ARG30267

Organelle Marker, Cytoplasm, Nucleus Antibody Duo (Histone, GAPDH)

Datasheet

Publication28

Ordering Information

Package

Price (USD)

1 pair

399.00

Add to cart

Datasheet
Publication (28)

Component

Cat No	Component Name	Host clonality	Reactivity	Application	Package
ARG65681	anti-Histone H3 antibody	Mouse mAb	Hu, Ms, Rat	ICC/IF, IHC-P, IP, WB	50 μg
ARG10112	anti-GAPDH antibody [6C5]	Mouse mAb	Hu, Ms, Rat, AGMK, Bb, Cat, Chk, Dog, Fsh, Hm, Mk, Pig, Rb, Xenopus laevis, Zfsh	ELISA, ICC/IF, IHC-Fr, WB	50 μg

Overview

Product Description	GAPDH is often stably and constitutively expressed at high levels in most tissues and cells in cytoplasm. Therefore, GAPDH is a widely used loading control and cytoplasm marker for western blotting (35-40kDa) and Immunostaining study. Histone H3 (15-20kDa) is one of the component of nucleosomes in nucleus. So Histone H3 is one of the commonly used loading control and nucleus marker in western blotting and Immunostaining study. This Cytoplasm, Nucleus antibody Duos not only provide different MW targets for internal control selection, but also provide cytoplasm (GAPDH) and nucleus (Histone H3) markers to control the quality of cytoplasm and nucleus fractions after subcellular fractionation process and to identify the cytoplasm and nucleus position in Immunostaining study.
Target Name	Organelle Marker, Cytoplasm, Nucleus
Alternate Names	Organelle Marker, Cytoplasm, Nucleus antibody; GAPDH antibody; Histone H3 antibody

Properties

Storage Instruction	For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note	For laboratory research only, not for drug, diagnostic or other use.

Images (18) Click the Picture to Zoom In

ARG10112 anti-GAPDH antibody [6C5] WB image

Western blot: 1) U87-MG 2) HepG2 3) rat brain 4) rat heart 5) rat ovary stained with ARG10112 anti-GAPDH antibody [6C5] at 1:2000 dilution.
ARG10112 anti-GAPDH antibody [6C5] ICC/IF image

Immunofluorescence: 100% Methanol fixed (RT, 10 min) HeLa cells stained with ARG10112 anti-GAPDH antibody [6C5] (green) at 1:200 dilution.

Secondary antibody: ARG55393 Goat anti-Mouse IgG (H+L) antibody (FITC)
ARG65681 anti-Histone H3 antibody ICC/IF image

Immunofluorescence: 100% Methanol fixed (RT, 10 min) HeLa cells stained with ARG65681 anti-Histone H3 antibody at 1:100 dilution. Left: primary antibody (green). Right: Merge (primary antibody and DAPI).

Secondary antibody: ARG55393 Goat anti-Mouse IgG (H+L) antibody (FITC)
ARG65681 anti-Histone H3 antibody WB image

Western blot: 20 µg of HeLa, Mouse brain and Rat brain lysates stained with ARG65681 anti-Histone H3 antibody at 1:2000 dilution.
ARG65681 anti-Histone H3 antibody IHC-P image

Immunohistochemistry: Paraffin-embedded Rat testis tissue stained with ARG65681 anti-Histone H3 antibody at 1:200 dilution (4°C, overnight). Antigen Retrieval: Boil tissue section in Sodium citrate buffer (pH 6.0) for 20 min. Secondary antibody was diluted at 1:200 (RT, 30 min).

Negative control was used by secondary antibody only.
ARG65681 anti-Histone H3 antibody IP image

Immunoprecipitation: 1) HeLa cell lysate stained with ARG65681 anti-Histone H3 antibody and 2) IP product immunoprecipitated by ARG65681 anti-Histone H3 antibody at 1:200 dilution.
ARG65681 anti-Histone H3 antibody WB image

Western blot: 1) HeLa, 2) Raw, 3) Mouse brain tissue, and 4) Rat brain tissue lysates stained with ARG65681 anti-Histone H3 antibody at 1:5000 dilution.
ARG65681 anti-Histone H3 antibody IP image

Immunoprecipitation: 1) HeLa cell lysate stained with ARG65681 anti-Histone H3 antibody and 2) IP product immunoprecipitated by ARG65681 anti-Histone H3 antibody at 1:200 dilution.
ARG10112 anti-GAPDH antibody [6C5] WB image

Western blot: 1) MCF-7 2) DU-145 3) A549 4) H1299 5) HCT116 6) HepG2 7) HUVEC stained with ARG10112 anti-GAPDH antibody [6C5] at 1:1000 dilution.
ARG10112 anti-GAPDH antibody [6C5] WB image

