ARG80141
Human FGF basic ELISA Kit
Human FGF basic ELISA Kit for ELISA and Human
Cancer kit; Cell Biology and Cellular Response kit; Developmental Biology kit; Neuroscience kit; Signaling Transduction kit
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG80141-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG80141-002 | Standard (Lyophilized) | 3 X 4 ng/vial | 4°C |
| ARG80141-003 | Standard diluent buffer | 20 ml | 4°C |
| ARG80141-004 | Antibody conjugate concentrate | 400 µl | 4°C |
| ARG80141-005 | Antibody diluent buffer | 16 ml | 4°C |
| ARG80141-006 | HRP-Streptavidin concentrate | 400 µl | 4°C (Protect from light) |
| ARG80141-007 | HRP-Streptavidin diluent buffer | 16 ml | 4°C |
| ARG80141-008 | 20X Wash buffer | 50 ml | 4°C |
| ARG80141-009 | TMB substrate | 12ml | 4°C (Protect from light) |
| ARG80141-010 | STOP solution | 12ml | 4°C |
| ARG80141-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG80141 Human FGF basic ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human FGF basic in serum, plasma and cell culture supernatants. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Specificity | No significant cross-reactivity or interference with Human FGF-5,6,9,10,16,17,18,19,20,21,23, KGF； mouse FGF-acidic,FGF-9 |
| Target Name | FGF basic |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm |
| Sensitivity | 65 pg/ml |
| Sample Type | Serum, plasma and cell culture supernatants. |
| Standard Range | 125 - 8000 pg/ml |
| Sample Volume | 100 µl |
| Precision | CV: less than10% |
| Alternate Names | FGF-2; Fibroblast growth factor 2; bFGF; FGFB; Heparin-binding growth factor 2; BFGF; HBGF-2; Basic fibroblast growth factor |
Application Instructions
| Assay Time | 4 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | FGF2 |
| Gene Full Name | fibroblast growth factor 2 (basic) |
| Background | The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members bind heparin and possess broad mitogenic and angiogenic activities. This protein has been implicated in diverse biological processes, such as limb and nervous system development, wound healing, and tumor growth. The mRNA for this gene contains multiple polyadenylation sites, and is alternatively translated from non-AUG (CUG) and AUG initiation codons, resulting in five different isoforms with distinct properties. The CUG-initiated isoforms are localized in the nucleus and are responsible for the intracrine effect, whereas, the AUG-initiated form is mostly cytosolic and is responsible for the paracrine and autocrine effects of this FGF. [provided by RefSeq, Jul 2008] |
| Function | Plays an important role in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. Functions as potent mitogen in vitro. [UniProt] |
| Highlight | Related products: FGF basic antibodies; FGF basic ELISA Kits; FGF basic recombinant proteins; Related news: Vascular development is regulated by FGF-dependent metabolic control The role of HDGF in tumor angiogenesis New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| Research Area | Cancer kit; Cell Biology and Cellular Response kit; Developmental Biology kit; Neuroscience kit; Signaling Transduction kit |
| PTM | Phosphorylation at Tyr-215 regulates FGF2 unconventional secretion. Several N-termini starting at positions 94, 125, 126, 132, 143 and 162 have been identified by direct sequencing. |
Images (1) Click the Picture to Zoom In
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ARG80141 Human FGF basic ELISA Kit standard curve image
ARG80141 Human FGF basic ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG80141 Human FGF basic (bFGF) ELISA Kit User's manual |
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