ARG54705
anti-ACAT1 antibody
anti-ACAT1 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse
Cancer antibody; Controls and Markers antibody; Metabolism antibody; Signaling Transduction antibody
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Publication1
Overview
Product Description | Rabbit Polyclonal antibody recognizes ACAT1 |
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Tested Reactivity | Hu, Ms |
Predict Reactivity | Mk |
Tested Application | IHC-P, WB |
Host | Rabbit |
Clonality | Polyclonal |
Isotype | IgG |
Target Name | ACAT1 |
Antigen Species | Human |
Immunogen | KLH-conjugated synthetic peptide corresponding to aa. 296-329 (C-terminus) of Human ACAT1 (NP_000010.1). |
Conjugation | Un-conjugated |
Alternate Names | EC 2.3.1.9; Acetoacetyl-CoA thiolase; MAT; T2; ACAT; THIL; Acetyl-CoA acetyltransferase, mitochondrial |
Application Instructions
Application Suggestion |
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Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
Purification | This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
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Buffer | PBS and 0.09% (W/V) Sodium azide |
Preservative | 0.09% (W/V) Sodium azide |
Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
Database Links |
Swiss-port # P24752 Human Acetyl-CoA acetyltransferase, mitochondrial Swiss-port # Q8QZT1 Mouse Acetyl-CoA acetyltransferase, mitochondrial |
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Gene Symbol | ACAT1 |
Gene Full Name | acetyl-CoA acetyltransferase 1 |
Background | This gene encodes a mitochondrially localized enzyme that catalyzes the reversible formation of acetoacetyl-CoA from two molecules of acetyl-CoA. Defects in this gene are associated with 3-ketothiolase deficiency, an inborn error of isoleucine catabolism characterized by urinary excretion of 2-methyl-3-hydroxybutyric acid, 2-methylacetoacetic acid, tiglylglycine, and butanone. [provided by RefSeq, Feb 2009] |
Function | Plays a major role in ketone body metabolism. [From Uniprot] |
Cellular Localization | Mitochondrion. |
Highlight | Related products: ACAT1 antibodies; Anti-Rabbit IgG secondary antibodies; Related news: Cholesterol, the weakness of anaplastic large cell lymphoma (ALCL) |
Research Area | Cancer antibody; Controls and Markers antibody; Metabolism antibody; Signaling Transduction antibody |
Calculated MW | 45 kDa |
PTM | Succinylation at Lys-268, adjacent to a coenzyme A binding site. Desuccinylated by SIRT5 (By similarity). |
Images (2) Click the Picture to Zoom In
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ARG54705 anti-ACAT1 antibody WB image
Western blot: 30 µg of 1) HepG2, 2) SW480, 3) 293T, 4) MDAMB231, and 5) MEF lysate stained with ARG54705 anti-ACAT1 antibody at 1:500 dilution.
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ARG54705 anti-ACAT1 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human hepatocarcinoma tissue stained with ARG54705 anti-ACAT1 antibody.
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