ARG54705

anti-ACAT1 antibody

anti-ACAT1 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse

Cancer antibody; Controls and Markers antibody; Metabolism antibody; Signaling Transduction antibody
nuts_pic
2
publication_link Publication1

Overview

Product Description Rabbit Polyclonal antibody recognizes ACAT1
Tested Reactivity Hu, Ms
Predict Reactivity Mk
Tested Application IHC-P, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name ACAT1
Antigen Species Human
Immunogen KLH-conjugated synthetic peptide corresponding to aa. 296-329 (C-terminus) of Human ACAT1 (NP_000010.1).
Conjugation Un-conjugated
Alternate Names EC 2.3.1.9; Acetoacetyl-CoA thiolase; MAT; T2; ACAT; THIL; Acetyl-CoA acetyltransferase, mitochondrial

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-PAssay-dependent
WB1:1000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Purification This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Buffer PBS and 0.09% (W/V) Sodium azide
Preservative 0.09% (W/V) Sodium azide
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 110446 Mouse ACAT1

GeneID: 38 Human ACAT1

Swiss-port # P24752 Human Acetyl-CoA acetyltransferase, mitochondrial

Swiss-port # Q8QZT1 Mouse Acetyl-CoA acetyltransferase, mitochondrial

Gene Symbol ACAT1
Gene Full Name acetyl-CoA acetyltransferase 1
Background This gene encodes a mitochondrially localized enzyme that catalyzes the reversible formation of acetoacetyl-CoA from two molecules of acetyl-CoA. Defects in this gene are associated with 3-ketothiolase deficiency, an inborn error of isoleucine catabolism characterized by urinary excretion of 2-methyl-3-hydroxybutyric acid, 2-methylacetoacetic acid, tiglylglycine, and butanone. [provided by RefSeq, Feb 2009]
Function Plays a major role in ketone body metabolism. [From Uniprot]
Cellular Localization Mitochondrion.
Highlight Related products:
ACAT1 antibodies; Anti-Rabbit IgG secondary antibodies;
Related news:
Cholesterol, the weakness of anaplastic large cell lymphoma (ALCL)
Research Area Cancer antibody; Controls and Markers antibody; Metabolism antibody; Signaling Transduction antibody
Calculated MW 45 kDa
PTM Succinylation at Lys-268, adjacent to a coenzyme A binding site. Desuccinylated by SIRT5 (By similarity).

Images (2) Click the Picture to Zoom In

  • ARG54705 anti-ACAT1 antibody WB image

    Western blot: 30 µg of 1) HepG2, 2) SW480, 3) 293T, 4) MDAMB231, and 5) MEF lysate stained with ARG54705 anti-ACAT1 antibody at 1:500 dilution.

  • ARG54705 anti-ACAT1 antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Human hepatocarcinoma tissue stained with ARG54705 anti-ACAT1 antibody.

Customer's Feedback

nuts_pic      Good

Review for anti-ACAT1 antibody

Application:WB

Sample:Mouse brain

Sample Loading Amount:30 µg

Primary Antibody Dilution Factor:1:500

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

nuts_pic      Excellent

Review for anti-ACAT1 antibody

Application:WB

Sample:Jurkat cell lysate

Sample Loading Amount:30 µg

Primary Antibody Dilution Factor:1:500

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

Specific References

PCSK9Qβ-003 Vaccine Attenuates Atherosclerosis in Apolipoprotein E-Deficient Mice.

WB / Mouse

Danyu Wu et al.
Cardiovasc Drugs Ther.,  (2020)

publication_link

 

hr_line