Western blot: 1) U87-MG 2) MCF-7 3) A549 4) DU145 5) SW480 6) rat brain 7) rat stomach 8) rat ovary stained with ARG10112 anti-GAPDH antibody [6C5] at 1:5000 dilution.
ARG10112 anti-GAPDH antibody [6C5] WB image

Western blot: 1) U87-MG 2) MCF-7 3) A549 4) DU145 5) SW480 6) rat brain 7) rat stomach 8) rat ovary stained with ARG10112 anti-GAPDH antibody [6C5] at 1:5000 dilution.
ARG10112 anti-GAPDH antibody [6C5] WB image

Western blot: 1) U87-MG 2) HepG2 3) rat brain 4) rat heart 5) rat ovary stained with ARG10112 anti-GAPDH antibody [6C5] at 1:2000 dilution.
ARG10112 anti-GAPDH antibody [6C5] WB image

Western blot: Mouse samples stained with ARG10112 anti-GAPDH antibody [6C5] at 1:1000 dilution.

From Yun-Yun Li et al. Int J Biol Sci (2022), doi: 10.7150/ijbs.68224, Fig. 5. C.
ARG10112 anti-GAPDH antibody [6C5] WB image

Western blot: Porcine kidney stained with ARG10112 anti-GAPDH antibody [6C5].

From Jianni Huang et al. Front Cell Dev Biol (2022), doi: 10.3389/fcell.2022.899869, Fig. 2. E.
ARG10112 anti-GAPDH antibody [6C5] WB image

Western blot: pAFs, AcoSV40, and AcoSI-1 stained with ARG10112 anti-GAPDH antibody [6C5] at 1:5000 dilution.

From Michele N Dill et al. PLoS One. (2023), doi: 10.3389/fcell.2022.899869, Fig. 2. C.
ARG10112 anti-GAPDH antibody [6C5] WB image

Western Blot: 1) HeLa, 2) NTERA-2, 3) A-431, 4) HepG2, 5) MCF-7, 6) NIH 3T3, 7) PC-12 and 8) COS-7 whole cell lysates stained with anti-GAPDH antibody [6C5] (ARG10112)
ARG10112 anti-GAPDH antibody [6C5] WB image

Western blot: 1) MCF-7 2) DU-145 3) A549 4) H1299 5) HCT116 6) HepG2 7) HUVEC stained with ARG10112 anti-GAPDH antibody [6C5] at 1:1000 dilution.
ARG10112 anti-GAPDH antibody [6C5] IP image

Immunoprecipitation and western blot: 1) GAPDH (1 μg). 2) GAPDH IP from rat heart tissue extract. 3) Only GAPDH preincubated with Protein A Sepharose. 4) Only Protein A Sepharose stained with ARG10112 GAPDH antibody [6C5].

Mixture of protein A-Sepharose with ARG10112 anti-GAPDH and tissue extract was incubated for 30 min at room temperature and precipitated by centrifugation. Pellet was washed with PBS, suspended in reducing electrophoresis sample buffer and heated for 5 minutes at 100ºC. After centrifugation supernatant was loaded on gel and proteins were separated by SDS electrophoresis.

Specific References

Shionone relieves oxygen-glucose deprivation/reoxygenation induced SH-SY5Y cells injury by inhibiting the p38 MAPK/NF-κB pathway

ARG10112: WB / Human

Xiaoli Zhou et al.
J Cardiothorac Surg, (2024)

7,8-Dihydroxyflavone ameliorates cognitive impairment induced by repeated neonatal sevoflurane exposures in mice through increasing tau O-GlcNAcylation

ARG10112: WB / Mouse

Mingliang Xu et al.
Neurosci Lett., (2024)

The effect of long-term administration of green tea catechins on aging-related cardiac diastolic dysfunction and decline of troponin I

ARG10112: WB / Mouse

Junjun Quan et al.
pre-print, (2024)

Biochanin A inhibits cardiac hypertrophy and fibrosis in vivo and in vitro

ARG10112: WB / Mouse, Rat

Zhenyu Feng et al.
Biomed Pharmacother., (2024)

PCSK6 exacerbates Alzheimer's disease pathogenesis by promoting MT5-MMP maturation

ARG10112: WB / Human, Mouse

Mingliang Xu et al.
Exp Neurol., (2024)

Uric acid mitigates cognitive deficits via transcription factor EB - mediated microglial autophagy in mice models of Alzheimer's disease

ARG10112: WB / Mouse

Qian Xiao et al.
, (2023)

黄芪甲苷预处理对大鼠肠缺血再灌注所致肺损伤的影响及其机制

ARG10112: WB / Rat

王蕾 et al.
中国中西医结合外科杂志, (2023)

