ARG62346

anti-beta Actin antibody [BA3R]

anti-beta Actin antibody [BA3R] for ELISA,Flow cytometry,ICC/IF,Immunoprecipitation,Western blot and Human,Mouse,Rat,Chicken,Rabbit

Controls and Markers antibody; Signaling Transduction antibody; Loading Control antibody; Cytochrome-C fractionation Study antibody; Inflammation Study antibody; Tag Internal Control antibody
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Overview

Product Description Mouse Monoclonal antibody [BA3R] recognizes beta Actin
Tested Reactivity Hu, Ms, Rat, Chk, Rb
Tested Application ELISA, FACS, ICC/IF, IP, WB
Specificity Recognizes native and denatured forms of β-Actin (around 42 kDa)
Host Mouse
Clonality Monoclonal
Clone BA3R
Isotype IgG2b
Target Name beta Actin
Immunogen β-Actin N-terminal peptide-KLH conjugates.
Conjugation Un-conjugated
Alternate Names PS1TP5BP1; BRWS1; Actin, cytoplasmic 1; Beta-actin

Application Instructions

Application Suggestion
Tested Application Dilution
ELISAAssay-dependent
FACS0.1 - 1 µg/10^6 cells
ICC/IF1:500 - 1:2000
IP5 µg/ml
WB1:1000 - 1:10000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control Mouse brain

Properties

Form Liquid
Purification Protein A affinity purified.
Purification Note Protein A affinity chromatography from mouse ascites fluid.
Buffer 10mM PBS (pH 7.2) and 0.05% Sodium azide
Preservative 0.05% Sodium azide
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 100009272 Rabbit ACTB

GeneID: 11461 Mouse ACTB

GeneID: 396526 Chicken ACTB

Gene Full Name actin, beta
Background Beta actin is one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, integrity, and intercellular signaling. The encoded protein is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins that are ubiquitously expressed. Mutations in this gene cause Baraitser-Winter syndrome 1, which is characterized by intellectual disability with a distinctive facial appearance in human patients. Numerous pseudogenes of this gene have been identified throughout the human genome. [provided by RefSeq, Aug 2017]
Function Actin is a highly conserved protein that polymerizes to produce filaments that form cross-linked networks in the cytoplasm of cells (PubMed:29581253). Actin exists in both monomeric (G-actin) and polymeric (F-actin) forms, both forms playing key functions, such as cell motility and contraction (PubMed:29581253). In addition to their role in the cytoplasmic cytoskeleton, G- and F-actin also localize in the nucleus, and regulate gene transcription and motility and repair of damaged DNA (PubMed:29925947). [UniProt]
Cellular Localization Cytoplasm , cytoskeleton
Highlight Related Antibody Duos and Panels:
ARG30003 Tag Internal Control Antibody Duo (DDDK tag, beta Actin)
Related products:
beta Actin antibodies; beta Actin Duos / Panels; Anti-Mouse IgG secondary antibodies;
Research Area Controls and Markers antibody; Signaling Transduction antibody; Loading Control antibody; Cytochrome-C fractionation Study antibody; Inflammation Study antibody; Tag Internal Control antibody
Calculated MW 42 kDa
PTM ISGylated.
Oxidation of Met-44 and Met-47 by MICALs (MICAL1, MICAL2 or MICAL3) to form methionine sulfoxide promotes actin filament depolymerization. MICAL1 and MICAL2 produce the (R)-S-oxide form. The (R)-S-oxide form is reverted by MSRB1 and MSRB2, which promote actin repolymerization (By similarity).
Monomethylation at Lys-84 (K84me1) regulates actin-myosin interaction and actomyosin-dependent processes. Demethylation by ALKBH4 is required for maintaining actomyosin dynamics supporting normal cleavage furrow ingression during cytokinesis and cell migration.
(Microbial infection) Monomeric actin is cross-linked by V.cholerae toxins RtxA and VgrG1 in case of infection: bacterial toxins mediate the cross-link between Lys-50 of one monomer and Glu-270 of another actin monomer, resulting in formation of highly toxic actin oligomers that cause cell rounding (PubMed:19015515). The toxin can be highly efficient at very low concentrations by acting on formin homology family proteins: toxic actin oligomers bind with high affinity to formins and adversely affect both nucleation and elongation abilities of formins, causing their potent inhibition in both profilin-dependent and independent manners (PubMed:26228148).

