ARG11062
anti-AIF1 / Iba 1 antibody
anti-AIF1 / Iba 1 antibody for ICC/IF,IHC-Frozen sections,Western blot and Human,Mouse,Rat
Cell Biology and Cellular Response antibody; Immune System antibody; Metabolism antibody; Neuroscience antibody; Activated Macrophage/Microglia Study antibody; Neuroinflammation Study antibody; Macroglial Marker antibody
Publication1
Overview
| Product Description | Rabbit Polyclonal antibody recognizes AIF1 / Iba 1 |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | ICC/IF, IHC-Fr, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | AIF1 / Iba 1 |
| Antigen Species | Human |
| Immunogen | KLH-conjugated synthetic peptide around the C-terminal region of Human AIF1 / Iba1. |
| Conjugation | Un-conjugated |
| Alternate Names | Ionized calcium-binding adapter molecule 1; Allograft inflammatory factor 1; IBA1; IRT-1; IRT1; AIF-1; Protein G1 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| Observed Size | 17 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Unpurified |
| Buffer | Serum and 5mM Sodium azide. |
| Preservative | 5mM Sodium azide |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | AIF1 |
| Gene Full Name | allograft inflammatory factor 1 |
| Background | AIF1 / Iba 1 is a protein that binds actin and calcium. This gene is induced by cytokines and interferon and may promote macrophage activation and growth of vascular smooth muscle cells and T-lymphocytes. Polymorphisms in this gene may be associated with systemic sclerosis. Alternative splicing results in multiple transcript variants, but the full-length and coding nature of some of these variants is not certain. [provided by RefSeq, Jan 2016] |
| Function | AIF1 / Iba 1 is an Actin-binding protein. It enhances membrane ruffling and RAC activation. Enhances the actin-bundling activity of LCP1. Binds calcium. Plays a role in RAC signaling and in phagocytosis. May play a role in macrophage activation and function. Promotes the proliferation of vascular smooth muscle cells and of T-lymphocytes. Enhances lymphocyte migration. Plays a role in vascular inflammation. [UniProt] |
| Highlight | Related Antibody Duos and Panels: ARG30324 Neuroinflammation Antibody Panel Related products: AIF1 antibodies; AIF1 Duos / Panels; Anti-Rabbit IgG secondary antibodies; |
| Research Area | Cell Biology and Cellular Response antibody; Immune System antibody; Metabolism antibody; Neuroscience antibody; Activated Macrophage/Microglia Study antibody; Neuroinflammation Study antibody; Macroglial Marker antibody |
| Calculated MW | 17 kDa |
| PTM | Phosphorylated on serine residues. [UniProt] |
Images (3) Click the Picture to Zoom In
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ARG11062 anti-AIF1 / Iba 1 antibody ICC/IF image (Customer's Feedback)
Immunofluorescence: BV-2 stained with ARG11062 anti-AIF1 / Iba 1 antibody at 1:100 dilution.
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ARG11062 anti-AIF1 / Iba 1 antibody IHC-Fr image
Immunohistochemistry: High magnification stacked confocal image of Rat cerebellar molecular layer at top and granular layer below, stained with ARG11062 anti-AIF1 / Iba 1 antibody (green) at 1:1000 dilution. Nuclear DNA is shown with DAPI stain in blue.
Microglia are very small cells with fine processes spreading in three dimensions and so are best visualized in a confocal Z stack. Red shows the processes of Purkinje cells and the perikarya of granule cells revealed by an antibody to MAP2, at 1:5000 dilution.
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ARG11062 anti-AIF1 / Iba 1 antibody WB image
Western blot: Mouse brain, Rat brain, Mouse spleen, and Rat spleen lysates stained with ARG11062 anti-AIF1 / Iba 1 antibody at 1:1000 dilution.
Iba1 is a relatively minor protein of brain and is much more abundant in spleen, so the 15 kDa band is less obvious in CNS lysates. The other bands seen in the CNS lysates are of unknown origin but do not appear to compromise the migroglial specific staining seen with this antibody.
Customer's Feedback
Excellent
Review for anti-AIF1 / Iba 1 antibody
Application：IF/ICC
Sample：BV-2
Fixation Buffer：4% Paraformaldehyde
Fixation Time：20 min
Fixation Temperature：Room Temperature ºC
Primary Antibody Dilution Factor：1 : 100
Primary Antibody Incubation Time：Overnight
Primary Antibody Incubation Temperature：4 ºC
Excellent
Review for anti-AIF1 / Iba 1 antibody
Application：IF/ICC
Sample：BV-2
Fixation Buffer：4% Paraformaldehyde
Fixation Time：20 min
Fixation Temperature：Room Temperature ºC
Primary Antibody Dilution Factor：1 : 100
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
Specific References
Dark Sweet Cherry ( Prunus avium) Phenolics Enriched in Anthocyanins Induced Apoptosis in MDA-MB-453 Breast Cancer Cells through MAPK-Dependent Signaling and Reduced Invasion via Akt and PLCγ-1 Downregulation.
WB / Human
