ARG30258

Loading Controls for Whole Cell Lysate Antibody Panel

Cancer antibody; Cell Biology and Cellular Response antibody; Controls and Markers antibody; Immune System antibody; Metabolism antibody; Signaling Transduction antibody
publication_link Publication61

Component

Cat No Component Name Host clonality Reactivity Application Package
ARG65714 anti-Vinculin antibody Rabbit pAb Hu, Ms, Rat WB 25 μl
ARG65693 anti-alpha Tubulin antibody Rabbit pAb Hu, Ms, Rat FACS, ICC/IF, IHC-P, WB 25 μl
ARG65683 anti-beta Actin antibody Rabbit pAb Hu, Ms, Rat, Rb, Sheep IHC-P, WB 25 μg
ARG65680 anti-GAPDH antibody Rabbit pAb Hu, Ms, Rat ICC/IF, IHC-P, WB 25 μl
ARG65351 Goat anti-Rabbit IgG antibody (HRP) Goat pAb Rb ELISA, IHC-P, WB 50 μl

Overview

Target Name Loading Controls for Whole Cell Lysate
Alternate Names Loading Controls for Whole Cell Lysate antibody; GAPDH antibody; beta Actin antibody; alpha Tubulin antibody; Vinculin antibody

Properties

Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Gene Full Name Antibody Panel for Loading Controls for Whole Cell Lysate
Highlight Related poster download:
Organelle Markers & Loading Control
Research Area Cancer antibody; Cell Biology and Cellular Response antibody; Controls and Markers antibody; Immune System antibody; Metabolism antibody; Signaling Transduction antibody

Images (22) Click the Picture to Zoom In

  • ARG65680 anti-GAPDH antibody WB image

    Western blot: 20 µg of 293T cell lysate stained with ARG65680 anti-GAPDH antibody at 1:10000 dilution.

  • ARG65680 anti-GAPDH antibody WB image

    Western blot: 20 µg of 22RV1 and DU145 cell lysates stained with ARG66302 anti-Vimentin antibody [SQab1859] at 1:2000 dilution and ARG65680 anti-GAPDH antibody at 1:10000 dilution.

  • ARG65680 anti-GAPDH antibody WB image

    Western blot: 20 µg of COLO205 and SW620 cell lysates stained with ARG66302 anti-Vimentin antibody [SQab1859] at 1:2000 dilution and ARG65680 anti-GAPDH antibody at 1:10000 dilution.

  • ARG65683 anti-beta Actin antibody WB image

    Western blot: 20 µg of HeLa, Mouse brain and Rat brain lysates stained with ARG65683 anti-beta Actin antibody at 1:10000 dilution.

  • ARG65683 anti-beta Actin antibody WB image

    Western blot: 30 µg of 293T lysate stained with ARG65683 anti-beta Actin antibody at 1:3000 dilution.

  • ARG65683 anti-beta Actin antibody WB image

    Western blot: 30 µg of 1) Rat brain, and 2) Mouse liver lysate stained with ARG65683 anti-beta Actin antibody at 1:3000 dilution.

  • ARG65693 anti-alpha Tubulin antibody ICC/IF image

    Immunofluorescence: 100% Methanol fixed (RT, 10 min) HeLa cells stained with ARG65693 anti-alpha Tubulin antibody at 1:200 dilution. Left: primary antibody (orange). Middle: DAPI (blue). Right: Merge.

    Secondary antibody: ARG21917 Goat anti-Rabbit IgG antibody (TRITC)

  • ARG65693 anti-alpha Tubulin antibody WB image

    Western blot: 20 µg of 293T, Mouse brain and Rat brain lysates stained with ARG65693 anti-alpha Tubulin antibody at 1:10000 dilution.

  • ARG65714 anti-Vinculin antibody WB image

    Western blot: 30 µg of HeLa treated with TNFa at 0, 10, 20, and 30 min. The blots stained with ARG51850 anti-P38 MAPK phospho (Thr180/Tyr182) antibody, ARG55258 anti-p38 MAPK antibody, and ARG65714 anti-Vinculin antibody.

  • ARG65680 anti-GAPDH antibody WB image

    Western blot: 30 µg of HeLa cell lysate stained with ARG65680 anti-GAPDH antibody at 1:500 dilution.

  • ARG65680 anti-GAPDH antibody ICC/IF image

    Immunofluorescence: MCF-7 stained with ARG65680 anti-GAPDH antibody. Blue: DAPI for nuclear staining.

