ARG30139
Cell Cycle Marker Antibody Panel (Cyclin B1, Cyclin E)
Cancer antibody; Cell Biology and Cellular Response antibody; Gene Regulation antibody; Immune System antibody
Publication3
Component
| Cat No | Component Name | Host clonality | Reactivity | Application | Package |
|---|---|---|---|---|---|
| ARG52927 | anti-Cyclin E1 antibody | Rabbit pAb | Hu | IHC-P | 250 μl |
| ARG62456 | anti-Cyclin B1 antibody [V152] | Mouse mAb | Hu | FACS, ICC/IF, IHC-Fr, IHC-P, WB | 250 μl |
| ARG51679 | anti-Histone H3 phospho (Ser10) antibody | Rabbit pAb | Hu, Ms, Rat, Cydippids | FACS, ICC/IF, IHC-P, WB | 50 μl |
| ARG65350 | Goat anti-Mouse IgG antibody (HRP) | Goat pAb | Ms | ELISA, IHC-P, WB | 50 μl |
| ARG65351 | Goat anti-Rabbit IgG antibody (HRP) | Goat pAb | Rb | ELISA, IHC-P, WB | 50 μl |
Overview
| Product Description | The cell cycle is controlled by numerous mechanisms ensuring correct cell division. Cell division consists of two consecutive processes, mainly characterized by DNA replication (S phase), proceeding after G1 interphase; and segregation of replicated chromosomes into two separate cells (M phase), following G2 phase. Cyclin E is upregulated during G1/S progression whereas Cyclin A and Cyclin B are expressed highly during G2/M progression. Phosphorylated Histone H3 is a distinct marker for Mitosis and often used in determining cells undergoing M-phase in a proliferating populations. Arigo’s Cell Cycle Phase Marker Panel provides a useful tool as markers for cells undergoing different phases in cell cycle. Vermeulen et al. (2003) Cell Prolif 36:131-149 Rahman et al. (2010) Cell Cycle 9: 22-27 Hans and Dimitrov (2001) Oncogene 24: 3021-3027 |
|---|---|
| Target Name | Cell Cycle Marker |
| Alternate Names | Cell Cycle Marker antibody; Histone H3 phospho (Ser10) antibody; Cyclin E1 antibody; Cyclin B1 antibody |
Properties
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
|---|---|
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Gene Full Name | Antibody Panel for Cell Cycle Marker (Cyclin B1, Cyclin E) |
|---|---|
| Research Area | Cancer antibody; Cell Biology and Cellular Response antibody; Gene Regulation antibody; Immune System antibody |
Images (8) Click the Picture to Zoom In
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ARG62456 anti-Cyclin B1 antibody [V152] WB image
Western blot: 20 µl of HeLa cell lysate stained with ARG62456 anti-Cyclin B1 antibody [V152] at 1 µg/ml dilution.
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ARG51679 anti-Histone H3 phospho (Ser10) antibody ICC/IF image
Immunofluorescence: Hydractinia stained with ARG51679 anti-Histone H3 phospho (Ser10) antibody.
From Gonzalo Quiroga-Artigas et al. Open Biol. (2022), doi: 10.1098/rsob.220120, Fig. 3.
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ARG51679 anti-Histone H3 phospho (Ser10) antibody ICC/IF image
Immunofluorescence: 100% Methanol fixed (RT, 10 min) HeLa cells stained with ARG51679 anti-Histone H3 phospho (Ser10) antibody at 1:100 dilution. Left: primary antibody (orange). Middle: DAPI (blue). Right: Merge.
Secondary antibody: ARG21917 Goat anti-Rabbit IgG antibody (TRITC).
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ARG51679 anti-Histone H3.1 phospho (Ser10) antibody WB image
Western Blot: extracts from SK-BR-3 cells, treated with insulin and EGF, and pretreated with U0126 and LY294002 cells stained with anti-Histone H3.1 (phospho Ser10) antibody ARG51679.
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ARG51679 anti-Histone H3.1 phospho (Ser10) antibody ICC/IF image
Immunofluorescence: methanol-fixed HeLa cells stained with anti-Histone H3.1 (phospho Ser10) antibody ARG51679.
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ARG51679 anti-Histone H3.1 phospho (Ser10) antibody IHC-P image
Immunohistochemistry: paraffin-embedded human breast carcinoma tissue stained with anti-Histone H3.1 (phospho Ser10) antibody ARG51679 (left) or the same antibody preincubated with blocking peptide (right).
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ARG51679 anti-Histone H3.1 phospho (Ser10) antibody ICC/IF image
Immunofluorescence: methanol-fixed HeLa cells stained with anti-Histone H3.1 (phospho Ser10) antibody ARG51679.
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ARG52927 anti-Cyclin E antibody IHC-P image
Immunohistochemistry: Human Breast Carcinoma stained with Cyclin E antibody (ARG52927)
Specific References
KDF1 Promoted Proliferation, Migration and Invasion of Lung Adenocarcinoma Cells through Activating STAT3 and AKT Pathway
ARG65350: WB /
GNL3 is an evolutionarily conserved stem cell gene influencing cell proliferation, animal growth and regeneration in the hydrozoan Hydractinia
ARG51679: ICC-IF / Hydractinia
Silencing of AURKA augments the antitumor efficacy of the AURKA inhibitor MLN8237 on neuroblastoma cells.
ARG51679: IHC-P / Human
