ARG30267
Organelle Marker, Cytoplasm, Nucleus Antibody Duo (Histone, GAPDH)
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Component
Cat No | Component Name | Host clonality | Reactivity | Application | Package |
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ARG65681 | anti-Histone H3 antibody | Mouse mAb | Hu, Ms, Rat | ICC/IF, IHC-P, IP, WB | 50 μg |
ARG10112 | anti-GAPDH antibody [6C5] | Mouse mAb | Hu, Ms, Rat, AGMK, Bb, Cat, Chk, Dog, Fsh, Hm, Mk, Pig, Rb, Xenopus laevis, Zfsh | ELISA, ICC/IF, IHC-Fr, WB | 50 μg |
Overview
Product Description | GAPDH is often stably and constitutively expressed at high levels in most tissues and cells in cytoplasm. Therefore, GAPDH is a widely used loading control and cytoplasm marker for western blotting (35-40kDa) and Immunostaining study. |
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Target Name | Organelle Marker, Cytoplasm, Nucleus |
Alternate Names | Organelle Marker, Cytoplasm, Nucleus antibody; GAPDH antibody; Histone H3 antibody |
Properties
Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
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Note | For laboratory research only, not for drug, diagnostic or other use. |
Images (15) Click the Picture to Zoom In
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ARG10112 anti-GAPDH antibody [6C5] WB image
Western blot: 1) U87-MG 2) HepG2 3) rat brain 4) rat heart 5) rat ovary stained with ARG10112 anti-GAPDH antibody [6C5] at 1:2000 dilution.
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ARG10112 anti-GAPDH antibody [6C5] ICC/IF image
Immunofluorescence: 100% Methanol fixed (RT, 10 min) HeLa cells stained with ARG10112 anti-GAPDH antibody [6C5] (green) at 1:200 dilution.
Secondary antibody: ARG55393 Goat anti-Mouse IgG (H+L) antibody (FITC)
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ARG65681 anti-Histone H3 antibody ICC/IF image
Immunofluorescence: 100% Methanol fixed (RT, 10 min) HeLa cells stained with ARG65681 anti-Histone H3 antibody at 1:100 dilution. Left: primary antibody (green). Right: Merge (primary antibody and DAPI).
Secondary antibody: ARG55393 Goat anti-Mouse IgG (H+L) antibody (FITC)
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ARG65681 anti-Histone H3 antibody WB image
Western blot: 20 µg of HeLa, Mouse brain and Rat brain lysates stained with ARG65681 anti-Histone H3 antibody at 1:2000 dilution.
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ARG65681 anti-Histone H3 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Rat testis tissue stained with ARG65681 anti-Histone H3 antibody at 1:200 dilution (4°C, overnight). Antigen Retrieval: Boil tissue section in Sodium citrate buffer (pH 6.0) for 20 min. Secondary antibody was diluted at 1:200 (RT, 30 min).
Negative control was used by secondary antibody only.
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ARG65681 anti-Histone H3 antibody IP image
Immunoprecipitation: 1) HeLa cell lysate stained with ARG65681 anti-Histone H3 antibody and 2) IP product immunoprecipitated by ARG65681 anti-Histone H3 antibody at 1:200 dilution.
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ARG65681 anti-Histone H3 antibody WB image
Western blot: 1) HeLa, 2) Raw, 3) Mouse brain tissue, and 4) Rat brain tissue lysates stained with ARG65681 anti-Histone H3 antibody at 1:5000 dilution.
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ARG65681 anti-Histone H3 antibody IP image
Immunoprecipitation: 1) HeLa cell lysate stained with ARG65681 anti-Histone H3 antibody and 2) IP product immunoprecipitated by ARG65681 anti-Histone H3 antibody at 1:200 dilution.
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ARG10112 anti-GAPDH antibody [6C5] WB image
Western blot: 1) MCF-7 2) DU-145 3) A549 4) H1299 5) HCT116 6) HepG2 7) HUVEC stained with ARG10112 anti-GAPDH antibody [6C5] at 1:1000 dilution.
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ARG10112 anti-GAPDH antibody [6C5] WB image
Western blot: 1) U87-MG 2) MCF-7 3) A549 4) DU145 5) SW480 6) rat brain 7) rat stomach 8) rat ovary stained with ARG10112 anti-GAPDH antibody [6C5] at 1:5000 dilution.
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ARG10112 anti-GAPDH antibody [6C5] WB image
Western blot: 1) U87-MG 2) MCF-7 3) A549 4) DU145 5) SW480 6) rat brain 7) rat stomach 8) rat ovary stained with ARG10112 anti-GAPDH antibody [6C5] at 1:5000 dilution.
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ARG10112 anti-GAPDH antibody [6C5] WB image
Western blot: 1) U87-MG 2) HepG2 3) rat brain 4) rat heart 5) rat ovary stained with ARG10112 anti-GAPDH antibody [6C5] at 1:2000 dilution.
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ARG10112 anti-GAPDH antibody [6C5] WB image
Western Blot: 1) HeLa, 2) NTERA-2, 3) A-431, 4) HepG2, 5) MCF-7, 6) NIH 3T3, 7) PC-12 and 8) COS-7 whole cell lysates stained with anti-GAPDH antibody [6C5] (ARG10112)
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ARG10112 anti-GAPDH antibody [6C5] IP image
Immunoprecipitation and western blot: 1) GAPDH (1 μg). 2) GAPDH IP from rat heart tissue extract. 3) Only GAPDH preincubated with Protein A Sepharose. 4) Only Protein A Sepharose stained with ARG10112 GAPDH antibody [6C5].
Mixture of protein A-Sepharose with ARG10112 anti-GAPDH and tissue extract was incubated for 30 min at room temperature and precipitated by centrifugation. Pellet was washed with PBS, suspended in reducing electrophoresis sample buffer and heated for 5 minutes at 100ºC. After centrifugation supernatant was loaded on gel and proteins were separated by SDS electrophoresis. -
ARG10112 anti-GAPDH antibody [6C5] WB image
Western blot: 1) MCF-7 2) DU-145 3) A549 4) H1299 5) HCT116 6) HepG2 7) HUVEC stained with ARG10112 anti-GAPDH antibody [6C5] at 1:1000 dilution.
Specific References