ARG30324
Neuroinflammation Antibody Panel
Publication9
Component
| Cat No | Component Name | Host clonality | Reactivity | Application | Package |
|---|---|---|---|---|---|
| ARG11062 | anti-AIF1 / Iba 1 antibody | Rabbit pAb | Hu, Ms, Rat | ICC/IF, IHC-Fr, WB | 20 μl |
| ARG10514 | anti-CD68 antibody [KP1] | Mouse mAb | Hu, Ms, Rat, Rb | FACS, ICC/IF, IHC-FoFr , IHC-Fr, IHC-P, WB | 20 μg |
| ARG52313 | anti-GFAP antibody | Chicken pAb | Hu, Ms, Rat, Bov, Cat, Chk, R. Mk, Rb | ELISA, FACS, ICC/IF, IHC-Fr, IHC-P, WB | 20 μl |
Overview
| Product Description | Neuroinflammation Antibody Panel is an all-in-one solution to make neuroinflammation research easy and economic. It is ideal for studying neuroinflammation in rodent models and patient samples. This antibody panel comprises the most popular neuroinflammation markers: the active macroglial marker CD68 antibody, macroglial marker Iba-1 antibody, and astrocyte marker GFAP antibody. All the antibodies in this panel have excellent staining performance. Moreover, the different host species of the antibodies make the panel as the best choice for triple staining. Related news: Inflammation antibody panels are released Exploring Antiviral Immune Response |
|---|---|
| Target Name | Neuroinflammation |
| Alternate Names | Neuroinflammation antibody; CD68 antibody; AIF1 / Iba 1 antibody; GFAP antibody |
Properties
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
|---|---|
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Gene Full Name | Antibody Panel for Neuroinflammation |
|---|---|
| Highlight | Related products: Inflammation antibodies; Inflammation Duos / Panels; |
Images (10) Click the Picture to Zoom In
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ARG11062 anti-AIF1 / Iba 1 antibody ICC/IF image (Customer's Feedback)
Immunofluorescence: BV-2 stained with ARG11062 anti-AIF1 / Iba 1 antibody at 1:100 dilution.
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ARG52313 anti-GFAP antibody WB image
Western Blot: Rat cortex lysate showing specific immunolabeling of the ~50k GFAP protein stained with ARG52313 anti-GFAP antibody.
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ARG11062 anti-AIF1 / Iba 1 antibody IHC-Fr image
Immunohistochemistry: High magnification stacked confocal image of Rat cerebellar molecular layer at top and granular layer below, stained with ARG11062 anti-AIF1 / Iba 1 antibody (green) at 1:1000 dilution. Nuclear DNA is shown with DAPI stain in blue.
Microglia are very small cells with fine processes spreading in three dimensions and so are best visualized in a confocal Z stack. Red shows the processes of Purkinje cells and the perikarya of granule cells revealed by an antibody to MAP2, at 1:5000 dilution.
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ARG10514 anti-CD68 antibody [KP1] IHC-P image
Immunohistochemistry: Formalin-fixed and paraffin-embedded Human tonsil stained with ARG10514 anti-CD68 antibody [KP1]. Antigen Retrieval: Boil tissue section in Citrate buffer (pH 6.0).
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ARG52313 anti-GFAP antibody ICC/IF image
Immunofluorescence: Mixed cultures of neurons and glia stained with ARG52313 anti-GFAP antibody (red), and DNA (blue). Astrocytes stain strongly and specifically in a clearly filamentous fashion with this antibody.
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ARG10514 anti-CD68 antibody [KP1] WB image
Western blot: Human Macrophages and N1 Fibroblast untreated or treated with N-glycosidase F. The blots were stained with ARG10514 anti-CD68 antibody [KP1].
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ARG11062 anti-AIF1 / Iba 1 antibody WB image
Western blot: Mouse brain, Rat brain, Mouse spleen, and Rat spleen lysates stained with ARG11062 anti-AIF1 / Iba 1 antibody at 1:1000 dilution.
Iba1 is a relatively minor protein of brain and is much more abundant in spleen, so the 15 kDa band is less obvious in CNS lysates. The other bands seen in the CNS lysates are of unknown origin but do not appear to compromise the migroglial specific staining seen with this antibody.
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ARG10514 anti-CD68 antibody [KP1] FACS image
Flow Cytometry: THP1 cells prefixed with 4% PFA and then permeabilised with 0.25% saponin. Cells were stained with ARG10514 anti-CD68 antibody [KP1] (white area) or isotype control antibody (gray area).
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ARG10514 anti-CD68 antibody [KP1] IHC-P image
Immunohistochemistry: Formalin-fixed and paraffin-embedded Human breast carcinoma stained with ARG10514 anti-CD68 antibody [KP1].
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ARG10514 anti-CD68 antibody [KP1] IHC-P image
Immunohistochemistry: Paraffin-embedded Mouse kidney stained with ARG10514 anti-CD68 antibody [KP1] at 1:200 dilution.
Secondary antibody: Anti-Mouse IgG antibody (Rhodamine red).
Specific References
Mac-1 deficiency ameliorates pressure overloaded heart failure through inhibiting macrophage polarization and activation
ARG10514: IHC-Fr / Mouse
Facile and rapid fabrication of a novel 3D-printable, visible light-crosslinkable and bioactive polythiourethane for large-to-massive rotator cuff tendon repair
ARG10514: IHC-P / Mouse
The Proinflammatory Role of Guanylate-Binding Protein 5 in Inflammatory Bowel Diseases
ARG10514: ICC-IF / Mouse
Complement-mediated M2/M1 macrophage polarization may be involved in crescent formation in lupus nephritis
ARG10514: IHC-P / Human
Btk knockout attenuates the liver inflammation in STZ-induced diabetic mice by suppressing NLRP3 inflammasome activation.
ARG10514: IHC-P / Mouse
Enhanced Bruton's tyrosine kinase activity in the kidney of patients with IgA nephropathy.
ARG10514: IHC-P / Human
TAB1 regulates glycolysis and activation of macrophages in diabetic nephropathy.
ARG10514: IHC-P / Mouse
Dark Sweet Cherry ( Prunus avium) Phenolics Enriched in Anthocyanins Induced Apoptosis in MDA-MB-453 Breast Cancer Cells through MAPK-Dependent Signaling and Reduced Invasion via Akt and PLCγ-1 Downregulation.
ARG11062: WB / Human
Exosomes from high glucose-treated macrophages activate glomerular mesangial cells via TGF-β1/Smad3 pathway in vivo and in vitro.
ARG10514: IHC-P / Mouse
