ARG63922
anti-EP4 prostaglandin Receptor antibody
anti-EP4 prostaglandin Receptor antibody for Western blot and Human
Signaling Transduction antibody
Publication1
Overview
| Product Description | Goat Polyclonal antibody recognizes EP4 prostaglandin Receptor |
|---|---|
| Tested Reactivity | Hu |
| Predict Reactivity | Dog |
| Tested Application | WB |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | EP4 prostaglandin Receptor |
| Antigen Species | Human |
| Immunogen | C-LEREVSKNPDLQA |
| Conjugation | Un-conjugated |
| Alternate Names | PGE2 receptor EP4 subtype; Prostanoid EP4 receptor; Prostaglandin E2 receptor EP4 subtype; EP4; EP4R; PGE receptor EP4 subtype |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|
| Application Note | WB: Recommend incubate at RT for 1h. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from goat serum by antigen affinity chromatography. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P35408 Human Prostaglandin E2 receptor EP4 subtype |
|---|---|
| Background | The protein encoded by this gene is a member of the G-protein coupled receptor family. This protein is one of four receptors identified for prostaglandin E2 (PGE2). This receptor can activate T-cell factor signaling. It has been shown to mediate PGE2 induced expression of early growth response 1 (EGR1), regulate the level and stability of cyclooxygenase-2 mRNA, and lead to the phosphorylation of glycogen synthase kinase-3. Knockout studies in mice suggest that this receptor may be involved in the neonatal adaptation of circulatory system, osteoporosis, as well as initiation of skin immune responses. [provided by RefSeq, Jul 2008] |
| Research Area | Signaling Transduction antibody |
| Calculated MW | 53 kDa |
| PTM | Phosphorylation mediates agonist-mediated desensitization by promoting cytoplasmic retention. |
Images (2) Click the Picture to Zoom In
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ARG63922 anti-EP4 prostaglandin Receptor antibody WB image
Western blot: Endometrial Stromal cells stained with ARG63922 anti-EP4 prostaglandin Receptor antibody at 1:1000 dilution and ARG65352 Donkey anti-Goat IgG antibody (HRP).
From Qingqing Huang et al. Reprod Med Biol. (2021), doi: 10.1002/rmb2.12423, Fig. 3.C.
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ARG63922 anti-EP4 prostaglandin Receptor antibody WB image
Western blot: Human Testes lysate (35 µg protein in RIPA buffer) stained with ARG63922 anti-EP4 prostaglandin Receptor antibody at 0.1 µg/ml dilution.
Specific References
Higher fibrotic content of endometriotic lesions is associated with diminished prostaglandin E2 signaling
WB / Human
