ARG30003
Tag Internal Control Antibody Duo (DDDK tag, beta Actin)
Controls and Markers antibody; Signaling Transduction antibody
Publication33
Component
| Cat No | Component Name | Host clonality | Reactivity | Application | Package |
|---|---|---|---|---|---|
| ARG62986 | anti-DDDDK tag antibody [F-tag-01] | Mouse mAb | Other | ICC/IF, WB | 50 μg |
| ARG62346 | anti-beta Actin antibody [BA3R] | Mouse mAb | Hu, Ms, Rat, Chk, Rb | ELISA, FACS, ICC/IF, IP, WB | 50 μg |
Overview
| Product Description | DDDk tag, also known as FLAG-tag, FLAG octapeptide, DDDDK tag or DYKDDDDK tag, is a polypeptide protein tag (DYKDDDDK, 1012 Da) that can be added to a protein N or C-terminus using recombinant DNA technology. It can be used for affinity chromatography, to isolate recombinant protein from protein complexes with multiple subunits. DDDk tag is also used to detect protein cellular localization by immunofluorescence or protein expression level by SDS PAGE. Actins are a family of six proteins in three groups in human and other vertebrates: alpha ( ACTC1, cardiac muscle 1), alpha 1 ( ACTA1, skeletal muscle) and 2 ( ACTA2, aortic smooth muscle), beta ( ACTB), gamma 1 ( ACTG1) and 2 ( ACTG2, enteric smooth muscle). Beta and gamma 1 are two non-muscle actin proteins. They function as the main components of microfilaments. Actins are highly-conserved. Beta-actin proteins from species as diverse as human, mouse, and chicken are identical. Human beta actin shares close to 90% identity with fungal homologs. Human beta actin is at least 93% identical with other members of human actin family. Such homology information among the actin members and across species is important for antibody selection and the interpretation of Western blot bands. Both beta and alpha actins have been used as loading controls in Western blot experiments. [Labmoe] To study tagged-protein function in cells, researchers could use not only antibody recognizes tag polypeptide but also should chose a suitable internal control to control the loading of samples or the location of the intracellular organelles. ARG3003 Tag Internal control Duos (DDDK tag, beta Actin), provides not only an antibody reacts DDDK tag but also includes an antibody detects beta actin as a control to make your result more convincing |
|---|---|
| Target Name | Tag Internal Control |
| Alternate Names | Tag Internal Control antibody; beta Actin antibody; DDDDK tag antibody |
Properties
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
|---|---|
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Gene Full Name | Antibody Duo for Tag Internal Control (DDDK tag, beta Actin) |
|---|---|
| Research Area | Controls and Markers antibody; Signaling Transduction antibody |
Images (7) Click the Picture to Zoom In
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ARG62346 anti-beta Actin antibody [BA3R] ICC/IF image
Immunofluorescence: 100% Methanol fixed (RT, 10 min) HeLa cells stained with ARG62346 anti-beta Actin antibody [BA3R] at 1:500 dilution. Left: primary antibody (green). Right: Merge (primary antibody and DAPI).
Secondary antibody: ARG55393 Goat anti-Mouse IgG (H+L) antibody (FITC)
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ARG62346 anti-beta Actin antibody [BA3R] WB image
Western Blot: 20 μg of (1) human, (2) mouse, (3) rat, and (4) rabbit tissue lysates stained with ARG62346 anti-Actin antibody [BA3R] at 1:1000 (1 μg/mL) dilution
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ARG62986 anti-DDDDK tag antibody [F-tag-01] WB image
Western blot: 1) Mock transfected 2) Flag-tagged protein expression plasmid transfected HeLa cell lysate stained with ARG62986 anti-DDDDK tag antibody [F-tag-01].
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Monoclonal antibody clone F-tag-01 ICC/IF image
Immunofluorescence: COS-7 cells transfected with DDDDK expression vector stained with clone F-tag-01 followed by incubation with Goat anti-mouse IgG1 Alexa Fluor-594 (red). A) Fusion nuclear protein; B) cell nuclei stained with DAPI (blue); C) merged figures - confirmation of nuclear localization of the fusion protein; cell nuclei stained with DAPI (blue)
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ARG62346 anti-beta Actin antibody [BA3R] WB image
Western blot: MCF-7, A549, H1299, HCT116, HepG2 and HUVEC cell lysates stained with ARG62346 anti-beta Actin antibody [BA3R] at 1:1000 dilution.
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ARG62346 anti-beta Actin antibody [BA3R] WB image
Western blot: Rat brain, Rat heart, Rat kidney, Rat stomach and Rat ovary lysates stained with ARG62346 anti-beta Actin antibody [BA3R] at 1:1000 dilution.
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ARG62346 anti-beta Actin antibody [BA3R] WB image
Western blot: 20 µg of HeLa, Mouse brain and Rat brain lysates stained with ARG62346 anti-beta Actin antibody [BA3R] at 1:10000 dilution.