  • ARG65683 anti-beta Actin antibody WB image

    Western blot: 1) HepG2, 2) Rat liver, 3) Mouse kidney, 4) Rabbit testis, 5) Sheep lung, and 6) 293T lysates stained with ARG65683 anti-beta Actin antibody at 1:5000 dilution.

  • ARG65714 anti-Vinculin antibody WB image

    Western blot: extracts of various cell lines stained with ARG65714 anti-Vinculin antibody.

  • ARG65680 anti-GAPDH antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Mouse heart stained with ARG65680 anti-GAPDH antibody at 1:100 dilution.

  • ARG65680 anti-GAPDH antibody IHC-P image

    Immunohistochemistry: paraffin-embedded Rat pancreas stained with ARG65680 anti-GAPDH antibody at 1:100 dilution (400x lens).

  • ARG65683 anti-beta Actin antibody IHC-P image

    Immunohistochemistry: Human ovary tissue stained with ARG65683 anti-beta Actin antibody at 1:200 dilution.

  • ARG65693 anti-alpha Tubulin antibody WB image

    Western blot: 30 µg of HeLa cell lysate stained with ARG65693 anti-alpha Tubulin antibody at 1:5000 dilution.

  • ARG65714 anti-Vinculin antibody WB image

    Western blot: 30 µg of Mouse heart, and HeLa cell lysate stained with ARG65714 anti-Vinculin antibody at 1:500 dilution.

  • ARG65680 anti-GAPDH antibody WB image

    Western blot: 20 µg of 293T and HeLa cell lysate stained with ARG65680 anti-GAPDH antibody at 1:3000 dilution.

  • ARG65693 anti-alpha Tubulin antibody WB image

    Western blot: 30 µg of 1) Mouse brain, and 2) Rat brain lysates stained with ARG65693 anti-alpha Tubulin antibody at 1:500 dilution.

  • ARG65680 anti-GAPDH antibody WB image

    Western blot: 20 µg of COLO205, HCT116, HT29, SW480 and SW620 cell lysates stained with ARG10756 anti-HMGB1 antibody [1F3] (1:2000) and ARG65680 anti-GAPDH antibody (1:10000).

  • ARG65680 anti-GAPDH antibody WB image

    Western blot: 30 µg of 1) MDA-MB-231, and 2) MCF-7 lysates stained with ARG65680 anti-GAPDH antibody at 1:5000 dilution.

Specific References

Discovery and mechanism of anti-hypertensive effect of a novel tripeptide (SYP) from medicinal fungus Ganoderma lingzhi

ARG65683: WB / Human

Qiang Wu et al.
Pre-Print,  (2024)

publication_link

 

hr_line

A novel function and mechanism of ischemia-induced retinal astrocyte-derived exosomes for RGC apoptosis of ischemic retinopathy

ARG65693: WB / Mouse

Xiaoyuan Ye et al.
Molecular Therapy,  (2024)

publication_link

 

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Engineering supramolecular dynamics of self-assembly and turnover of oncogenic microRNAs to drive their synergistic destruction in tumor models

ARG65680: WB / Human

Daria Chiglintseva et al.
Biomaterials.,  (2024)

publication_link

 

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Pharmacokinetic enhancement of oncolytic virus M1 by inhibiting JAK‒STAT pathway

ARG65693: WB / Mouse

Jingyi Tan et al.
Acta Pharmaceutica Sinica B,  (2024)

publication_link

 

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Detection of micro-plasma-induced exosomes secretion in a fibroblast-melanoma co-culture model

ARG65693: WB / Mouse

Han Lee et al.
Analytica Chimica Acta,  (2023)

publication_link

 

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Sleep deprivation during pregnancy leads to poor fetal outcomes in Sprague-Dawley rats

ARG65693: WB / Rat

Jinzhi Li et al.
J Reprod Immunol.,  (2023)

publication_link

 

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IDH1 mutation impairs antiviral response and potentiates oncolytic virotherapy in glioma

ARG65693: WB / 

Xueqin Chen et al.
Nat Commun.,  (2023)

publication_link

 

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RCC2 promotes prostate cancer cell proliferation and migration through Hh/GLI1 signaling pathway and cancer stem-like cells

ARG65680: WB / Human

Shenghan Wang et al.
Biol Direct.,  (2023)

publication_link

 

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Targeting VCP potentiates immune checkpoint therapy for colorectal cancer