PPARγ activation suppresses chondrocyte ferroptosis through mitophagy in osteoarthritis

ARG10112: WB / Rat

Xiang Xue et al.
J Orthop Surg Res., (2023)

Knockdown of Porf-2 restores visual function after optic nerve crush injury

ARG10112: WB / Mouse

Di Chen et al.
Cell Death Dis., (2023)

Discovery of the First Subnanomolar PPARα/δ Dual Agonist for the Treatment of Cholestatic Liver Diseases

ARG10112: WB / Mouse

Zhiqi Feng et al.
J Med Chem., (2023)

Resveratrol inhibits Toxoplasma gondii-induced lung injury, inflammatory cascade and evidences of its mechanism of action

ARG10112: WB / Mouse

Yu Nan Lu et al.
Phytomedicine., (2023)

Gut microbiome dysbiosis contributes to abdominal aortic aneurysm by promoting neutrophil extracellular trap formation

ARG65681: IHC-P / Mouse

Zhenyu Tian et al.
Cell Host Microbe, (2022)

SCM-198 Prevents Endometriosis by Reversing Low Autophagy of Endometrial Stromal Cell via Balancing ERα and PR Signals

ARG10112: WB / Mouse

Yi-Kong Lin et al.
Front Endocrinol (Lausanne), (2022)

Pharmacological inhibition of SMYD2 protects against cisplatin-induced acute kidney injury in mice

ARG10112: WB / Mouse

Binbin Cui et al.
Front Pharmacol, (2022)

Lack of interferon regulatory factor 3 leads to anxiety/depression-like behaviors through disrupting the balance of neuronal excitation and inhibition in mice

ARG10112: WB / Mouse

Junjie Li et al.
Genes & Diseases, (2022)

Mesenchymal Stem Cells-Derived Exosomes Ameliorate Ischemia/Reperfusion Induced Acute Kidney Injury in a Porcine Model

ARG10112: WB / Pig

Jianni Huang et al.
Frontiers in Cell and Developmental Biology, (2022)

Genetic labeling reveals cellular expression pattern of neuregulin 1 in mouse forebrain

ARG10112: WB / Human

Chen-Yun Ding et al.
Research square, (2022)

Downregulation of lncRNA NEAT1 alleviates sepsis-induced acute kidney injury

ARG10112: WB / Human

Yuhua Zhou et al.
Cent Eur J Immunol ., (2022)

Qi-Tai-Suan, an oleanolic acid derivative, ameliorates ischemic heart failure via suppression of cardiac apoptosis, inflammation and fibrosis

ARG10112: WB / Human

QIAN Ming et al.
Chinese Journal of Natural Medicines , (2022)

M351-0056 is a novel low MW compound modulating the actions of the immune-checkpoint protein VISTA.

ARG10112: WB / Human, Mouse

Xinlei Hu et al.
Br J Pharmacol., (2021)

7,8-dihydroxyflavone ameliorates motor deficits via regulating autophagy in MPTP-induced mouse model of Parkinson's disease

ARG10112: WB / Mouse

Li Zuo et al.
Cell Death Discov., (2021)

Cardiac-Specific Gene TNNI3 as a Potential Oncogene for Kidney Cancer and Its Involvement in Wnt Signaling Pathway.

ARG10112: WB / Human

Weian Zhao et al.
Preprint., (2021)

Mesenchymal Stem Cell-Derived Exosomes Ameliorate Alzheimer's Disease Pathology and Improve Cognitive Deficits.

ARG10112: WB / Human

Yi-An Chen et al.
Biomedicines., (2021)

Circ_0081572 inhibits the progression of periodontitis through regulating the miR-378h/RORA axis.

ARG10112: WB / Human

Jing Wang et al.
Arch Oral Biol., (2021)

Structural Basis of VSIG3: The Ligand for VISTA.

ARG10112: WB / Human

Xiaoxue Xie et al.
Front Immunol., (2021)

Evaluation of Class IIa Histone Deacetylases Expression and In Vivo Epigenetic Imaging in a Transgenic Mouse Model of Alzheimer's Disease

ARG10112: WB / Human

Yi-An Chen et al.
Int J Mol Sci., (2021)

The Phosphorylation Status of Drp1-Ser637 by PKA in Mitochondrial Fission Modulates Mitophagy via PINK1/Parkin to Exert Multipolar Spindles Assembly during Mitosis.

ARG10112: WB / Human

Huey-Jiun Ko et al.
Biomolecules., (2021)

Curcumin attenuates renal ischemia reperfusion injury via JNK pathway with the involvement of p300/CBP-mediated histone acetylation

ARG10112: WB / Rat

Lu Yang et al.
Korean J Physiol Pharmacol., (2021)