ARG65680
anti-GAPDH antibody
anti-GAPDH antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
Cancer antibody; Controls and Markers antibody; Metabolism antibody; Neuroscience antibody; Signaling Transduction antibody; Loading Control antibody; Loading Control antibody for Cytoplasmic Fractions; Organelle Marker antibody for Cytoplasm; Autophagy Study antibody
Publication7
Overview
| Product Description | Rabbit Polyclonal antibody recognizes GAPDH |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | ICC/IF, IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | GAPDH |
| Antigen Species | Human |
| Immunogen | Fusion protein of Human GAPDH. |
| Conjugation | Un-conjugated |
| Alternate Names | G3PD; GAPD; HEL-S-162eP; Peptidyl-cysteine S-nitrosylase GAPDH; HEL S162eP; Glyceraldehyde-3-phosphate dehydrogenase |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 50% Glycerol |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | GAPDH |
| Gene Full Name | glyceraldehyde-3-phosphate dehydrogenase |
| Background | GAPDH protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The encoded protein has additionally been identified to have uracil DNA glycosylase activity in the nucleus. Also, this protein contains a peptide that has antimicrobial activity against E. coli, P. aeruginosa, and C. albicans. Studies of a similar protein in mouse have assigned a variety of additional functions including nitrosylation of nuclear proteins, the regulation of mRNA stability, and acting as a transferrin receptor on the cell surface of macrophage. Many pseudogenes similar to this locus are present in the human genome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2014] |
| Function | GAPDH has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC. Modulates the organization and assembly of the cytoskeleton. Facilitates the CHP1-dependent microtubule and membrane associations through its ability to stimulate the binding of CHP1 to microtubules. Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate. Component of the GAIT (gamma interferon-activated inhibitor of translation) complex which mediates interferon-gamma-induced transcript-selective translation inhibition in inflammation processes. Upon interferon-gamma treatment assembles into the GAIT complex which binds to stem loop-containing GAIT elements in the 3'-UTR of diverse inflammatory mRNAs (such as ceruplasmin) and suppresses their translation. [UniProt] |
| Highlight | Related Antibody Duos and Panels: ARG30258 Loading Controls for Whole Cell Lysate Antibody Panel Related products: GAPDH antibodies; GAPDH Duos / Panels; Anti-Rabbit IgG secondary antibodies; |
| Research Area | Cancer antibody; Controls and Markers antibody; Metabolism antibody; Neuroscience antibody; Signaling Transduction antibody; Loading Control antibody; Loading Control antibody for Cytoplasmic Fractions; Organelle Marker antibody for Cytoplasm; Autophagy Study antibody |
| Calculated MW | 36 kDa |
| PTM | S-nitrosylation of Cys-152 leads to interaction with SIAH1, followed by translocation to the nucleus (By similarity). S-nitrosylation of Cys-247 is induced by interferon-gamma and LDL(ox) implicating the iNOS-S100A8/9 transnitrosylase complex and seems to prevent interaction with phosphorylated RPL13A and to interfere with GAIT complex activity. ISGylated. Sulfhydration at Cys-152 increases catalytic activity. Oxidative stress can promote the formation of high molecular weight disulfide-linked GAPDH aggregates, through a process called nucleocytoplasmic coagulation. Such aggregates can be observed in vivo in the affected tissues of patients with Alzheimer disease or alcoholic liver cirrhosis, or in cell cultures during necrosis. Oxidation at Met-46 may play a pivotal role in the formation of these insoluble structures. This modification has been detected in vitro following treatment with free radical donor (+/-)-(E)-4-ethyl-2-[(E)-hydroxyimino]-5-nitro-3-hexenamide. It has been proposed to destabilize nearby residues, increasing the likelihood of secondary oxidative damages, including oxidation of Tyr-45 and Met-105. This cascade of oxidations may augment GAPDH misfolding, leading to intermolecular disulfide cross-linking and aggregation. |
Images (9) Click the Picture to Zoom In
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ARG65680 anti-GAPDH antibody WB image
Western blot: 20 µg of 293T cell lysate stained with ARG65680 anti-GAPDH antibody at 1:10000 dilution.
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ARG65680 anti-GAPDH antibody WB image
Western blot: 20 µg of 22RV1 and DU145 cell lysates stained with ARG66302 anti-Vimentin antibody [SQab1859] at 1:2000 dilution and ARG65680 anti-GAPDH antibody at 1:10000 dilution.
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ARG65680 anti-GAPDH antibody WB image
Western blot: 20 µg of COLO205 and SW620 cell lysates stained with ARG66302 anti-Vimentin antibody [SQab1859] at 1:2000 dilution and ARG65680 anti-GAPDH antibody at 1:10000 dilution.
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ARG65680 anti-GAPDH antibody ICC/IF image
Immunofluorescence: MCF-7 stained with ARG65680 anti-GAPDH antibody. Blue: DAPI for nuclear staining.
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ARG65680 anti-GAPDH antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Mouse heart stained with ARG65680 anti-GAPDH antibody at 1:100 dilution.
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ARG65680 anti-GAPDH antibody WB image
Western blot: 20 µg of 293T and HeLa cell lysate stained with ARG65680 anti-GAPDH antibody at 1:3000 dilution.
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ARG65680 anti-GAPDH antibody WB image
Western blot: 20 µg of COLO205, HCT116, HT29, SW480 and SW620 cell lysates stained with ARG10756 anti-HMGB1 antibody [1F3] (1:2000) and ARG65680 anti-GAPDH antibody (1:10000).
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ARG65680 anti-GAPDH antibody WB image
Western blot: 30 µg of 1) MDA-MB-231, and 2) MCF-7 lysates stained with ARG65680 anti-GAPDH antibody at 1:5000 dilution.
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ARG65680 anti-GAPDH antibody WB image
Western blot: 30 µg of HeLa cell lysate stained with ARG65680 anti-GAPDH antibody at 1:500 dilution.
Customer's Feedback
Excellent
Review for anti-GAPDH antibody
Application：WB
Sample：COLO205 and SW620
Sample Loading Amount：20 µg
Primary Antibody Dilution Factor：1:10000
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
Excellent
Review for anti-GAPDH antibody
Application：WB
Sample：22RV1 and DU145
Sample Loading Amount：20 µg
Primary Antibody Dilution Factor：1:10000
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
Excellent
Review for anti-GAPDH antibody
Application：WB
Sample：293T, Mouse brain and Rat brain
Sample Loading Amount：20 µg
Primary Antibody Dilution Factor：1:10000
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
Excellent
Review for anti-GAPDH antibody
Application：WB
Sample：A: MDA-MB-231, B: MCF-7
Sample Loading Amount：30 µg
Primary Antibody Dilution Factor：1:5000
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
Good
Review for anti-GAPDH antibody
Application：WB
Sample：A: mouse brain; B: rat brain
Sample Loading Amount：30 µg
Primary Antibody Dilution Factor：1:3000
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
Excellent
Review for anti-GAPDH antibody
Application：WB
Sample：HeLa cell lysate
Sample Loading Amount：30 µg
Primary Antibody Dilution Factor：1:500
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
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