ARG30251
NFkB nuclear translocation Antibody Panel
Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Controls and Markers antibody; Gene Regulation antibody; Immune System antibody; Metabolism antibody; Microbiology and Infectious Disease antibody; Neuroscience antibody; Signaling Transduction antibody
Publication20
Component
| Cat No | Component Name | Host clonality | Reactivity | Application | Package |
|---|---|---|---|---|---|
| ARG10112 | anti-GAPDH antibody [6C5] | Mouse mAb | Hu, Ms, Rat, AGMK, Bb, Cat, Chk, Dog, Fsh, Hm, Mk, Pig, Rb, Xenopus laevis, Zfsh | ELISA, ICC/IF, IHC-Fr, WB | 50 μg |
| ARG54747 | anti-Histone H3 antibody | Mouse mAb | Hu | WB | 100 μl |
| ARG54746 | anti-NFkB p105 / p50 antibody | Mouse mAb | Hu | FACS, IHC-P, WB | 100 μl |
| ARG51013 | anti-NFkB p65 antibody | Rabbit pAb | Hu, Ms, Rat | IHC-P, WB | 50 μl |
| ARG65350 | Goat anti-Mouse IgG antibody (HRP) | Goat pAb | Ms | ELISA, IHC-P, WB | 50 μl |
Overview
| Product Description | Nuclear factor kappa B (NFkB) is a protein complex that controls large number of genes responsible for the regulation of apoptosis, inflammation, tumorigenesis and a variety of immune responses. NF-kB is sensitive and responsive to stimuli such as cytokines, stress, UV, low oxygen, bacterial or viral infections. Upon activation, NFkB is translocated into nucleus and exert its function on the transcriptional regulation of NFkB-responsive genes. Histone H3 is a marker for nuclear fraction while GAPDH is a marker for cytosolic fraction. Baeuerle et al. 1991. Biochem Biophys Acta 1072: 63-80 Lenardo and Baltimore. 1989. Cell 58: 227 |
|---|---|
| Target Name | NFkB nuclear translocation |
| Alternate Names | NFkB nuclear translocation antibody; GAPDH antibody; NFkB p65 antibody; NFkB p105 / p50 antibody; Histone H3 antibody |
Properties
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
|---|---|
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Gene Full Name | Antibody Panel for NFkB nuclear translocation |
|---|---|
| Research Area | Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Controls and Markers antibody; Gene Regulation antibody; Immune System antibody; Metabolism antibody; Microbiology and Infectious Disease antibody; Neuroscience antibody; Signaling Transduction antibody |
Images (20) Click the Picture to Zoom In
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ARG10112 anti-GAPDH antibody [6C5] WB image
Western blot: 1) U87-MG 2) HepG2 3) rat brain 4) rat heart 5) rat ovary stained with ARG10112 anti-GAPDH antibody [6C5] at 1:2000 dilution.
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ARG10112 anti-GAPDH antibody [6C5] ICC/IF image
Immunofluorescence: 100% Methanol fixed (RT, 10 min) HeLa cells stained with ARG10112 anti-GAPDH antibody [6C5] (green) at 1:200 dilution.
Secondary antibody: ARG55393 Goat anti-Mouse IgG (H+L) antibody (FITC)
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ARG54747 anti-Histone H3 antibody WB image
Western blot: 20 µg of 293T cell lysate stained with ARG54747 anti-Histone H3 antibody at 1:2000 dilution.
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ARG10112 anti-GAPDH antibody [6C5] WB image
Western blot: 1) MCF-7 2) DU-145 3) A549 4) H1299 5) HCT116 6) HepG2 7) HUVEC stained with ARG10112 anti-GAPDH antibody [6C5] at 1:1000 dilution.
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ARG10112 anti-GAPDH antibody [6C5] WB image
Western blot: 1) U87-MG 2) MCF-7 3) A549 4) DU145 5) SW480 6) rat brain 7) rat stomach 8) rat ovary stained with ARG10112 anti-GAPDH antibody [6C5] at 1:5000 dilution.
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ARG10112 anti-GAPDH antibody [6C5] WB image
Western blot: 1) U87-MG 2) HepG2 3) rat brain 4) rat heart 5) rat ovary stained with ARG10112 anti-GAPDH antibody [6C5] at 1:2000 dilution.
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ARG10112 anti-GAPDH antibody [6C5] WB image
Western Blot: 1) HeLa, 2) NTERA-2, 3) A-431, 4) HepG2, 5) MCF-7, 6) NIH 3T3, 7) PC-12 and 8) COS-7 whole cell lysates stained with anti-GAPDH antibody [6C5] (ARG10112)
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ARG51013 anti-NFkB p65 antibody WB image
Western Blot: extracts from HeLa and 293 cells stained with anti-NFkB p65 antibody ARG51013.
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ARG54746 anti-NFkB p105 / p50 antibody WB image
Western blot: 35 μg of Daudi, Jurkat cell line lysate (from left to right) stained with ARG54746 anti-NFkB p105 / p50 antibody at 1:1000 dilution.
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ARG54747 anti-Histone H3 antibody WB image
Western blot: 35 μg of HeLa, MCF-7, C6 cell line lysates (from left to right) stained with ARG54747 anti-Histone H3 antibody at 1:2000 dilution.
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ARG54746 anti-NFkB p105 / p50 antibody FACS image
Flow Cytometry: HeLa cells stained with ARG54746 anti-NFkB p105 / p50 antibody (green) at 1:25 dilution or isotype control antibody (blue), followed by incubation with Alexa Fluor® 488 labelled secondary antibody.
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ARG54746 anti-NFkB p105 / p50 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human spleen section stained with ARG54746 anti-NFkB p105 / p50 antibody at 1:25 dilution.
