ARG30003

Tag Internal Control Antibody Duo (DDDK tag, beta Actin)

Controls and Markers antibody; Signaling Transduction antibody
publication_link Publication34

Component

Cat No Component Name Host clonality Reactivity Application Package
ARG62986 anti-DDDDK tag antibody [F-tag-01] Mouse mAb Other ICC/IF, WB 50 μg
ARG62346 anti-beta Actin antibody [BA3R] Mouse mAb Hu, Ms, Rat, Chk, Rb ELISA, FACS, ICC/IF, IP, WB 50 μg

Overview

Product Description DDDk tag, also known as FLAG-tag, FLAG octapeptide, DDDDK tag or DYKDDDDK tag, is a polypeptide protein tag (DYKDDDDK, 1012 Da) that can be added to a protein N or C-terminus using recombinant DNA technology. It can be used for affinity chromatography, to isolate recombinant protein from protein complexes with multiple subunits. DDDk tag is also used to detect protein cellular localization by immunofluorescence or protein expression level by SDS PAGE. Actins are a family of six proteins in three groups in human and other vertebrates: alpha ( ACTC1, cardiac muscle 1), alpha 1 ( ACTA1, skeletal muscle) and 2 ( ACTA2, aortic smooth muscle), beta ( ACTB), gamma 1 ( ACTG1) and 2 ( ACTG2, enteric smooth muscle). Beta and gamma 1 are two non-muscle actin proteins. They function as the main components of microfilaments. Actins are highly-conserved. Beta-actin proteins from species as diverse as human, mouse, and chicken are identical. Human beta actin shares close to 90% identity with fungal homologs. Human beta actin is at least 93% identical with other members of human actin family. Such homology information among the actin members and across species is important for antibody selection and the interpretation of Western blot bands. Both beta and alpha actins have been used as loading controls in Western blot experiments. [Labmoe] To study tagged-protein function in cells, researchers could use not only antibody recognizes tag polypeptide but also should chose a suitable internal control to control the loading of samples or the location of the intracellular organelles. ARG3003 Tag Internal control Duos (DDDK tag, beta Actin), provides not only an antibody reacts DDDK tag but also includes an antibody detects beta actin as a control to make your result more convincing
Target Name Tag Internal Control
Alternate Names Tag Internal Control antibody; beta Actin antibody; DDDDK tag antibody

Properties

Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Gene Full Name Antibody Duo for Tag Internal Control (DDDK tag, beta Actin)
Research Area Controls and Markers antibody; Signaling Transduction antibody

Images (7) Click the Picture to Zoom In

  • ARG62346 anti-beta Actin antibody [BA3R] ICC/IF image

    Immunofluorescence: 100% Methanol fixed (RT, 10 min) HeLa cells stained with ARG62346 anti-beta Actin antibody [BA3R] at 1:500 dilution. Left: primary antibody (green). Right: Merge (primary antibody and DAPI).

    Secondary antibody: ARG55393 Goat anti-Mouse IgG (H+L) antibody (FITC)

  • ARG62346 anti-beta Actin antibody [BA3R] WB image

    Western Blot: 20 μg of (1) human, (2) mouse, (3) rat, and (4) rabbit tissue lysates stained with ARG62346 anti-Actin antibody [BA3R] at 1:1000 (1 μg/mL) dilution

  • ARG62986 anti-DDDDK tag antibody [F-tag-01] WB image

    Western blot: 1) Mock transfected 2) Flag-tagged protein expression plasmid transfected HeLa cell lysate stained with ARG62986 anti-DDDDK tag antibody [F-tag-01].

  • Monoclonal antibody clone F-tag-01 ICC/IF image

    Immunofluorescence: COS-7 cells transfected with DDDDK expression vector stained with clone F-tag-01 followed by incubation with Goat anti-mouse IgG1 Alexa Fluor-594 (red). A) Fusion nuclear protein; B) cell nuclei stained with DAPI (blue); C) merged figures - confirmation of nuclear localization of the fusion protein; cell nuclei stained with DAPI (blue)

  • ARG62346 anti-beta Actin antibody [BA3R] WB image

    Western blot: MCF-7, A549, H1299, HCT116, HepG2 and HUVEC cell lysates stained with ARG62346 anti-beta Actin antibody [BA3R] at 1:1000 dilution.

  • ARG62346 anti-beta Actin antibody [BA3R] WB image

    Western blot: Rat brain, Rat heart, Rat kidney, Rat stomach and Rat ovary lysates stained with ARG62346 anti-beta Actin antibody [BA3R] at 1:1000 dilution.

