ARG30151

Glucose uptake: Insulin Receptor Dependent Pathway Antibody Panel (GLUT4, AKT pS473, IRS1 pS636)

Datasheet

Publication8

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Price (USD)

1 kit

710.00

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Datasheet
Publication (8)

Component

Cat No	Component Name	Host clonality	Reactivity	Application	Package
ARG51720	anti-IRS1 phospho (Ser636) antibody	Rabbit pAb	Hu, Ms, Rat	IHC-P, WB	50 μl
ARG51558	anti-Akt phospho (Ser473) antibody	Rabbit pAb	Hu, Ms, Rat	ICC/IF, IHC-P, WB	50 μl
ARG65292	anti-GLUT4 antibody	Goat pAb	Hu, Ms, Rat	IHC-P, WB	50 μg
ARG65351	Goat anti-Rabbit IgG antibody (HRP)	Goat pAb	Rb	ELISA, IHC-P, WB	50 μl
ARG65352	Donkey anti-Goat IgG antibody (HRP)	Donkey pAb	Goat	ELISA, IHC, WB	50 μl

Overview

Product Description	Glucose is the primary energy source for most cells of the body. The study of glucose metabolism is central to cell proliferation, growth, survival, and most recently, tumor progression. Insulin stimulates glucose uptake from blood into skeletal muscle and adipose tissue through a signaling cascade mediated by the insulin receptor (IR). Insulin binding to the IR results in activation of the insulin receptor substrate (IRS) protein and subsequent signaling to the PI3K/Akt pathways, resulting in translocation of Glut4 vesicles, glucose uptake, cell proliferation, and survival. Otherwise, glucose uptake also can be stimulated by adrenoceptor. Upon ligand binding, b3-adrenoceptors result in increased levels of intracellular cAMP that lead to increased de novo synthesis of GLUT1 and mTORC2 phosphorylation. The newly produced GLUT1 is then translocated to the plasma membrane and helps the glucose uptake from blood. These two antibody PANELs can be used to identify the glucose uptake through insulin or adrenoceptors dependent pathway. Mihaylova MM, Shaw RJ (2011) Nat. Cell Biol. 13(9), 1016–23. Zemva J, Schubert M (2011) Curr Diabetes Rev 7(5), 356–66. Olsen, J. M. et al (2014) J. Cell Biol.207(3), 365-374.
Target Name	Glucose uptake: Insulin Receptor Dependent Pathway
Alternate Names	Glucose uptake: Insulin Receptor Dependent Pathway antibody; Akt phospho (Ser473) antibody; IRS1 phospho (Ser636) antibody; GLUT4 antibody

Properties

Storage Instruction	For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note	For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Gene Full Name	Antibody Panel for Glucose uptake: Insulin Receptor Dependent Pathway (GLUT4, AKT pS473, IRS1 pS636)
Research Area	Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Controls and Markers antibody; Developmental Biology antibody; Gene Regulation antibody; Immune System antibody; Metabolism antibody; Neuroscience antibody; Signaling Transduction antibody

Images (9) Click the Picture to Zoom In

ARG51558 anti-Akt phospho (Ser473) antibody WB image

Western blot: 30 µg of HeLa cells untreated or treated with EGF. Lysates stained with ARG51558 anti-Akt phospho (Ser473) antibody at 1:500 dilution.
ARG51558 anti-Akt phospho (Ser473) antibody WB image

Western Blot: extracts from 293 cells, treatedwith EGF or calf intestinal phosphatase (CIP), stained with anti-Akt (phospho Ser473) antibody ARG51558.
ARG51558 anti-Akt phospho (Ser473) antibody WB image

Western Blot: extracts from SK-BR-3 cells,treated with insulin and EGF, and pretreated with U0126and LY294002 cells stained with anti-Akt (phospho Ser473) antibody ARG51558.
ARG51720 anti-IRS-1 phospho (Ser636) antibody WB image

Western Blot: extracts from 3T3 cells untreated or treated with Insulin stained with anti-IRS-1 (phospho Ser636) antibody ARG51720.
ARG65292 anti-SLC2A4 / GLUT4 antibody WB image

Western Blot: Mouse Heart lysate (35 µg protein in RIPA buffer) stained with ARG65292 anti-SLC2A4 / GLUT4 antibody at 1 µg/ml dilution.
ARG65292 anti-GLUT4 antibody IHC-P image

Immunohistochemistry: Paraffin-embedded Human skeletal muscle tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG65292 anti-GLUT4 antibody at 3.75 µg/ml dilution followed by AP-staining.
ARG51558 anti-Akt phospho (Ser473) antibody IHC-P image

Immunohistochemistry: paraffin-embedded human breast carcinoma tissue stained with anti-Akt (phospho Ser473) antibody ARG51558 (left) or the same antibody preincubated with blocking peptide (right).
ARG51558 anti-Akt phospho (Ser473) antibody IHC-P image

Immunohistochemistry: paraffinembeddedhuman Lung carcinoma tissue stained with anti-Akt (phospho Ser473) antibody ARG51558.
ARG51720 anti-IRS-1 phospho (Ser636) antibody IHC-P image

Immunohistochemistry: paraffin-embedded human breast carcinoma tissue stained with anti-IRS-1 (phospho Ser636) antibody ARG51720 (left) or the same antibody preincubated with blocking peptide (right).

Specific References

Sika Deer Velvet Antler Peptide Exerts Neuroprotective Effect in a Parkinson's Disease Model via Regulating Oxidative Damage and Gut Microbiota

ARG51558: WB / Mouse

Ying Liu et al.
Pharmaceuticals (Basel)., (2024)

Downregulation of AKT/mTOR signaling pathway for Salmonella-mediated autophagy in human anaplastic thyroid cancer

ARG51558: IHC-P / Mouse

Li-Hsien Wu et al.
J Cancer, (2022)

Dopamine D2 receptor regulates cortical synaptic pruning in rodents

ARG51558: WB / Rat

Ya-Qiang Zhang et al.
Nat Commun., (2021)

1,3-dichloro-2-propanol induced lipid accumulation by blocking autophagy flux in HepG2 cells.

ARG51558: WB / Human

Jing Lu et al.
Toxicology., (2021)

Mild hypothermia protects rat cortical neurons against oxygen-glucose deprivation/reoxygenation injury via the PI3K/Akt pathway.

ARG51558: WB, ICC/IF / Rat

Zan Gao et al.
Neuroreport., (2021)

Pharmacological blockage of ICAM-1 improves angiotensin II-induced cardiac remodeling by inhibiting adhesion of LFA-1+ monocytes.

ARG51558: WB / Mouse

Lin Qiu-Yue et al.
Am J Physiol Heart Circ Physiol., (2019)

Protective effects of dioscin against fructose-induced renal damage via adjusting Sirt3-mediatedoxidative stress, fibrosis, lipid metabolism and inflammation.

ARG51558: WB / Rat

Qiao Yujie et al.
Toxicol Lett., (2017)

电针对控制性超排卵小鼠子宫内膜磷酸化胰岛素样生长因子-1受体表达及其信号转导通路的调控

ARG51558: WB / Mouse

朱书秀 et al.
Journal of Beijing University of Traditional Chinese Medicine., (2017)