ARG30251

NFkB nuclear translocation Antibody Panel

Datasheet

Publication22

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1 kit

815.00

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Datasheet
Publication (22)

Component

Cat No	Component Name	Host clonality	Reactivity	Application	Package
ARG10112	anti-GAPDH antibody [6C5]	Mouse mAb	Hu, Ms, Rat, AGMK, Bb, Cat, Chk, Dog, Fsh, Hm, Mk, Pig, Rb, Xenopus laevis, Zfsh	ELISA, ICC/IF, IHC-Fr, WB	50 μg
ARG54747	anti-Histone H3 antibody	Mouse mAb	Hu	WB	100 μl
ARG54746	anti-NFkB p105 / p50 antibody	Mouse mAb	Hu	FACS, IHC-P, WB	100 μl
ARG51013	anti-NFkB p65 antibody	Rabbit pAb	Hu, Ms, Rat	IHC-Fr, IHC-P, WB	50 μl
ARG65350	Goat anti-Mouse IgG antibody (HRP)	Goat pAb	Ms	ELISA, IHC-P, WB	50 μl

Overview

Product Description	Nuclear factor kappa B (NFkB) is a protein complex that controls large number of genes responsible for the regulation of apoptosis, inflammation, tumorigenesis and a variety of immune responses. NF-kB is sensitive and responsive to stimuli such as cytokines, stress, UV, low oxygen, bacterial or viral infections. Upon activation, NFkB is translocated into nucleus and exert its function on the transcriptional regulation of NFkB-responsive genes. Histone H3 is a marker for nuclear fraction while GAPDH is a marker for cytosolic fraction. Baeuerle et al. 1991. Biochem Biophys Acta 1072: 63-80 Lenardo and Baltimore. 1989. Cell 58: 227
Target Name	NFkB nuclear translocation
Alternate Names	NFkB nuclear translocation antibody; GAPDH antibody; NFkB p65 antibody; NFkB p105 / p50 antibody; Histone H3 antibody

Properties

Storage Instruction	For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note	For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Gene Full Name	Antibody Panel for NFkB nuclear translocation
Research Area	Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Controls and Markers antibody; Gene Regulation antibody; Immune System antibody; Metabolism antibody; Microbiology and Infectious Disease antibody; Neuroscience antibody; Signaling Transduction antibody

Images (24) Click the Picture to Zoom In

ARG10112 anti-GAPDH antibody [6C5] WB image

Western blot: 1) U87-MG 2) HepG2 3) rat brain 4) rat heart 5) rat ovary stained with ARG10112 anti-GAPDH antibody [6C5] at 1:2000 dilution.
ARG10112 anti-GAPDH antibody [6C5] ICC/IF image

Immunofluorescence: 100% Methanol fixed (RT, 10 min) HeLa cells stained with ARG10112 anti-GAPDH antibody [6C5] (green) at 1:200 dilution.

Secondary antibody: ARG55393 Goat anti-Mouse IgG (H+L) antibody (FITC)
ARG54747 anti-Histone H3 antibody WB image

Western blot: 20 µg of 293T cell lysate stained with ARG54747 anti-Histone H3 antibody at 1:2000 dilution.
ARG10112 anti-GAPDH antibody [6C5] WB image

Western blot: 1) MCF-7 2) DU-145 3) A549 4) H1299 5) HCT116 6) HepG2 7) HUVEC stained with ARG10112 anti-GAPDH antibody [6C5] at 1:1000 dilution.
ARG10112 anti-GAPDH antibody [6C5] WB image

Western blot: 1) U87-MG 2) MCF-7 3) A549 4) DU145 5) SW480 6) rat brain 7) rat stomach 8) rat ovary stained with ARG10112 anti-GAPDH antibody [6C5] at 1:5000 dilution.
ARG10112 anti-GAPDH antibody [6C5] WB image

Western blot: 1) U87-MG 2) HepG2 3) rat brain 4) rat heart 5) rat ovary stained with ARG10112 anti-GAPDH antibody [6C5] at 1:2000 dilution.
ARG10112 anti-GAPDH antibody [6C5] WB image

Western Blot: 1) HeLa, 2) NTERA-2, 3) A-431, 4) HepG2, 5) MCF-7, 6) NIH 3T3, 7) PC-12 and 8) COS-7 whole cell lysates stained with anti-GAPDH antibody [6C5] (ARG10112)
ARG51013 anti-NFkB p65 antibody WB image

Western Blot: extracts from HeLa and 293 cells stained with anti-NFkB p65 antibody ARG51013.
ARG54746 anti-NFkB p105 / p50 antibody WB image

Western blot: 35 μg of Daudi, Jurkat cell line lysate (from left to right) stained with ARG54746 anti-NFkB p105 / p50 antibody at 1:1000 dilution.
ARG54747 anti-Histone H3 antibody WB image

