ARG65292
anti-GLUT4 antibody
anti-GLUT4 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
Cancer antibody; Cell Biology and Cellular Response antibody; Developmental Biology antibody; Metabolism antibody; Signaling Transduction antibody; Glucose uptake: Insulin Receptor Dependent Pathway Study antibody
Overview
| Product Description | Goat Polyclonal antibody recognizes GLUT4 |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Predict Reactivity | Cow, Pig |
| Tested Application | IHC-P, WB |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | GLUT4 |
| Antigen Species | Human |
| Immunogen | C-TELEYLGPDEND |
| Conjugation | Un-conjugated |
| Alternate Names | Glucose transporter type 4, insulin-responsive; GLUT4; GLUT-4; Solute carrier family 2, facilitated glucose transporter member 4 |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|---|---|
| Application Note | WB: Recommend incubate at RT for 1h. IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Background | This gene is a member of the solute carrier family 2 (facilitated glucose transporter) family and encodes a protein that functions as an insulin-regulated facilitative glucose transporter. In the absence of insulin, this integral membrane protein is sequestered within the cells of muscle and adipose tissue. Within minutes of insulin stimulation, the protein moves to the cell surface and begins to transport glucose across the cell membrane. Mutations in this gene have been associated with noninsulin-dependent diabetes mellitus (NIDDM). [provided by RefSeq, Jul 2008] |
| Highlight | Related Antibody Duos and Panels: ARG30151 Glucose uptake: Insulin Receptor Dependent Pathway Antibody Panel (GLUT4, AKT pS473, IRS1 pS636) Related products: GLUT4 antibodies; GLUT4 Duos / Panels; Anti-Goat IgG secondary antibodies; |
| Research Area | Cancer antibody; Cell Biology and Cellular Response antibody; Developmental Biology antibody; Metabolism antibody; Signaling Transduction antibody; Glucose uptake: Insulin Receptor Dependent Pathway Study antibody |
| Calculated MW | 55 kDa |
| PTM | Sumoylated. |
Images (2) Click the Picture to Zoom In
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ARG65292 anti-GLUT4 antibody WB image
Western blot: Mouse Heart lysate (35 µg protein in RIPA buffer) stained with ARG65292 anti-GLUT4 antibody at 1 µg/ml dilution.
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ARG65292 anti-GLUT4 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human skeletal muscle tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG65292 anti-GLUT4 antibody at 3.75 µg/ml dilution followed by AP-staining.
