ARG62986
anti-DDDDK tag antibody [F-tag-01]
anti-DDDDK tag antibody [F-tag-01] for ICC/IF,Western blot and Other
Controls and Markers antibody; Tag Internal Control antibody
Overview
| Product Description | Mouse Monoclonal antibody [F-tag-01] recognizes DDDDK tag (Equivalent to Sigma's anti-FLAG antibodies). |
|---|---|
| Tested Reactivity | Other |
| Tested Application | ICC/IF, WB |
| Specificity | The clone F-tag-01 recognizes DDDDK-tagged proteins in all species. The small size of this tag and its high hydrophilicity decrease the probability of interference with its expression, proteolytic maturation, antigenicity, localization and function. |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | F-tag-01 |
| Isotype | IgG1 |
| Target Name | DDDDK tag |
| Antigen Species | Others |
| Immunogen | KLH-conjugated synthetic peptide: DYKDDDDK |
| Conjugation | Un-conjugated |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from hybridoma culture supernatant by protein-A affinity chromatography. |
| Purity | > 95% (by SDS-PAGE) |
| Buffer | PBS (pH 7.4) and 15 mM Sodium azide |
| Preservative | 15 mM Sodium azide |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Highlight | Related Antibody Duos and Panels: ARG30003 Tag Internal Control Antibody Duo (DDDK tag, beta Actin) Related products: DDDDK tag antibodies; DDDDK tag Duos / Panels; Anti-Mouse IgG secondary antibodies; |
|---|---|
| Research Area | Controls and Markers antibody; Tag Internal Control antibody |
Images (2) Click the Picture to Zoom In
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ARG62986 anti-DDDDK tag antibody [F-tag-01] ICC/IF image
Immunofluorescence: COS-7 cells transfected with DDDDK expression vector stained with ARG62986 anti-DDDDK tag antibody [F-tag-01] followed by incubation with Goat anti-mouse IgG1 Alexa Fluor-594 (red). A) Fusion nuclear protein; B) cell nuclei stained with DAPI (blue); C) merged figures.
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ARG62986 anti-DDDDK tag antibody [F-tag-01] WB image
Western blot: 1) Mock transfected 2) Flag-tagged protein expression plasmid transfected HeLa cell lysate stained with ARG62986 anti-DDDDK tag antibody [F-tag-01].
Clone References
Investigation of membrane protein-protein interactions using correlative FRET-PLA.
Influenza polymerase encoding mRNAs utilize atypical mRNA nuclear export.
Phosphorylation of Nanog is essential to regulate Bmi1 and promote tumorigenesis.
Proton-assisted amino acid transporter PAT1 complexes with Rag GTPases and activates TORC1 on late endosomal and lysosomal membranes.
ICC/IF /
Nuclear matrix binding site in the Rad51 recombinase.
WB, ICC/IF /
