ARG54705
anti-ACAT1 antibody
anti-ACAT1 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse
Cancer antibody; Controls and Markers antibody; Metabolism antibody; Signaling Transduction antibody
Publication1
Overview
| Product Description | Rabbit Polyclonal antibody recognizes ACAT1 |
|---|---|
| Tested Reactivity | Hu, Ms |
| Predict Reactivity | Mk |
| Tested Application | IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Target Name | ACAT1 |
| Antigen Species | Human |
| Immunogen | KLH-conjugated synthetic peptide corresponding to aa. 296-329 (C-terminus) of Human ACAT1 (NP_000010.1). |
| Conjugation | Un-conjugated |
| Alternate Names | EC 2.3.1.9; Acetoacetyl-CoA thiolase; MAT; T2; ACAT; THIL; Acetyl-CoA acetyltransferase, mitochondrial |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Purification | This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
|---|---|
| Buffer | PBS and 0.09% (W/V) Sodium azide |
| Preservative | 0.09% (W/V) Sodium azide |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P24752 Human Acetyl-CoA acetyltransferase, mitochondrial Swiss-port # Q8QZT1 Mouse Acetyl-CoA acetyltransferase, mitochondrial |
|---|---|
| Gene Symbol | ACAT1 |
| Gene Full Name | acetyl-CoA acetyltransferase 1 |
| Background | This gene encodes a mitochondrially localized enzyme that catalyzes the reversible formation of acetoacetyl-CoA from two molecules of acetyl-CoA. Defects in this gene are associated with 3-ketothiolase deficiency, an inborn error of isoleucine catabolism characterized by urinary excretion of 2-methyl-3-hydroxybutyric acid, 2-methylacetoacetic acid, tiglylglycine, and butanone. [provided by RefSeq, Feb 2009] |
| Function | Plays a major role in ketone body metabolism. [From Uniprot] |
| Cellular Localization | Mitochondrion. |
| Highlight | Related products: ACAT1 antibodies; Anti-Rabbit IgG secondary antibodies; Related news: Cholesterol, the weakness of anaplastic large cell lymphoma (ALCL) |
| Research Area | Cancer antibody; Controls and Markers antibody; Metabolism antibody; Signaling Transduction antibody |
| Calculated MW | 45 kDa |
| PTM | Succinylation at Lys-268, adjacent to a coenzyme A binding site. Desuccinylated by SIRT5 (By similarity). |
Images (2) Click the Picture to Zoom In
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ARG54705 anti-ACAT1 antibody WB image
Western blot: 30 µg of 1) HepG2, 2) SW480, 3) 293T, 4) MDAMB231, and 5) MEF lysate stained with ARG54705 anti-ACAT1 antibody at 1:500 dilution.
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ARG54705 anti-ACAT1 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human hepatocarcinoma tissue stained with ARG54705 anti-ACAT1 antibody.
Customer's Feedback
Good
Review for anti-ACAT1 antibody
Application：WB
Sample：Mouse brain
Sample Loading Amount：30 µg
Primary Antibody Dilution Factor：1:500
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
Excellent
Review for anti-ACAT1 antibody
Application：WB
Sample：Jurkat cell lysate
Sample Loading Amount：30 µg
Primary Antibody Dilution Factor：1:500
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
Specific References
PCSK9Qβ-003 Vaccine Attenuates Atherosclerosis in Apolipoprotein E-Deficient Mice.
WB / Mouse
