ARG62346
anti-beta Actin antibody [BA3R]
anti-beta Actin antibody [BA3R] for ELISA,Flow cytometry,ICC/IF,Immunoprecipitation,Western blot and Human,Mouse,Rat,Chicken,Rabbit
Controls and Markers antibody; Signaling Transduction antibody; Loading Control antibody; Cytochrome-C fractionation Study antibody; Inflammation Study antibody; Tag Internal Control antibody
Overview
Product Description | Mouse Monoclonal antibody [BA3R] recognizes beta Actin |
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Tested Reactivity | Hu, Ms, Rat, Chk, Rb |
Tested Application | ELISA, FACS, ICC/IF, IP, WB |
Specificity | Recognizes native and denatured forms of β-Actin (around 42 kDa) |
Host | Mouse |
Clonality | Monoclonal |
Clone | BA3R |
Isotype | IgG2b |
Target Name | beta Actin |
Immunogen | β-Actin N-terminal peptide-KLH conjugates. |
Conjugation | Un-conjugated |
Alternate Names | PS1TP5BP1; BRWS1; Actin, cytoplasmic 1; Beta-actin |
Application Instructions
Application Suggestion |
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Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||||||
Positive Control | Mouse brain |
Properties
Form | Liquid |
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Purification | Protein A affinity purified. |
Purification Note | Protein A affinity chromatography from mouse ascites fluid. |
Buffer | 10mM PBS (pH 7.2) and 0.05% Sodium azide |
Preservative | 0.05% Sodium azide |
Concentration | 1 mg/ml |
Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
Database Links | |
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Gene Full Name | actin, beta |
Background | Beta actin is one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, integrity, and intercellular signaling. The encoded protein is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins that are ubiquitously expressed. Mutations in this gene cause Baraitser-Winter syndrome 1, which is characterized by intellectual disability with a distinctive facial appearance in human patients. Numerous pseudogenes of this gene have been identified throughout the human genome. [provided by RefSeq, Aug 2017] |
Function | Actin is a highly conserved protein that polymerizes to produce filaments that form cross-linked networks in the cytoplasm of cells (PubMed:29581253). Actin exists in both monomeric (G-actin) and polymeric (F-actin) forms, both forms playing key functions, such as cell motility and contraction (PubMed:29581253). In addition to their role in the cytoplasmic cytoskeleton, G- and F-actin also localize in the nucleus, and regulate gene transcription and motility and repair of damaged DNA (PubMed:29925947). [UniProt] |
Cellular Localization | Cytoplasm , cytoskeleton |
Highlight | Related Antibody Duos and Panels: ARG30003 Tag Internal Control Antibody Duo (DDDK tag, beta Actin) Related products: beta Actin antibodies; beta Actin Duos / Panels; Anti-Mouse IgG secondary antibodies; |
Research Area | Controls and Markers antibody; Signaling Transduction antibody; Loading Control antibody; Cytochrome-C fractionation Study antibody; Inflammation Study antibody; Tag Internal Control antibody |
Calculated MW | 42 kDa |
PTM | ISGylated. Oxidation of Met-44 and Met-47 by MICALs (MICAL1, MICAL2 or MICAL3) to form methionine sulfoxide promotes actin filament depolymerization. MICAL1 and MICAL2 produce the (R)-S-oxide form. The (R)-S-oxide form is reverted by MSRB1 and MSRB2, which promote actin repolymerization (By similarity). Monomethylation at Lys-84 (K84me1) regulates actin-myosin interaction and actomyosin-dependent processes. Demethylation by ALKBH4 is required for maintaining actomyosin dynamics supporting normal cleavage furrow ingression during cytokinesis and cell migration. (Microbial infection) Monomeric actin is cross-linked by V.cholerae toxins RtxA and VgrG1 in case of infection: bacterial toxins mediate the cross-link between Lys-50 of one monomer and Glu-270 of another actin monomer, resulting in formation of highly toxic actin oligomers that cause cell rounding (PubMed:19015515). The toxin can be highly efficient at very low concentrations by acting on formin homology family proteins: toxic actin oligomers bind with high affinity to formins and adversely affect both nucleation and elongation abilities of formins, causing their potent inhibition in both profilin-dependent and independent manners (PubMed:26228148). |
Images (4) Click the Picture to Zoom In
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ARG62346 anti-beta Actin antibody [BA3R] ICC/IF image
Immunofluorescence: 100% Methanol fixed (RT, 10 min) HeLa cells stained with ARG62346 anti-beta Actin antibody [BA3R] at 1:500 dilution. Left: primary antibody (green). Right: Merge (primary antibody and DAPI).
Secondary antibody: ARG55393 Goat anti-Mouse IgG (H+L) antibody (FITC)
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ARG62346 anti-beta Actin antibody [BA3R] WB image
Western blot: MCF-7, A549, H1299, HCT116, HepG2 and HUVEC cell lysates stained with ARG62346 anti-beta Actin antibody [BA3R] at 1:1000 dilution.
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ARG62346 anti-beta Actin antibody [BA3R] WB image
Western blot: Rat brain, Rat heart, Rat kidney, Rat stomach and Rat ovary lysates stained with ARG62346 anti-beta Actin antibody [BA3R] at 1:1000 dilution.
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ARG62346 anti-beta Actin antibody [BA3R] WB image
Western blot: 20 µg of HeLa, Mouse brain and Rat brain lysates stained with ARG62346 anti-beta Actin antibody [BA3R] at 1:10000 dilution.
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