ARG65693: WB / Mouse

Fang Wang et al.
Cell Rep.,  (2023)

publication_link

 

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Fe3O4 Nanozymes Improve Neuroblast Differentiation and Blood-Brain Barrier Integrity of the Hippocampal Dentate Gyrus in D-Galactose-Induced Aged Mice

ARG65683: WB / Mouse

Zihao Xia et al.
International Journal o f Molecular Sciences,  (2022)

publication_link

 

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An auto-antibody identified from phenotypic directed screening platform shows host immunity against EV-A71 infection

ARG65683: WB / Human

Yu-Wei Cheng et al.
J Biomed Sci.,  (2022)

publication_link

 

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Directly targeting ASC by lonidamine alleviates inflammasome-driven diseases

ARG65693: WB / Mouse

Chen Chen et al.
J Neuroinflammation,  (2022)

publication_link

 

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SOX2 regulates paclitaxel resistance of A549 non‑small cell lung cancer cells via promoting transcription of ClC‑3

ARG65680: WB / Human

Youwei Huang et al.
Oncol Rep,  (2022)

publication_link

 

hr_line

MicroRNA-145 Delay Plaque Erosion by Down-Regulating TGFβr Ii and Suppressing Autophagy of Vascular Smooth Muscle Cells.

ARG65351: WB / Rabbit

Yunjuan Jiao et al.
Preprint.,  (2021)

publication_link

 

hr_line

Baicalin promotes chondrocyte viability and the synthesis of extracellular matrix through TGF-β/Smad3 pathway in chondrocytes

ARG65351: WB / Rabbit

Pengzhen Wang et al.
Am J Transl Res.,  (2021)

publication_link

 

hr_line

Circ_0081572 inhibits the progression of periodontitis through regulating the miR-378h/RORA axis.

ARG65351: WB / Rabbit

Jing Wang et al.
Arch Oral Biol.,  (2021)

publication_link

 

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Design, Synthesis, and Activity Evaluation of Novel Acyclic Nucleosides as Potential Anticancer Agents In Vitro and In Vivo.

ARG65351: WB / Rabbit

Er-Jun Hao et al.
J Med Chem.,  (2021)

publication_link

 

hr_line

Tight junction protein 1 promotes vasculature remodeling via regulating USP2/TWIST1 in bladder cancer

ARG65680: WB / Human

Xue-Qi Liu et al.
Oncogene.,  (2021)

publication_link

 

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Intermittent pressure imitating rolling manipulation ameliorates injury in skeletal muscle cells through oxidative stress and lipid metabolism signalling pathways.

ARG65351: WB / Rabbit

Li-Juan Zhao et al.
Gene.,  (2021)

publication_link

 

hr_line

Inactivation of the tumor suppressor p53 by long noncoding RNA RMRP.

ARG65351: WB / Rabbit

Yajie Chen et al.
Proc Natl Acad Sci.,  (2021)

publication_link

 

hr_line

Predicting the effects of radiotherapy based on diffusion kurtosis imaging in a xenograft mouse model of esophageal carcinoma

ARG65351: WB / Rabbit

An-Du Zhang et al.
Exp Ther Med.,  (2021)

publication_link

 

hr_line

Down-regulation of calreticulin promotes apoptosis in hepatic stellate cells.

ARG65351, ARG65683: WB / Human

Linyu Dai et al.
第三军医大学学报,  (2021)

publication_link

 

hr_line

The Essential Role of Stathmin in Myoblast C2C12 for Vertical Vibration-Induced Myotube Formation

ARG65683: WB / Rat

Yi-Hsiung Lin et al.
Biomedicines .,  (2021)

publication_link

 

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Identi cation of Potential Novel Pleiotropic Susceptibility Variants Common To Lumbar Spine Bone Mineral Density And Birth Weight.

ARG65351: IHC-P / Rabbit

Yu-Qian Song et al.
Preprint.,  (2021)

publication_link

 

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Decidual NR2F2-Expressing CD4 + T Cells Promote TH2 Transcriptional Program During Early Pregnancy.

ARG65351: WB / Rabbit

Yikong Lin et al.
Front Immunol.,  (2021)

publication_link

 

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4-phenylbutyric acid alleviates bleomycin-induced pulmonary fibrosis in mouse via inhibition of endoplasmic reticulum stress.