  • ARG62346 anti-beta Actin antibody [BA3R] WB image

    Western blot: 20 µg of HeLa, Mouse brain and Rat brain lysates stained with ARG62346 anti-beta Actin antibody [BA3R] at 1:10000 dilution.

Specific References

Mesenchymal stem cells-derived exosomes alleviate senescence of retinal pigment epithelial cells by activating PI3K/AKT-Nrf2 signaling pathway in early diabetic retinopathy

ARG62346: WB / Human

Lifang Bai et al.
Experimental Cell Research,  (2024)

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Suppression of gastric cancer cell proliferation by miR-494-3p inhibitor-loaded engineered exosomes

ARG62346: WB / Human

Limin Zhang et al.
Heliyon.,  (2024)

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Proteasome inhibitor bortezomib prevents proliferation and migration of pulmonary arterial smooth muscle cells

ARG62346: WB / Rat

Yi-Ching Liu et al.
Kaohsiung J Med Sci.,  (2024)

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Inhibition of circRNA NGFR promotes ferroptosis in gallbladder carcinoma cells

ARG62346: WB / Human

Desen Fan et al.
Heliyon.,  (2024)

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Troxerutin dampened hypothalamic neuroinflammation via microglial IL-22/IL-22R1/IRF3 activation in dihydrotestosterone-induced polycystic ovary syndrome rats

ARG62346: WB / Rat, Mouse

Zixuan Gao et al.
Phytomedicine.,  (2024)

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Isorhamnetin induces cell cycle arrest and apoptosis by triggering DNA damage and regulating the AMPK/mTOR/p70S6K signaling pathway in doxorubicin-resistant breast cancer

ARG62346: WB / Human

Tianshu Yang et al.
Phytomedicine.,  (2023)

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ErbB4 affects Th1/Th17 cell differentiation and promotes psoriasis progression

ARG62346: WB / Human

Rongrong Tang et al.
Cell Mol Biol (Noisy-le-grand).,  (2023)

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Poria cocos Lanostane Triterpenoids Extract Promotes Collagen and Hyaluronic Acid Production in D-Galactose-Induced Aging Rats

ARG62346: WB / Human

Chien-Liang Chao et al.
Life (Basel).,  (2023)

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Zuogui Wan slowed senescence of bone marrow mesenchymal stem cells by suppressing Wnt/β-catenin signaling

ARG62346: WB / Rat

Xiangping Kang et al.
J Ethnopharmacol.,  (2022)

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Pterostilbene Interferes With Lipopolysaccharide-Induced Myocardial Injury Through Oxidative Stress and Inflammasome Pathways

ARG62346: WB / Mouse

Lei Zhang et al.
Front Physiol .,  (2022)

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Coordination of LMO7 with FAK Signaling Sustains Epithelial Integrity in Renal Epithelia Exposed to Osmotic Pressure

ARG62346: WB / Rat

Yen-Yi Zhen et al.
cells,  (2022)

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Apolipoprotein M promotes cholesterol uptake and efflux from mouse macrophages.

ARG62346: WB / Mouse

Shuang Yao et al.
FEBS Open Bio.,  (2021)

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miR-145 attenuates phenotypic transformation of aortic vascular smooth muscle cells to prevent aortic dissection

ARG62346: WB / Human, Rat

Zhi-Huang Qiu et al.
J Clin Lab Anal.,  (2021)

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MicroRNA-145 Delay Plaque Erosion by Down-Regulating TGFβr Ii and Suppressing Autophagy of Vascular Smooth Muscle Cells.

ARG62346: WB, ICC/IF / Human

Yunjuan Jiao et al.
Preprint.,  (2021)

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Design, synthesis, and biological evaluation of a novel dual peroxisome proliferator-activated receptor alpha/delta agonist for the treatment of diabetic kidney disease through anti-inflammatory mechanisms.

ARG62346: WB / Mouse

Kai Liu et al.
Eur J Med Chem.,  (2021)

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Inhibition of inflammatory response by α-lipoic acid in autoimmune thyroiditis mice.

ARG62346: WB / Mouse

Mayinu Yusufu et al.
Basic & Clinical Medicine.,  (2021)

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Study of isolation and characterization of extracellular vesicles in senescent tumor cells induced by chemotherapeutics.