Western blot: 35 μg of HeLa, MCF-7, C6 cell line lysates (from left to right) stained with ARG54747 anti-Histone H3 antibody at 1:2000 dilution.
ARG54746 anti-NFkB p105 / p50 antibody FACS image

Flow Cytometry: HeLa cells stained with ARG54746 anti-NFkB p105 / p50 antibody (green) at 1:25 dilution or isotype control antibody (blue), followed by incubation with Alexa Fluor® 488 labelled secondary antibody.
ARG54746 anti-NFkB p105 / p50 antibody IHC-P image

Immunohistochemistry: Paraffin-embedded Human spleen section stained with ARG54746 anti-NFkB p105 / p50 antibody at 1:25 dilution.
ARG10112 anti-GAPDH antibody [6C5] IP image

Immunoprecipitation and western blot: 1) GAPDH (1 μg). 2) GAPDH IP from rat heart tissue extract. 3) Only GAPDH preincubated with Protein A Sepharose. 4) Only Protein A Sepharose stained with ARG10112 GAPDH antibody [6C5].

Mixture of protein A-Sepharose with ARG10112 anti-GAPDH and tissue extract was incubated for 30 min at room temperature and precipitated by centrifugation. Pellet was washed with PBS, suspended in reducing electrophoresis sample buffer and heated for 5 minutes at 100ºC. After centrifugation supernatant was loaded on gel and proteins were separated by SDS electrophoresis.
ARG54746 anti-NFkB p105 / p50 antibody WB image

Western blot: 35 µg of Daudi cell lysate stained with ARG54746 anti-NFkB p105 / p50 antibody at 1:1000 dilution.
ARG51013 anti-NFkB p65 antibody IHC-P image

Immunohistochemistry: paraffin-embedded human breast carcinoma tissue stained with anti-NFkB p65 antibody ARG51013 (left) or the same antibody preincubated with blocking peptide (right).
ARG54746 anti-NFkB p105 / p50 antibody IHC image

Immunohistochemistry: paraffin-embedded Human spleen section stained with ARG54746 anti-NFkB p105 / p50 antibody at 1:25 dilution.
ARG54746 anti-NFkB p105 / p50 antibody IHC image

Immunohistochemistry: paraffin-embedded Human tonsil section stained with ARG54746 anti-NFkB p105 / p50 antibody at 1:25 dilution.
ARG54746 anti-NFkB p105 / p50 antibody FACS image

Flow Cytometry: HeLa cells stained with ARG54746 anti-NFkB p105 / p50 antibody (right histogram) at 1:25 dilution or isotype control antibody (left histogram), followed by incubation with Alexa Fluor® 488 labelled secondary antibody.
ARG10112 anti-GAPDH antibody [6C5] WB image

Western blot: 1) U87-MG 2) MCF-7 3) A549 4) DU145 5) SW480 6) rat brain 7) rat stomach 8) rat ovary stained with ARG10112 anti-GAPDH antibody [6C5] at 1:5000 dilution.
ARG51013 anti-NFkB p65 antibody IHC-Fr image

Immunofluorescence: Rat (L1–5) spinal cord stained with ARG51013 anti-NFkB p65 antibody and ARG54348 anti-MyD88 antibody.

From Peng Liu et al. Mediators of inflammation (2018), doi: .org/10.1155/2018/6016272, Fig. 5.
ARG10112 anti-GAPDH antibody [6C5] WB image

Western blot: Mouse samples stained with ARG10112 anti-GAPDH antibody [6C5] at 1:1000 dilution.

From Yun-Yun Li et al. Int J Biol Sci (2022), doi: 10.7150/ijbs.68224, Fig. 5. C.
ARG10112 anti-GAPDH antibody [6C5] WB image

Western blot: Porcine kidney stained with ARG10112 anti-GAPDH antibody [6C5].

From Jianni Huang et al. Front Cell Dev Biol (2022), doi: 10.3389/fcell.2022.899869, Fig. 2. E.
ARG10112 anti-GAPDH antibody [6C5] WB image

Western blot: pAFs, AcoSV40, and AcoSI-1 stained with ARG10112 anti-GAPDH antibody [6C5] at 1:5000 dilution.

From Michele N Dill et al. PLoS One. (2023), doi: 10.3389/fcell.2022.899869, Fig. 2. C.
ARG10112 anti-GAPDH antibody [6C5] WB image

Western blot: 1) MCF-7 2) DU-145 3) A549 4) H1299 5) HCT116 6) HepG2 7) HUVEC stained with ARG10112 anti-GAPDH antibody [6C5] at 1:1000 dilution.