ARG65683: WB / Mouse

Yun Yang  et al.
Preprint.,  (2020)

publication_link

 

hr_line

SMARCB1 Promotes Ubiquitination and Degradation of NR4A3 via Direct Interaction Driven by ROS in Vascular Endothelial Cell Injury

ARG65351: WB / Rabbit

Bingzheng Lu et al.
Oxid Med Cell Longev.,  (2020)

publication_link

 

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High expression of FUSE binding protein 1 in breast cancer stimulates cell proliferation and diminishes drug sensitivity.

ARG65351: WB / Rabbit

Liu Wei et al.
Int J Oncol.,  (2020)

publication_link

 

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Complete zwitterionic double network hydrogels with great toughness and resistance against foreign body reaction and thrombus.

ARG65351: ELISA / Rabbit

Huang Kang-Ting et al.
J Mater Chem B.,  (2020)

publication_link

 

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Baicalin promotes extracellular matrix synthesis in chondrocytes via the activation of hypoxia-inducible factor-1α

ARG65351: WB / Rabbit

Pengzhen Wang et al.
Exp Ther Med.,  (2020)

publication_link

 

hr_line

Effect of ERK5 on the expression of iNOS and MCP-1 in M1 type macrophages.

ARG65351: WB / Rabbit

Guan Xinzhu et al.
Journal of Jinan University.,  (2020)

publication_link

 

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Melatonin alleviates progression of uterine endometrial cancer by suppressing estrogen/ubiquitin C/SDHB-mediated succinate accumulation.

ARG65351: WB / Rabbit

Gu Chunjie et al.
Cancer Lett.,  (2020)

publication_link

 

hr_line

CLC-3 and SOX2 regulate the cell cycle in DU145 cells

ARG65351: WB / Rabbit

Jiahong Chen et al.
Oncol Lett.,  (2020)

publication_link

 

hr_line

BCL7C suppresses ovarian cancer growth by inactivating mutant p53.

ARG65351: WB / Rabbit

Canhua Huang et al.
J Mol Cell Biol.,  (2020)

publication_link

 

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The effect of TLR4 on the growth and local inflammatory microenvironment of HPV-related cervical cancer in vivo.

ARG65351: IHC-P / Rabbit

Jiang Ninghong et al.
Infect Agent Cancer.,  (2020)

publication_link

 

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Codelivery of Anti-PD-1 Antibody and Paclitaxel with Matrix Metalloproteinase and pH Dual-Sensitive Micelles for Enhanced Tumor Chemoimmunotherapy.

ARG65351: WB, IHC-P / Rabbit

Su Zhenwei et al.
Small.,  (2020)

publication_link

 

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miR-765 Impairs Pancreatic β-cell Function by Targeting PDX1 in type 2 Diabetes.

ARG65351: WB / Rabbit

Li Zheng et al.
Preprint.,  (2020)

publication_link

 

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Ubiquitin ligase DTX3 empowers mutant p53 to promote ovarian cancer development

ARG65351: WB / Rabbit

Shanshan Wang et al.
Genes Dis .,  (2020)

publication_link

 

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Icariin increases chondrocyte vitality by promoting hypoxia-inducible factor-1α expression and anaerobic glycolysis.

ARG65351: WB / Rabbit

Wang Pengzhen et al.
Knee.,  (2019)

publication_link

 

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Down-regulation of HTR1A-modulated ACC activation contributes to stress-induced visceral hyperalgesia in rats.

ARG65351: WB / Rabbit

Yi Lisha et al.
Neurogastroenterol Motil.,  (2019)

publication_link

 

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Ubiquitin ligase TRIM71 suppresses ovarian tumorigenesis by degrading mutant p53.

ARG65351: WB / Rabbit

Chen Yajie et al.
Cell Death and Disease.,  (2019)

publication_link

 

hr_line

Icariin suppresses cell cycle transition and cell migration in ovarian cancer cells.

ARG65351: WB / Rabbit

Wang Pengzhen et al.
Oncol Rep.,  (2019)

publication_link

 

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miR-30b-5p acts as a tumor suppressor microRNA in esophageal squamous cell carcinoma.

ARG65680, ARG65351: WB / Human, Rabbit

Xu Jianfeng et al.
J Thorac Dis.,  (2019)

publication_link

 

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A Novel Synthetic Steroid of 2β,3α,5α-Trihydroxy-androst-6-one Alleviates the Loss of Rat Retinal Ganglion Cells Caused by Acute Intraocular Hypertension via Inhibiting the Inflammatory Activation of Microglia.