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ARG10112 anti-GAPDH antibody [6C5] IP image
Immunoprecipitation and western blot: 1) GAPDH (1 μg). 2) GAPDH IP from rat heart tissue extract. 3) Only GAPDH preincubated with Protein A Sepharose. 4) Only Protein A Sepharose stained with ARG10112 GAPDH antibody [6C5].
Mixture of protein A-Sepharose with ARG10112 anti-GAPDH and tissue extract was incubated for 30 min at room temperature and precipitated by centrifugation. Pellet was washed with PBS, suspended in reducing electrophoresis sample buffer and heated for 5 minutes at 100ºC. After centrifugation supernatant was loaded on gel and proteins were separated by SDS electrophoresis. -
ARG54746 anti-NFkB p105 / p50 antibody WB image
Western blot: 35 µg of Daudi cell lysate stained with ARG54746 anti-NFkB p105 / p50 antibody at 1:1000 dilution.
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ARG51013 anti-NFkB p65 antibody IHC-P image
Immunohistochemistry: paraffin-embedded human breast carcinoma tissue stained with anti-NFkB p65 antibody ARG51013 (left) or the same antibody preincubated with blocking peptide (right).
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ARG54746 anti-NFkB p105 / p50 antibody IHC image
Immunohistochemistry: paraffin-embedded Human spleen section stained with ARG54746 anti-NFkB p105 / p50 antibody at 1:25 dilution.
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ARG54746 anti-NFkB p105 / p50 antibody IHC image
Immunohistochemistry: paraffin-embedded Human tonsil section stained with ARG54746 anti-NFkB p105 / p50 antibody at 1:25 dilution.
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ARG54746 anti-NFkB p105 / p50 antibody FACS image
Flow Cytometry: HeLa cells stained with ARG54746 anti-NFkB p105 / p50 antibody (right histogram) at 1:25 dilution or isotype control antibody (left histogram), followed by incubation with Alexa Fluor® 488 labelled secondary antibody.
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ARG10112 anti-GAPDH antibody [6C5] WB image
Western blot: 1) U87-MG 2) MCF-7 3) A549 4) DU145 5) SW480 6) rat brain 7) rat stomach 8) rat ovary stained with ARG10112 anti-GAPDH antibody [6C5] at 1:5000 dilution.
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ARG10112 anti-GAPDH antibody [6C5] WB image
Western blot: 1) MCF-7 2) DU-145 3) A549 4) H1299 5) HCT116 6) HepG2 7) HUVEC stained with ARG10112 anti-GAPDH antibody [6C5] at 1:1000 dilution.
Specific References
PCSK6 exacerbates Alzheimer's disease pathogenesis by promoting MT5-MMP maturation
ARG10112: WB / Human, Mouse
Biochanin A inhibits cardiac hypertrophy and fibrosis in vivo and in vitro
ARG10112: WB / Mouse, Rat
7,8-Dihydroxyflavone ameliorates cognitive impairment induced by repeated neonatal sevoflurane exposures in mice through increasing tau O-GlcNAcylation
ARG10112: WB / Mouse
The effect of long-term administration of green tea catechins on aging-related cardiac diastolic dysfunction and decline of troponin I
ARG10112: WB / Mouse
Resveratrol inhibits Toxoplasma gondii-induced lung injury, inflammatory cascade and evidences of its mechanism of action
ARG10112: WB / Mouse
KDF1 Promoted Proliferation, Migration and Invasion of Lung Adenocarcinoma Cells through Activating STAT3 and AKT Pathway
ARG65350: WB /
Uric acid mitigates cognitive deficits via transcription factor EB - mediated microglial autophagy in mice models of Alzheimer's disease
ARG10112: WB / Mouse
PPARγ activation suppresses chondrocyte ferroptosis through mitophagy in osteoarthritis
ARG10112: WB / Rat
黄芪甲苷预处理对大鼠肠缺血再灌注所致肺损伤的影响及其机制
ARG10112: WB / Rat
Discovery of the First Subnanomolar PPARα/δ Dual Agonist for the Treatment of Cholestatic Liver Diseases
ARG10112: WB / Mouse
Knockdown of Porf-2 restores visual function after optic nerve crush injury
ARG10112: WB / Mouse
Downregulation of lncRNA NEAT1 alleviates sepsis-induced acute kidney injury
ARG10112: WB / Human
Thalidomide Alleviates Pulmonary Fibrosis Induced by Silica in Mice by Inhibiting ER Stress and the TLR4-NF-κB Pathway
ARG51013: WB / Mouse
Reproductive toxicity investigation of silica nanoparticles in male pubertal mice
ARG51013: WB / Mouse
Mesenchymal Stem Cell-Derived Exosomes Ameliorate Alzheimer's Disease Pathology and Improve Cognitive Deficits.
ARG10112: WB / Human
Ac-SDKP Attenuates Activation of Lung Macrophages and Bone Osteoclasts in Rats Exposed to Silica by Inhibition of TLR4 and RANKL Signaling Pathways.
ARG51013: WB / Rat
Evaluation of Class IIa Histone Deacetylases Expression and In Vivo Epigenetic Imaging in a Transgenic Mouse Model of Alzheimer's Disease
ARG10112: WB / Human
Circ_0081572 inhibits the progression of periodontitis through regulating the miR-378h/RORA axis.
ARG10112: WB / Human
Toxicity Investigation of Nano-SiO2 on Male Reproductive System in Pubertal Mice
ARG51013: WB / Mouse
Cardiac-Specific Gene TNNI3 as a Potential Oncogene for Kidney Cancer and Its Involvement in Wnt Signaling Pathway.
ARG10112: WB / Human
