ARG10514
anti-CD68 antibody [KP1]
anti-CD68 antibody [KP1] for Flow cytometry,ICC/IF,Immunohistochemistry (PFA perfusion fixed frozen sections),IHC-Frozen sections,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat,Rabbit
Immune System antibody; Activated Macrophage/Microglia Study antibody; Neuroinflammation Study antibody; Active macroglial Marker antibody; M1/M2/TAM Marker antibody; Macrophage Marker antibody; M1 macrophage Marker antibody; Inflammatory Cell Marker antibody
Publication10
Overview
| Product Description | Mouse Monoclonal antibody [KP1] recognizes CD68 |
|---|---|
| Tested Reactivity | Hu, Ms, Rat, Rb |
| Tested Application | FACS, ICC/IF, IHC-FoFr , IHC-Fr, IHC-P, WB |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | KP1 |
| Isotype | IgG1 |
| Target Name | CD68 |
| Immunogen | Lysosomal contents of lung macrophages. |
| Conjugation | Un-conjugated |
| Alternate Names | Macrosialin; CD antigen CD68; LAMP4; Gp110; GP110; SCARD1 |
Application Instructions
| Application Suggestion |
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| Application Note | IHC-P: Antigen Retrieval: Boil tissue section in Tris-EDTA pH9.0 or Citrate buffer (pH 6.0). Incubate with antibody at 4°C for overnight. IHC-Fr: Incubate with the antibody at 4°C for overnight or at RT for 30-60 mins. Flow cytometry: Membrane permeabilisation is required for this application. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| Observed Size | ~ 110 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified by affinity chromatography. |
| Buffer | PBS and 0.02% Sodium azide |
| Preservative | 0.02% Sodium azide |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | CD68 |
| Gene Full Name | CD68 molecule |
| Background | CD68 is a 110-kD transmembrane glycoprotein that is highly expressed by human monocytes and tissue macrophages. It is a member of the lysosomal/endosomal-associated membrane glycoprotein (LAMP) family. The protein primarily localizes to lysosomes and endosomes with a smaller fraction circulating to the cell surface. It is a type I integral membrane protein with a heavily glycosylated extracellular domain and binds to tissue- and organ-specific lectins or selectins. The protein is also a member of the scavenger receptor family. Scavenger receptors typically function to clear cellular debris, promote phagocytosis, and mediate the recruitment and activation of macrophages. Alternative splicing results in multiple transcripts encoding different isoforms. [provided by RefSeq, Jul 2008] |
| Function | CD68 could play a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. Binds to tissue- and organ-specific lectins or selectins, allowing homing of macrophage subsets to particular sites. Rapid recirculation of CD68 from endosomes and lysosomes to the plasma membrane may allow macrophages to crawl over selectin-bearing substrates or other cells. [UniProt] |
| Highlight | Related Antibody Duos and Panels: ARG30317 Activated Macrophage / Microglia Marker Antibody Duo ARG30324 Neuroinflammation Antibody Panel ARG30333 M1/M2/TAM Marker Antibody Panel Related products: CD68 antibodies; CD68 Duos / Panels; Anti-Mouse IgG secondary antibodies; Related news: Tumor-Infiltrating Lymphocytes (TILs) New antibody panels and duos for Tumor immune microenvironment Exploring Antiviral Immune Response Anti-SerpinB9 therapy, a new strategy for cancer therapy RIP1 activation and pathogenesis of NASH |
| Research Area | Immune System antibody; Activated Macrophage/Microglia Study antibody; Neuroinflammation Study antibody; Active macroglial Marker antibody; M1/M2/TAM Marker antibody; Macrophage Marker antibody; M1 macrophage Marker antibody; Inflammatory Cell Marker antibody |
| Calculated MW | 37 kDa |
| PTM | N- and O-glycosylated. |
Images (5) Click the Picture to Zoom In
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ARG10514 anti-CD68 antibody [KP1] IHC-P image
Immunohistochemistry: Formalin-fixed and paraffin-embedded Human tonsil stained with ARG10514 anti-CD68 antibody [KP1]. Antigen Retrieval: Boil tissue section in Citrate buffer (pH 6.0).
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ARG10514 anti-CD68 antibody [KP1] WB image
Western blot: Human Macrophages and N1 Fibroblast untreated or treated with N-glycosidase F. The blots were stained with ARG10514 anti-CD68 antibody [KP1].
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ARG10514 anti-CD68 antibody [KP1] FACS image
Flow Cytometry: THP1 cells prefixed with 4% PFA and then permeabilised with 0.25% saponin. Cells were stained with ARG10514 anti-CD68 antibody [KP1] (white area) or isotype control antibody (gray area).
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ARG10514 anti-CD68 antibody [KP1] IHC-P image
Immunohistochemistry: Formalin-fixed and paraffin-embedded Human breast carcinoma stained with ARG10514 anti-CD68 antibody [KP1].
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ARG10514 anti-CD68 antibody [KP1] IHC-P image
Immunohistochemistry: Paraffin-embedded Mouse kidney stained with ARG10514 anti-CD68 antibody [KP1] at 1:200 dilution.
Secondary antibody: Anti-Mouse IgG antibody (Rhodamine red).
Specific References
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IHC-P / Mouse
Mac-1 deficiency ameliorates pressure overloaded heart failure through inhibiting macrophage polarization and activation
IHC-Fr / Mouse
Farrerol prevents Angiotensin II-induced cardiac remodeling in vivo and in vitro
IHC-P / Mouse
Association of immune-related expression profile with sensitivity to chemotherapy in esophageal squamous cell carcinoma
IHC-P / Human
The Proinflammatory Role of Guanylate-Binding Protein 5 in Inflammatory Bowel Diseases
ICC-IF / Human
Complement-mediated M2/M1 macrophage polarization may be involved in crescent formation in lupus nephritis
IHC-P / Human
Btk knockout attenuates the liver inflammation in STZ-induced diabetic mice by suppressing NLRP3 inflammasome activation.
IHC-P / Mouse
Enhanced Bruton's tyrosine kinase activity in the kidney of patients with IgA nephropathy.
IHC-P / Human
TAB1 regulates glycolysis and activation of macrophages in diabetic nephropathy.
IHC-P / Mouse
Exosomes from high glucose-treated macrophages activate glomerular mesangial cells via TGF-β1/Smad3 pathway in vivo and in vitro.
IHC-P / Mouse
