Overview

Product Description	Rabbit Polyclonal antibody recognizes Ring1A
Tested Reactivity	Hu, Ms, Rat
Tested Application	WB
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Target Name	Ring1A
Antigen Species	Human
Immunogen	Peptide sequence around aa.35~39( I-A-V-S-P) derived from Human Ring1A.
Conjugation	Un-conjugated
Alternate Names	RING finger protein 1; Polycomb complex protein RING1; EC 6.3.2.-; Really interesting new gene 1 protein; RING1A; RNF1; E3 ubiquitin-protein ligase RING1

Application Instructions

Application Suggestion	Tested Application Dilution WB 1:500 - 1:1000
Application Note	* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form	Liquid
Purification	Antibodies were produced by immunizing rabbits with KLH-conjugated synthetic peptide. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Buffer	PBS (without Mg2+ and Ca2+, pH 7.4), 150mM NaCl, 0.02% Sodium azide and 50% Glycerol.
Preservative	0.02% Sodium azide
Stabilizer	50% Glycerol
Concentration	1 mg/ml
Storage Instruction	For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note	For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links	GeneID: 19763 Mouse RING1 GeneID: 309626 Rat RING1 GeneID: 6015 Human RING1 Swiss-port # O35730 Mouse E3 ubiquitin-protein ligase RING1 Swiss-port # Q06587 Human E3 ubiquitin-protein ligase RING1 Swiss-port # Q6MGB6 Rat E3 ubiquitin-protein ligase RING1 More
Gene Symbol	RING1
Gene Full Name	ring finger protein 1
Background	Constitutes one of the E3 ubiquitin-protein ligases that mediate monoubiquitination of 'Lys-119' of histone H2A, thereby playing a central role in histone code and gene regulation. H2A 'Lys-119' ubiquitination gives a specific tag for epigenetic transcriptional repression and participates in X chromosome inactivation of female mammals. Essential component of the Polycomb group (PcG) multiprotein PRC1 complex, a complex required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex act via chromatin remodeling and modification of histones, rendering chromatin heritably changed in its expressibility. Compared to RNF2/RING2, it does not have the main E3 ubiquitin ligase activity on histone H2A, and it may rather act as a modulator of RNF2/RING2 activity.
Function	Constitutes one of the E3 ubiquitin-protein ligases that mediate monoubiquitination of 'Lys-119' of histone H2A, thereby playing a central role in histone code and gene regulation. H2A 'Lys-119' ubiquitination gives a specific tag for epigenetic transcriptional repression and participates in X chromosome inactivation of female mammals. Essential component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones, rendering chromatin heritably changed in its expressibility. Compared to RNF2/RING2, it does not have the main E3 ubiquitin ligase activity on histone H2A, and it may rather act as a modulator of RNF2/RING2 activity. [UniProt]
Highlight	Related Antibody Duos and Panels: ARG30309 Polycomb Complexes Antibody Panel (H3K27me3, EZH2, Ring1A, RNF2) Related products: Ring1A antibodies; Ring1A Duos / Panels; Anti-Rabbit IgG secondary antibodies;
Research Area	Gene Regulation antibody; Polycomb Complexes antibody
Calculated MW	42 kDa

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