 0~48 hours. Cells were fixed with 4% PFA for 15 min at room temperature and permeabilizated by 0.5% Triton X-100. Cells were stained with ARG66381 anti-alpha smooth muscle Actin antibody [SQab18108] at 1:200 dilution, overnight at 4°C.

ARG66701 anti-FAP / Fibroblast activation protein antibody ICC/IF image

Immunofluorescence: Formalin-fixed HeLa cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were stained with ARG66701 anti-FAP / Fibroblast activation protein antibody (red) in 3% BSA-PBS and incubated overnight at 4°C. DAPI (blue) for nuclear staining.

ARG66199 anti-Vimentin antibody [SQab1721] FACS image

Flow Cytometry: HeLa cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% TritonX-100 for 15 min. The cells were then stained with ARG66199 anti-Vimentin antibody [SQab1721] (red) at 1:500 dilution in 1x PBS/1% BSA for 30 min at 4°C, followed by Alexa Fluor® 488 labelled secondary antibody. Unlabelled sample (black) was used as a control.

ARG66381 anti-alpha smooth muscle Actin antibody [SQab18108] FACS image

Flow Cytometry: HeLa cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% TritonX-100 for 15 min. The cells were then stained with ARG66381 anti-alpha smooth muscle Actin antibody [SQab18108] (red) at 1:100 dilution in 1x PBS/1% BSA for 30 min at 4°C, followed by Alexa Fluor® 488 labelled secondary antibody. Unlabelled sample (green) was used as a control.

ARG55205 anti-S100A4 antibody IHC-P image

Immunohistochemistry: Paraffin-embedded Human breast stained with ARG55205 anti-S100A4 antibody at 1:100 dilution.

ARG66199 anti-Vimentin antibody [SQab1721] IHC-P image

Immunohistochemistry: Formalin‐fixed and paraffin‐embedded Human colon tissue stained with ARG66199 anti-Vimentin antibody [SQab1721] at 1:1000 dilution.

Antigen retrieval: Heat mediated was performed using Tris/EDTA buffer pH 9.0

ARG66381 anti-alpha smooth muscle Actin antibody [SQab18108] IHC-P image

Immunohistochemistry: Formalin-fixed and paraffin-embedded placenta stained with ARG66381 anti-alpha smooth muscle Actin antibody [SQab18108] at 1:2000 dilution. Antigen Retrieval: Heat mediated was performed using Tris/EDTA buffer (pH 9.0).

ARG66701 anti-FAP / Fibroblast activation protein antibody IHC-P image

Immunohistochemistry: Formalin-fixed and paraffin-embedded Human prostate cancer tissue. Antigen Retrieval: Heat mediation was performed in Sodium citrate buffer (pH 6.0). The tissue section was stained with ARG66701 anti-FAP / Fibroblast activation protein antibody at room temperature. The section was counterstained with haematoxylin and mounted with DPX.

ARG66381 anti-alpha smooth muscle Actin antibody [SQab18108] WB image

Western blot: 10 µg of HeLa, HaCat, 293, LnCap, PC-3 and T47D cell lysates stained with ARG66381 anti-alpha smooth muscle Actin antibody [SQab18108] at 1:2000 dilution.

ARG66701 anti-FAP / Fibroblast activation protein antibody WB image

Western blot: HeLa, MCF7, NIH/3T3, Mouse kidney and Rat liver lysates stained with ARG66701 anti-FAP / Fibroblast activation protein antibody.

ARG66199 anti-Vimentin antibody [SQab1721] IP image

Immunoprecipitation: 0.4 mg of HeLa cell lysate immunoprecipitated and stained with ARG66199 anti-Vimentin antibody [SQab1721]. 1) IP in HeLa whole cell lysate, 2) Rabbit IgG instead of primary antibody in HeLa whole cell lysate and 3) HeLa whole cell lysate, 10 μg (input).

ARG66199 anti-Vimentin antibody [SQab1721] WB image

Western blot: 20 µg of COLO205, HCT116, HT29 (Vimentin unexpression cell lines) and SW620 (Vimentin expression cell line). Cell lysates stained with ARG66199 anti-Vimentin antibody [SQab1721] at 1:1000 dilution.

ARG66381 anti-alpha smooth muscle Actin antibody [SQab18108] WB image

Western blot: 10 µg of MDCK, MDBK, Pig heart, Zebrafish and Cos-7 lysates stained with ARG66381 anti-alpha smooth muscle Actin antibody [SQab18108] at 1:2000 dilution.

ARG66381 anti-alpha smooth muscle Actin antibody [SQab18108] WB image

Western blot: 10 µg of Mouse brain, Rat kidney and Rat liver lysates stained with ARG66381 anti-alpha smooth muscle Actin antibody [SQab18108] at 1:2000 dilution.