Images (4) Click the Picture to Zoom In

  • ARG62346 anti-beta Actin antibody [BA3R] ICC/IF image

    Immunofluorescence: 100% Methanol fixed (RT, 10 min) HeLa cells stained with ARG62346 anti-beta Actin antibody [BA3R] at 1:500 dilution. Left: primary antibody (green). Right: Merge (primary antibody and DAPI).

    Secondary antibody: ARG55393 Goat anti-Mouse IgG (H+L) antibody (FITC)

  • ARG62346 anti-beta Actin antibody [BA3R] WB image

    Western blot: MCF-7, A549, H1299, HCT116, HepG2 and HUVEC cell lysates stained with ARG62346 anti-beta Actin antibody [BA3R] at 1:1000 dilution.

  • ARG62346 anti-beta Actin antibody [BA3R] WB image

    Western blot: Rat brain, Rat heart, Rat kidney, Rat stomach and Rat ovary lysates stained with ARG62346 anti-beta Actin antibody [BA3R] at 1:1000 dilution.

  • ARG62346 anti-beta Actin antibody [BA3R] WB image

    Western blot: 20 µg of HeLa, Mouse brain and Rat brain lysates stained with ARG62346 anti-beta Actin antibody [BA3R] at 1:10000 dilution.

Customer's Feedback

nuts_pic      Excellent

Review for anti-beta Actin antibody [BA3R]

Application:WB

Sample:HeLa, Mouse brain and Rat brain

Sample Loading Amount:20 µg

Primary Antibody Dilution Factor:1:10000

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

nuts_pic      Excellent

Review for anti-beta Actin antibody [BA3R]

Application:WB

Sample:MDA-MB-231 cells

Sample Loading Amount:20 µg

Primary Antibody Dilution Factor:0.2 μg/ml

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

nuts_pic      Excellent

Review for anti-beta Actin antibody [BA3R]

Application:WB

Sample:HaCaT cell

Sample Loading Amount:40μg

Primary Antibody Dilution Factor:1:10,000

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

Specific References

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Zixuan Gao et al.
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Chien-Liang Chao et al.
Life (Basel).,  (2023)

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Zhaowei Feng et al.
Biomed Pharmacother.,  (2023)

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Tianshu Yang et al.
Phytomedicine.,  (2023)

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Rongrong Tang et al.
Cell Mol Biol (Noisy-le-grand).,  (2023)

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Zuogui Wan slowed senescence of bone marrow mesenchymal stem cells by suppressing Wnt/β-catenin signaling

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Xiangping Kang et al.
J Ethnopharmacol.,  (2022)

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Pterostilbene Interferes With Lipopolysaccharide-Induced Myocardial Injury Through Oxidative Stress and Inflammasome Pathways

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Lei Zhang et al.
Front Physiol .,  (2022)

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Coordination of LMO7 with FAK Signaling Sustains Epithelial Integrity in Renal Epithelia Exposed to Osmotic Pressure

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Yen-Yi Zhen et al.
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Shuang Yao et al.
FEBS Open Bio.,  (2021)

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miR-145 attenuates phenotypic transformation of aortic vascular smooth muscle cells to prevent aortic dissection

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Zhi-Huang Qiu et al.
J Clin Lab Anal.,  (2021)

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MicroRNA-145 Delay Plaque Erosion by Down-Regulating TGFβr Ii and Suppressing Autophagy of Vascular Smooth Muscle Cells.

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Eur J Med Chem.,  (2021)

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Basic & Clinical Medicine.,  (2021)

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Qi Zhou et al.
Chinese Journal of Immunology.,  (2020)

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Marine Adlanmerini et al.
Arterioscler Thromb Vasc Biol.,  (2020)

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Br J Cancer.,  (2020)

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Bingzheng Lu et al.
Oxid Med Cell Longev.,  (2020)

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Quan Xu et al.
Journal of Chinese Pharmaceutical Sciences.,  (2019)

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Mai J et al.
Mol Oncol.,  (2019)

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Sun Hong-Jia-Qi et al.
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