ARG30309
Polycomb Complexes Antibody Panel (H3K27me3, EZH2, Ring1A, RNF2)
Component
| Cat No | Component Name | Host clonality | Reactivity | Application | Package |
|---|---|---|---|---|---|
| ARG54703 | anti-EZH2 antibody | Rabbit pAb | Hu, Ms | FACS, ICC/IF, IHC, WB | 50 μl |
| ARG63505 | anti-RNF2 / RING1B / BAP1 antibody | Goat pAb | Hu | WB | 50 μg |
| ARG51437 | anti-Ring1A antibody | Rabbit pAb | Hu, Ms, Rat | WB | 50 μl |
| ARG51851 | anti-Histone H3 trimethyl (Lys27) antibody | Rabbit pAb | Hu, Ms, Rat | ICC/IF, IHC-P, WB | 50 μl |
| ARG65351 | Goat anti-Rabbit IgG antibody (HRP) | Goat pAb | Rb | ELISA, IHC-P, WB | 50 μl |
| ARG65352 | Donkey anti-Goat IgG antibody (HRP) | Donkey pAb | Goat | ELISA, IHC, WB | 50 μl |
Overview
| Product Description | Polycomb-group (PcG) genes encode chromatin proteins involved in stable and heritable transcriptional silencing. PcG proteins participate in distinct multimeric complexes that deposit, or bind to, specific histone modifications (e.g., H3K27me3) to prevent gene activation and maintain repressed chromatin domains. Ezh2, Ring1A and Ring1B are core components of the mammalian Polycomb system, which regulates cell differentiation during embryonic development and tissue maintenance during adult life. Grossniklaus and Paro. Cold Spring Harb Perspect Biol 3:11 (2014) Rajasekhar and Begemann. Stem Cells 25: 2498-2510 (2007) Chen et al. Am J Transl Res: 4: 364-75 (2012) |
|---|---|
| Target Name | Polycomb Complexes |
| Alternate Names | Polycomb Complexes antibody; Ring1A antibody; Histone H3 trimethyl (Lys27) antibody; EZH2 antibody; RNF2 / RING1B / BAP1 antibody |
Properties
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
|---|---|
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Images (10) Click the Picture to Zoom In
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ARG54703 anti-EZH2 antibody WB image
Western blot: 30 µg of U2OS, Jurkat and PC3 cell lysates stained with ARG54703 anti-EZH2 antibody at 1:1000 dilution
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ARG54703 anti-EZH2 antibody WB image
Western blot: 30 µg of SW480 and HCT116 cell lysates stained with ARG54703 anti-EZH2 antibody at 1:1000 dilution.
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ARG63505 anti-RNF2 / RING1B / BAP1 antibody WB image
Western blot: 35 µg of K562 lysate stained with ARG63505 anti-RNF2 / RING1B / BAP1 antibody at 0.5 µg/ml dilution.
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ARG51437 anti-Ring1A antibody WB image
Western Blot: extract from HeLa cells stained with anti-Ring1A antibody ARG51437
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ARG51851 anti-Histone H3 (Tri-Methyl-Lys27) antibody WB image
Western Blot: extracts from HT29 and C6 cells stained with anti-Histone H3 (Tri-Methyl-Lys27) antibody ARG51851.
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ARG54703 anti-EZH2 antibody ICC/IF image
Immunofluorescence: 293 cells stained with ARG54703 anti-EZH2 antibody. Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).
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ARG54703 anti-EZH2 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human testis tissue stained with ARG54703 anti-EZH2 antibody.
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ARG51851 anti-Histone H3 (Tri-Methyl-Lys27) antibody IHC-P image
Immunohistochemistry: paraffin-embedded human breast carcinoma tissue stained with anti-Histone H3 (Tri-Methyl-Lys27) antibody ARG51851.
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ARG51851 anti-Histone H3 (Tri-Methyl-Lys27) antibody ICC/IF image
Immunofluorescence: methanol-fixed MEF cells stained with anti-Histone H3 (Tri-Methyl-Lys27) antibody ARG51851.
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ARG54703 anti-EZH2 antibody FACS image
Flow Cytometry: HeLa cells stained with ARG54703 anti-EZH2 antibody (right histogram) at 1:25 dilution or isotype control antibody (left histogram), followed by incubation with Alexa Fluor® 488 labelled secondary antibody.
