ARG65714
anti-Vinculin antibody
anti-Vinculin antibody for Western blot and Human,Mouse,Rat
Cell Biology and Cellular Response antibody; Controls and Markers antibody; Signaling Transduction antibody; Loading Control antibody; Loading Control antibody for cytoplasmic fraction; High MW loading control antibody
1
Overview
| Product Description | Rabbit Polyclonal antibody recognizes Vinculin |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | Vinculin |
| Antigen Species | Human |
| Immunogen | Recombinant protein of Human Vinculin |
| Conjugation | Un-conjugated |
| Alternate Names | HEL114; Metavinculin; CMH15; Vinculin; CMD1W; MV; MVCL |
Application Instructions
| Predict Reactivity Note | Rat | ||||
|---|---|---|---|---|---|
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 50% Glycerol |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | VCL |
| Gene Full Name | vinculin |
| Background | Vinculin is a cytoskeletal protein associated with cell-cell and cell-matrix junctions, where it is thought to function as one of several interacting proteins involved in anchoring F-actin to the membrane. Defects in VCL are the cause of cardiomyopathy dilated type 1W. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Multiple alternatively spliced transcript variants have been found for this gene, but the biological validity of some variants has not been determined. [provided by RefSeq, Jul 2008] |
| Function | Actin filament (F-actin)-binding protein involved in cell-matrix adhesion and cell-cell adhesion. Regulates cell-surface E-cadherin expression and potentiates mechanosensing by the E-cadherin complex. May also play important roles in cell morphology and locomotion. [UniProt] |
| Highlight | Related Antibody Duos and Panels: ARG30258 Loading Controls for Whole Cell Lysate Antibody Panel ARG30262 PP2A alpha/beta and loading control Antibody Duo (PP2A, Vinculin) Related products: Vinculin antibodies; Vinculin Duos / Panels; Anti-Rabbit IgG secondary antibodies; |
| Research Area | Cell Biology and Cellular Response antibody; Controls and Markers antibody; Signaling Transduction antibody; Loading Control antibody; Loading Control antibody for cytoplasmic fraction; High MW loading control antibody |
| Calculated MW | 124 kDa |
| PTM | Phosphorylated; on serines, threonines and tyrosines. Phosphorylation on Tyr-1133 in activated platelets affects head-tail interactions and cell spreading but has no effect on actin binding nor on localization to focal adhesion plaques (By similarity). Acetylated; mainly by myristic acid but also by a small amount of palmitic acid. |
Images (2) Click the Picture to Zoom In
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ARG65714 anti-Vinculin antibody WB image
Western blot: 30 µg of Mouse heart, and HeLa cell lysate stained with ARG65714 anti-Vinculin antibody at 1:500 dilution.
Customer's Feedback
Excellent
Review for anti-Vinculin antibody
Application：WB
Sample：HeLa treated with TNFa at 0, 10, 20, and 30 min
Sample Loading Amount：30 µg
Primary Antibody Dilution Factor：1:500
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