Specific References

The effect of long-term administration of green tea catechins on aging-related cardiac diastolic dysfunction and decline of troponin I

ARG10112: WB / Mouse

Junjun Quan et al.
pre-print, (2024)

PCSK6 exacerbates Alzheimer's disease pathogenesis by promoting MT5-MMP maturation

ARG10112: WB / Human, Mouse

Mingliang Xu et al.
Exp Neurol., (2024)

Biochanin A inhibits cardiac hypertrophy and fibrosis in vivo and in vitro

ARG10112: WB / Mouse, Rat

Zhenyu Feng et al.
Biomed Pharmacother., (2024)

Shionone relieves oxygen-glucose deprivation/reoxygenation induced SH-SY5Y cells injury by inhibiting the p38 MAPK/NF-κB pathway

ARG10112: WB / Human

Xiaoli Zhou et al.
J Cardiothorac Surg, (2024)

7,8-Dihydroxyflavone ameliorates cognitive impairment induced by repeated neonatal sevoflurane exposures in mice through increasing tau O-GlcNAcylation

ARG10112: WB / Mouse

Mingliang Xu et al.
Neurosci Lett., (2024)

黄芪甲苷预处理对大鼠肠缺血再灌注所致肺损伤的影响及其机制

ARG10112: WB / Rat

王蕾 et al.
中国中西医结合外科杂志, (2023)

Discovery of the First Subnanomolar PPARα/δ Dual Agonist for the Treatment of Cholestatic Liver Diseases

ARG10112: WB / Mouse

Zhiqi Feng et al.
J Med Chem., (2023)

Knockdown of Porf-2 restores visual function after optic nerve crush injury

ARG10112: WB / Mouse

Di Chen et al.
Cell Death Dis., (2023)

PPARγ activation suppresses chondrocyte ferroptosis through mitophagy in osteoarthritis

ARG10112: WB / Rat

Xiang Xue et al.
J Orthop Surg Res., (2023)

Resveratrol inhibits Toxoplasma gondii-induced lung injury, inflammatory cascade and evidences of its mechanism of action

ARG10112: WB / Mouse

Yu Nan Lu et al.
Phytomedicine., (2023)

KDF1 Promoted Proliferation, Migration and Invasion of Lung Adenocarcinoma Cells through Activating STAT3 and AKT Pathway

ARG65350: WB /

Yi-Qing Guo et al.
Biomedicines., (2023)

Uric acid mitigates cognitive deficits via transcription factor EB - mediated microglial autophagy in mice models of Alzheimer's disease

ARG10112: WB / Mouse

Qian Xiao et al.
, (2023)

Downregulation of lncRNA NEAT1 alleviates sepsis-induced acute kidney injury

ARG10112: WB / Human

Yuhua Zhou et al.
Cent Eur J Immunol ., (2022)

Thalidomide Alleviates Pulmonary Fibrosis Induced by Silica in Mice by Inhibiting ER Stress and the TLR4-NF-κB Pathway

ARG51013: WB / Mouse

Yaqian Li et al.
International Journal of Molecular Sciences, (2022)

Reproductive toxicity investigation of silica nanoparticles in male pubertal mice

ARG51013: WB / Mouse

Fanli Sun et al.
Environ Sci Pollut Res Int., (2022)

Toxicity Investigation of Nano-SiO2 on Male Reproductive System in Pubertal Mice

ARG51013: WB / Mouse

Fanli Sun et al.
Research Square, (2021)

Cardiac-Specific Gene TNNI3 as a Potential Oncogene for Kidney Cancer and Its Involvement in Wnt Signaling Pathway.

ARG10112: WB / Human

Weian Zhao et al.
Preprint., (2021)

Mesenchymal Stem Cell-Derived Exosomes Ameliorate Alzheimer's Disease Pathology and Improve Cognitive Deficits.

ARG10112: WB / Human

Yi-An Chen et al.
Biomedicines., (2021)

Ac-SDKP Attenuates Activation of Lung Macrophages and Bone Osteoclasts in Rats Exposed to Silica by Inhibition of TLR4 and RANKL Signaling Pathways.

ARG51013: WB / Rat

Fuyu Jin et al.
J Inflamm Res., (2021)

Evaluation of Class IIa Histone Deacetylases Expression and In Vivo Epigenetic Imaging in a Transgenic Mouse Model of Alzheimer's Disease

ARG10112: WB / Human

Yi-An Chen et al.
Int J Mol Sci., (2021)

Circ_0081572 inhibits the progression of periodontitis through regulating the miR-378h/RORA axis.

ARG10112: WB / Human

Jing Wang et al.
Arch Oral Biol., (2021)

Activation of GABAB Receptor Suppresses Diabetic Neuropathic Pain through Toll-Like Receptor 4 Signaling Pathway in the Spinal Dorsal Horn.

ARG51013: WB, IHC-Fr / Rat

Liu Peng et al.
Mediators of inflammation., (2018)

Inflammasome & Pyroptosis

Ferroptosis

Inflammation

ARG30251

NFkB nuclear translocation Antibody Panel

Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Controls and Markers antibody; Gene Regulation antibody; Immune System antibody; Metabolism antibody; Microbiology and Infectious Disease antibody; Neuroscience antibody; Signaling Transduction antibody