ARG65693: WB / Mouse

Sun Hong-Jia-Qi et al.
Molecules.,  (2019)

publication_link

 

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Negative Correlation Between Serum Levels of Homocysteine and Apolipoprotein M.

ARG65680: WB / 

Wei J et al.
Current Molecular Medicine.,  (2019)

publication_link

 

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The Different Effects of IFN- β and IFN- γ on the Tumor-Suppressive Activity of Human Amniotic Fluid-Derived Mesenchymal Stem Cells

ARG65683: WB / Human

Jingchun Du et al.
Stem Cells Int.,  (2019)

publication_link

 

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Alterations ofestradiol-induced histone H3 acetylation in the preoptic area and anteroventral periventricular nucleus of middle-aged female rats.

ARG65351: IHC-P / Rabbit

Xu Wen et al.
Biochem Biophys Res Commun.,  (2019)

publication_link

 

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Negative regulation of miR-1275 by H3K27me3 is critical for glial induction of glioblastoma cells.

ARG65693: WB / Human

Mai J et al.
Mol Oncol.,  (2019)

publication_link

 

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Dengue virus nonstructural protein 1 activates platelets via Toll-like receptor 4, leading to thrombocytopenia and hemorrhage.

ARG65683: WB / Human

Chao Chiao-Hsuan et al.
PLoS Pathog.,  (2019)

publication_link

 

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Treatment of diabetic mice with A Combination of Ketogenic Diet and Aerobic Exercise via modulations of PPARs gene programs.

ARG65351: WB / Rabbit

Zhang Qiang et al.
PPAR Research.,  (2018)

publication_link

 

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The antiepileptic drug levetiracetam promotes neuroblast differentiation and expression of superoxide dismutase in the mouse hippocampal dentate gyrus via PI3K/Akt signalling.

ARG65683: WB / Mouse

Yan Bing-Chun et al.
Neurosci Lett.,  (2018)

publication_link

 

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DNA-PK inhibition synergizes with oncolytic virus M1 by inhibiting antiviral response and potentiating DNA damage.

ARG65693: WB / Human

Xiao Xiao et al.
Nat Commun.,  (2018)

publication_link

 

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Short-term urea cycle inhibition in rat liver cells induced by polyethylene glycol

ARG65351: WB / Rabbit

Xu Li et al.
Biomaterials Science.,  (2018)

publication_link

 

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Knockdown of LGALS12 inhibits porcine adipocyte adipogenesis via PKA-Erk1/2 signaling pathway

ARG65351: WB / Rabbit

Wu Wenjing et al.
Acta Biochim Biophys Sin.,  (2018)

publication_link

 

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Molecular insights for the biological interactions between polyethylene glycol and cells.

ARG65351: WB / Rabbit

Xu Li et al.
Biomaterials.,  (2017)

publication_link

 

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A novel method of neural differentiation of PC12 cells by using Opti-MEM as a basic induction medium.

ARG65351: WB / Rat

Hu Rendong et al.
International Journal of Molecular Medicine.,  (2017)

publication_link

 

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Effect of palmitic acid on caspase-12 and proliferation and apoptosis of hepatic stellate cells

ARG65351: IHC-P / Rabbit

李雅楠 et al.
China Journal of Modern Medicine,  (2017)

publication_link

 

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Topiramate Improves Neuroblast Differentiation of Hippocampal Dentate Gyrus in the D-Galactose-Induced Aging Mice via Its Antioxidant Effects.

ARG65683: WB / Mouse

Shen H et al.
Cell Mol Neurobiol.,  (2016)

publication_link

 

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Study on the inhibition of hyperthermic CO₂ pneumoperitoneum on the proliferation and migration of colon cancer cells and its mechanism.

ARG65351: WB / Rabbit

Zhao J et al.
Oncol Rep.,  (2016)

publication_link

 

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1-Methyl-tryptophan attenuates regulatory T cells differentiation due to the inhibition of estrogen-IDO1-MRC2 axis in endometriosis.

ARG65351: WB / Rabbit

Wei C et al.
Cell Death Dis.,  (2016)

publication_link

 

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Voluntary wheel exercise alters the levels of miR-494 and miR-696 in the skeletal muscle of C57BL/6 mice.

ARG65351: WB / Rabbit

Sun Y et al.
Comp Biochem Physiol B Biochem Mol Biol.,  (2016)

publication_link

 

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