ARG62346: WB / Human

Qi Zhou et al.
Chinese Journal of Immunology.,  (2020)

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Mutation of Arginine 264 on ERα (Estrogen Receptor Alpha) Selectively Abrogates the Rapid Signaling of Estradiol in the Endothelium Without Altering Fertility.

ARG62346: WB / Mouse

Marine Adlanmerini et al.
Arterioscler Thromb Vasc Biol.,  (2020)

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MiR-107 confers chemoresistance to colorectal cancer by targeting calcium-binding protein 39.

ARG62346: WB / Human

Liang Yu et al.
Br J Cancer.,  (2020)

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SMARCB1 Promotes Ubiquitination and Degradation of NR4A3 via Direct Interaction Driven by ROS in Vascular Endothelial Cell Injury

ARG62346: WB / Human, Rat

Bingzheng Lu et al.
Oxid Med Cell Longev.,  (2020)

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Porcine placental peptides improve neuroblast proliferation and differentiationvia enhancement of TrkB and BDNF levels in D-glatacose-induced mouse aging model.

ARG62346: WB / Mouse

Quan Xu et al.
Journal of Chinese Pharmaceutical Sciences.,  (2019)

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Negative regulation of miR-1275 by H3K27me3 is critical for glial induction of glioblastoma cells.

ARG62346: WB / Human

Mai J et al.
Mol Oncol.,  (2019)

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A Novel Synthetic Steroid of 2β,3α,5α-Trihydroxy-androst-6-one Alleviates the Loss of Rat Retinal Ganglion Cells Caused by Acute Intraocular Hypertension via Inhibiting the Inflammatory Activation of Microglia.

ARG62346: WB / Mouse

Sun Hong-Jia-Qi et al.
Molecules.,  (2019)

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Dengue virus nonstructural protein 1 activates platelets via Toll-like receptor 4, leading to thrombocytopenia and hemorrhage.

ARG62346: WB / Human

Chao Chiao-Hsuan et al.
PLoS Pathog.,  (2019)

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Changes in the Blood-Brain Barrier Function Are Associated With Hippocampal Neuron Death in a Kainic Acid Mouse Model of Epilepsy.

ARG62346: WB / Mouse

Yan Bing-Chun et al.
Front Neurol.,  (2018)

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TET1 exerts its anti-tumor functions via demethylating DACT2 and SFRP2 to antagonize Wnt/β-catenin signaling pathway in nasopharyngeal carcinoma cells.

ARG62346: WB / Human

Fan Jiangxia et al.
Clin Epigenetics.,  (2018)

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The expression of C1 inhibitor (C1INH) in macrophages is upregulated by retinal pigment epithelial cells - implication in subretinal immune privilege in the aging eye.

ARG62346: WB / Mouse

Luo C et al.
Aging (Albany NY).,  (2018)

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Neuroprotective effect of Paeoniae Radix Rubra on hippocampal CA1 region of mice induced by transient focal cerebral ischemia via anti-gliosis and anti-oxidant activity.

ARG62346: WB / Mouse

Zhu Xiaolu et al.
Chinese Herbal Medicines.,  (2018)

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3'-Hydroxy-4'-methoxy-β-methyl-β-nitrostyrene inhibits tumor growth through ROS generation and GSH depletion in lung cancer cells.

ARG62346: WB / Human

Tsai CH et al.
Life Sci.,  (2017)

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H3K9ac and HDAC2 Activity Are Involved in the Expression of Monocarboxylate Transporter 1 in Oligodendrocyte.

ARG62346: WB / Rat

Lai Qing-Wei et al.
Front Mol Neurosci.,  (2017)

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Both decreased Akt expression and mTOR phosphorylation are related to decreased neuronal differentiation in the hippocampal alveus of aged mice.

ARG62346: WB / Mouse

Yan Bing-Chun et al.
Aging Clin Exp Res.,  (2017)

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Neuroprotective Properties of Panax notoginseng Saponins via Preventing Oxidative Stress Injury in SAMP8 Mice.

ARG62346: WB / Mouse

Huang JL et al.
Evidence-Based Complementary and Alternative Medicine,  (2017)

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miR-4443 Participates in the Malignancy of Breast Cancer.

ARG62346: WB / Human

Chen X et al.
PLoS One.,  (2016)

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miR-222 induces Adriamycin resistance in breast cancer through PTEN/Akt/p27kip1 pathway.

ARG62346: WB / Human

Wang DD et al.
Tumour Biol.,  (2016)

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