ARG62347

anti-beta Tubulin antibody [BT7R]

anti-beta Tubulin antibody [BT7R] for Dot blot,ELISA,ICC/IF,Western blot and Human,Mouse,Rat,Chicken,Monkey,Rabbit

Controls and Markers antibody; Signaling Transduction antibody
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publication_link Publication8

Overview

Product Description Mouse Monoclonal antibody [BT7R] recognizes beta Tubulin
Tested Reactivity Hu, Ms, Rat, Chk, Mk, Rb
Tested Application Dot, ELISA, ICC/IF, WB
Specificity Recognizes native and denatured forms of beta Tubulin (about 50 kDa).
Host Mouse
Clonality Monoclonal
Clone BT7R
Isotype IgG2a
Target Name beta Tubulin
Antigen Species Human
Immunogen KLH-conjugated synthetic peptide around the N-terminal region of Human beta Tubulin.
Conjugation Un-conjugated
Alternate Names Tubulin beta-1 chain

Application Instructions

Application Suggestion
Tested Application Dilution
DotAssay-dependent
ELISAAssay-dependent
ICC/IF1:500 - 1:2000
WB1:2000 - 1:10000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control Mouse brain tissue lysates

Properties

Form Liquid
Purification Note Protein A affinity chromatography from Mouse ascites fluid.
Buffer 10mM PBS (pH 7.2) and 0.05% Sodium azide
Preservative 0.05% Sodium azide
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 396427 Chicken TUBB1

GeneID: 545486 Mouse TUBB1

GeneID: 81027 Human TUBB1

Gene Symbol TUBB1
Gene Full Name tubulin, beta 1 class VI
Background Beta tubulins are one of two core protein families (alpha and beta tubulins) that heterodimerize and assemble to form microtubules. This protein is specifically expressed in platelets and megakaryocytes and may be involved in proplatelet production and platelet release. A mutations in this gene is associated with autosomal dominant macrothrombocytopenia. Two pseudogenes of this gene are found on chromosome Y. [provided by RefSeq, Jul 2010]
Function Tubulins is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain. [UniProt]
Cellular Localization Cytoplasm › cytoskeleton
Highlight Related Antibody Duos and Panels:
ARG30330 Pyroptosis Antibody Panel
Related products:
beta Tubulin antibodies; beta Tubulin Duos / Panels; Anti-Mouse IgG secondary antibodies;
Research Area Controls and Markers antibody; Signaling Transduction antibody
Calculated MW 50 kDa
PTM Some glutamate residues at the C-terminus are polyglutamylated, resulting in polyglutamate chains on the gamma-carboxyl group (PubMed:26875866). Polyglutamylation plays a key role in microtubule severing by spastin (SPAST). SPAST preferentially recognizes and acts on microtubules decorated with short polyglutamate tails: severing activity by SPAST increases as the number of glutamates per tubulin rises from one to eight, but decreases beyond this glutamylation threshold (PubMed:26875866).
Some glutamate residues at the C-terminus are monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella). Both polyglutamylation and monoglycylation can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of monoglycylation is still unclear (Probable).
Phosphorylated on Ser-172 by CDK1 during the cell cycle, from metaphase to telophase, but not in interphase. This phosphorylation inhibits tubulin incorporation into microtubules.

Images (3) Click the Picture to Zoom In

  • ARG62347 anti-beta Tubulin antibody [BT7R] WB image

    Western blot: 20 μg of PC3, MCF7, MDA-MD-231, K562, M. brain, R. brain, MCF7, HT29, 3T3 and Raw264.7 cell lysates stained with ARG62347 anti-beta Tubulin antibody [BT7R] at 1:3000 dilution.

  • ARG62347 anti-beta Tubulin antibody [BT7R] WB image

    Western blot: 20 µg of 293T, Mouse brain and Rat brain lysates stained with ARG62347 anti-beta Tubulin antibody [BT7R] at 1:10000 dilution.

  • ARG62347 anti-beta Tubulin antibody [BT7R] WB image

    Western blot: U87-MG and SW480 cell lysates stained with ARG62347 anti-beta Tubulin antibody [BT7R] at 1:1000 dilution.

Customer's Feedback

nuts_pic      Excellent

Review for anti-beta Tubulin antibody [BT7R]

Application:WB

Sample:293T, Mouse brain and Rat brain

Sample Loading Amount:20 µg

Primary Antibody Dilution Factor:1:10000

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

nuts_pic      Excellent

Review for anti-beta Tubulin antibody [BT7R]

Application:WB

Sample:PC3, MCF7, MDA-MD-231, K562, M. brain, R. Brain, MCF7, HT29, 3T3, and Raw264.7

Sample Loading Amount:20 µg

Primary Antibody Dilution Factor:1:3000

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

nuts_pic      Excellent

Review for anti-beta Tubulin antibody [BT7R]

Application:WB

Sample:K562, Mouse brain and Rat brain

Sample Loading Amount:20 µg

Primary Antibody Dilution Factor:1:4000

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

nuts_pic      Excellent

Review for anti-beta Tubulin antibody [BT7R]

Application:WB

Sample:HEK293T, HepG2 and Jurkat cells

Sample Loading Amount:20 µg

Primary Antibody Dilution Factor:1:3000

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

nuts_pic      Good

Review for anti-beta Tubulin antibody [BT7R]

Application:WB

Sample:293T and HeLa cells

Sample Loading Amount:20 µg

Primary Antibody Dilution Factor:1:2000

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

Specific References

KDM3A Ablation Activates Endogenous Retrovirus Expression to Stimulate Antitumor Immunity in Gastric Cancer

WB / Human

Jiabin Zheng et al.
Adv Sci (Weinh).,  (2024)

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Overexpression of EphB2 in the basolateral amygdala is crucial for inducing visceral pain sensitization in rats subjected to water avoidance stress

WB / Rat

Guang-Bing Duan et al.
CNS Neurosci Ther.,  (2024)

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Endophilin A2 controls touch and mechanical allodynia via kinesin-mediated Piezo2 trafficking

WB / Mouse

Man-Xiu Xie et al.
Mil Med Res.,  (2024)

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STING activation in cardiomyocytes drives hypertrophy-associated heart failure via NF-κB-mediated inflammatory response

WB / Human, Mouse

Lintao Wang et al.
Biochim Biophys Acta Mol Basis Dis.,  (2023)

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Upregulation of Netrin-1 in the hippocampus mediates the formation of visceral hypersensitivity induced by maternal separation

WB / Mouse

Junwen Wang et al.
Front Mol Neurosci.,  (2022)

publication_link

 

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Protection against Doxorubicin-Induced Cardiotoxicity through Modulating iNOS/ARG 2 Balance by Electroacupuncture at PC6.

WB / Mouse

Jingya Wang et al.
Oxid Med Cell Longev.,  (2021)

publication_link

 

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ATF4 selectively regulates heat nociception and contributes to kinesin-mediated TRPM3 trafficking.

WB / Mouse

Man-Xiu Xie et al.
Nat Commun.,  (2021)

publication_link

 

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Foxo1 selectively regulates static mechanical pain by interacting with Nav1.7.

WB / Rat

Xiao-Long Zhang et al.
Pain.,  (2021)

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