ARG55689
anti-HDGF antibody
anti-HDGF antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Overview
Product Description | Rabbit Polyclonal antibody recognizes HDGF |
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Tested Reactivity | Hu |
Tested Application | ICC/IF, IHC-P, WB |
Host | Rabbit |
Clonality | Polyclonal |
Isotype | IgG |
Target Name | HDGF |
Antigen Species | Human |
Immunogen | KLH-conjugated synthetic peptide corresponding to aa. 171-197 (C-terminus) of Human HDGF. |
Conjugation | Un-conjugated |
Alternate Names | HMG1L2; HMG-1L2; HDGF; High mobility group protein 1-like 2; Hepatoma-derived growth factor |
Application Instructions
Application Suggestion |
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Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
Positive Control | HeLa |
Properties
Form | Liquid |
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Purification | This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
Buffer | PBS and 0.09% (W/V) Sodium azide. |
Preservative | 0.09% (W/V) Sodium azide. |
Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
Database Links | |
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Gene Symbol | HDGF |
Gene Full Name | hepatoma-derived growth factor |
Background | HDGF is a member of the hepatoma-derived growth factor family. The encoded protein has mitogenic and DNA-binding activity and may play a role in cellular proliferation and differentiation. High levels of expression of this gene enhance the growth of many tumors. This gene was thought initially to be located on chromosome X; however, that location has been determined to correspond to a related pseudogene. Alternatively spliced transcript variants encoding distinct isoforms have been described. [provided by RefSeq, Jan 2016] |
Function | HDGF Isoform 1: Acts as a transcriptional repressor (PubMed:17974029). Has mitogenic activity for fibroblasts (PubMed:11751870, PubMed:26845719). Heparin-binding protein (PubMed:15491618). Isoform 2: Does not have mitogenic activity for fibroblasts (PubMed:26845719). Does not bind heparin (PubMed:26845719). Isoform 3: Has mitogenic activity for fibroblasts (PubMed:26845719). Heparin-binding protein (PubMed:26845719). [UniProt] |
Cellular Localization | Cytoplasm. Nucleus. |
Highlight | Related products: HDGF antibodies; HDGF ELISA Kits; Anti-Rabbit IgG secondary antibodies; Related news: The most sensitive human HDGF ELISA kit The role of HDGF in tumor angiogenesis |
Calculated MW | 27 kDa |
PTM | Sumoylated with SUMO1. Sumoylation prevents binding to chromatin. |
Images (3) Click the Picture to Zoom In
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ARG55689 anti-HDGF antibody ICC/IF image
Immunofluorescence: SK-BR-3 cells stained with ARG55689 anti-HDGF antibody (green). DAPI (blue) for nuclear staining.
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ARG55689 anti-HDGF antibody IHC-P image
Immunohistochemistry: Formalin-fixed and paraffin-embedded Human hepatocarcinoma tissue stained with ARG55689 anti-HDGF antibody.
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ARG55689 anti-HDGF antibody WB image
Western blot: 35 µg of HeLa cell lysate stained with ARG55689 anti-HDGF antibody.