Specific References
Inhibition of circRNA NGFR promotes ferroptosis in gallbladder carcinoma cells
ARG62346: WB / Human
Troxerutin dampened hypothalamic neuroinflammation via microglial IL-22/IL-22R1/IRF3 activation in dihydrotestosterone-induced polycystic ovary syndrome rats
ARG62346: WB / Rat, Mouse
Proteasome inhibitor bortezomib prevents proliferation and migration of pulmonary arterial smooth muscle cells
ARG62346: WB / Rat
Suppression of gastric cancer cell proliferation by miR-494-3p inhibitor-loaded engineered exosomes
ARG62346: WB / Human
Isorhamnetin induces cell cycle arrest and apoptosis by triggering DNA damage and regulating the AMPK/mTOR/p70S6K signaling pathway in doxorubicin-resistant breast cancer
ARG62346: WB / Human
ErbB4 affects Th1/Th17 cell differentiation and promotes psoriasis progression
ARG62346: WB / Human
Poria cocos Lanostane Triterpenoids Extract Promotes Collagen and Hyaluronic Acid Production in D-Galactose-Induced Aging Rats
ARG62346: WB / Human
Coordination of LMO7 with FAK Signaling Sustains Epithelial Integrity in Renal Epithelia Exposed to Osmotic Pressure
ARG62346: WB / Rat
Zuogui Wan slowed senescence of bone marrow mesenchymal stem cells by suppressing Wnt/β-catenin signaling
ARG62346: WB / Rat
Pterostilbene Interferes With Lipopolysaccharide-Induced Myocardial Injury Through Oxidative Stress and Inflammasome Pathways
ARG62346: WB / Mouse
miR-145 attenuates phenotypic transformation of aortic vascular smooth muscle cells to prevent aortic dissection
ARG62346: WB / Human, Rat
Apolipoprotein M promotes cholesterol uptake and efflux from mouse macrophages.
ARG62346: WB / Mouse
MicroRNA-145 Delay Plaque Erosion by Down-Regulating TGFβr Ii and Suppressing Autophagy of Vascular Smooth Muscle Cells.
ARG62346: WB, ICC/IF / Human
Design, synthesis, and biological evaluation of a novel dual peroxisome proliferator-activated receptor alpha/delta agonist for the treatment of diabetic kidney disease through anti-inflammatory mechanisms.
ARG62346: WB / Mouse
Inhibition of inflammatory response by α-lipoic acid in autoimmune thyroiditis mice.
ARG62346: WB / Mouse
Study of isolation and characterization of extracellular vesicles in senescent tumor cells induced by chemotherapeutics.
ARG62346: WB / Human
Mutation of Arginine 264 on ERα (Estrogen Receptor Alpha) Selectively Abrogates the Rapid Signaling of Estradiol in the Endothelium Without Altering Fertility.
ARG62346: WB / Mouse
MiR-107 confers chemoresistance to colorectal cancer by targeting calcium-binding protein 39.
ARG62346: WB / Human
SMARCB1 Promotes Ubiquitination and Degradation of NR4A3 via Direct Interaction Driven by ROS in Vascular Endothelial Cell Injury
ARG62346: WB / Human, Rat
Porcine placental peptides improve neuroblast proliferation and differentiationvia enhancement of TrkB and BDNF levels in D-glatacose-induced mouse aging model.
ARG62346: WB / Mouse
Negative regulation of miR-1275 by H3K27me3 is critical for glial induction of glioblastoma cells.
ARG62346: WB / Human
A Novel Synthetic Steroid of 2β,3α,5α-Trihydroxy-androst-6-one Alleviates the Loss of Rat Retinal Ganglion Cells Caused by Acute Intraocular Hypertension via Inhibiting the Inflammatory Activation of Microglia.
ARG62346: WB / Mouse
Dengue virus nonstructural protein 1 activates platelets via Toll-like receptor 4, leading to thrombocytopenia and hemorrhage.
ARG62346: WB / Human
Neuroprotective effect of Paeoniae Radix Rubra on hippocampal CA1 region of mice induced by transient focal cerebral ischemia via anti-gliosis and anti-oxidant activity.
ARG62346: WB / Mouse
Changes in the Blood-Brain Barrier Function Are Associated With Hippocampal Neuron Death in a Kainic Acid Mouse Model of Epilepsy.
ARG62346: WB / Mouse
TET1 exerts its anti-tumor functions via demethylating DACT2 and SFRP2 to antagonize Wnt/β-catenin signaling pathway in nasopharyngeal carcinoma cells.
ARG62346: WB / Human
The expression of C1 inhibitor (C1INH) in macrophages is upregulated by retinal pigment epithelial cells - implication in subretinal immune privilege in the aging eye.
ARG62346: WB / Mouse
3'-Hydroxy-4'-methoxy-β-methyl-β-nitrostyrene inhibits tumor growth through ROS generation and GSH depletion in lung cancer cells.
ARG62346: WB / Human
H3K9ac and HDAC2 Activity Are Involved in the Expression of Monocarboxylate Transporter 1 in Oligodendrocyte.
ARG62346: WB / Rat
Both decreased Akt expression and mTOR phosphorylation are related to decreased neuronal differentiation in the hippocampal alveus of aged mice.
ARG62346: WB / Mouse
Neuroprotective Properties of Panax notoginseng Saponins via Preventing Oxidative Stress Injury in SAMP8 Mice.
ARG62346: WB / Mouse
miR-4443 Participates in the Malignancy of Breast Cancer.
ARG62346: WB / Human
miR-222 induces Adriamycin resistance in breast cancer through PTEN/Akt/p27kip1 pathway.
ARG62346: WB / Human
