ARG55205

anti-S100A4 antibody

anti-S100A4 antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat

Cancer antibody; Cell Biology and Cellular Response antibody; Signaling Transduction antibody
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Overview

Product Description Rabbit Polyclonal antibody recognizes S100A4
Tested Reactivity Hu, Ms, Rat
Tested Application ICC/IF, IHC-P, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name S100A4
Antigen Species Human
Immunogen Recombinant protein of Human S100A4 (Swiss: P26447)
Conjugation Un-conjugated
Alternate Names S100 calcium-binding protein A4; Placental calcium-binding protein; 42A; MTS1; FSP1; CAPL; Metastasin; Protein S100-A4; P9KA; 18A2; Protein Mts1; Calvasculin; PEL98

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1:50 - 1:200
IHC-PAssay-dependent
WB1:500 - 1:2000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol
Preservative 0.02% Sodium azide
Stabilizer 50% Glycerol
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 20198 Mouse S100A4

GeneID: 24615 Rat S100A4

GeneID: 6275 Human S100A4

Gene Symbol S100A4
Gene Full Name S100 calcium binding protein A4
Background The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in motility, invasion, and tubulin polymerization. Chromosomal rearrangements and altered expression of this gene have been implicated in tumor metastasis. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Jul 2008]
Highlight Related Antibody Duos and Panels:
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S100A4 antibodies; S100A4 Duos / Panels; Anti-Rabbit IgG secondary antibodies;
Related news:
New antibody panels for Myofibroblasts and CAFs
Research Area Cancer antibody; Cell Biology and Cellular Response antibody; Signaling Transduction antibody
Calculated MW 12 kDa

Images (3) Click the Picture to Zoom In

  • ARG55205 anti-S100A4 antibody WB image

    Western blot: 30 µg of A549 and Rat lung lysates stained with ARG55205 anti-S100A4 antibody at 1:500 dilution.

  • ARG55205 anti-S100A4 antibody ICC/IF image

    Immunofluorescence: RAW264.7 cells stained with ARG55205 anti-S100A4 antibody at 1:100 dilution. Blue: DAPI for nuclear staining.

  • ARG55205 anti-S100A4 antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Human breast stained with ARG55205 anti-S100A4 antibody at 1:100 dilution.

Customer's Feedback

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Review for anti-S100A4 antibody

Application:WB

Sample:A549 and Rat lung

Sample Loading Amount:30 µg

Primary Antibody Dilution Factor:1:500

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

Specific References

Vitamin D Attenuates Loss of Endothelial Biomarker Expression in Cardio-Endothelial Cells.

IHC-P / Rat

Lai Chi-Cheng et al.
Int J Mol Sci.,  (2020)

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国产多孔钽对MG63细胞中Ⅰ型胶原、谷氨酰转氨酶2和钙结合蛋白A4表达的影响

WB / Human

Chen Jingjing et al.
Chinese Journal of Tissue Engineering Research,  (2019)

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