ARG62456

anti-Cyclin B1 antibody [V152]

anti-Cyclin B1 antibody [V152] for Flow cytometry,ICC/IF,IHC-Frozen sections,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human

Cancer antibody; Cell Biology and Cellular Response antibody; Gene Regulation antibody; Cell Cycle Study antibody

Overview

Product Description Mouse Monoclonal antibody [V152] recognizes Cyclin B1
Tested Reactivity Hu
Predict Reactivity Ms
Tested Application FACS, ICC/IF, IHC-Fr, IHC-P, WB
Specificity Cyclin B1 expression is restricted to a specific short period of the cell cycle with cyclin B1 expression detected earlier and peaking in concentration before cyclin B2 expression.
Host Mouse
Clonality Monoclonal
Clone V152
Isotype IgG1
Target Name Cyclin B1
Antigen Species Hamster
Immunogen His-tagged Hamster Cyclin B1 expressed in bacteria
Conjugation Un-conjugated
Alternate Names G2/mitotic-specific cyclin-B1; CCNB

Application Instructions

Application Suggestion
Tested Application Dilution
FACSAssay dependent
ICC/IFAssay dependent
IHC-Fr1 - 3 µg/ml
IHC-P1 - 3 µg/ml
WB1 - 3 µg/ml
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Protein G purified
Buffer PBS (pH 7.4) and 0.02% Sodium azide
Preservative 0.02% Sodium azide
Concentration 0.2 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 891 Human CCNB1

Swiss-port # P14635 Human G2/mitotic-specific cyclin-B1

Gene Symbol Ccnb1
Gene Full Name cyclin B1
Background The protein encoded by this gene is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase. The different transcripts result from the use of alternate transcription initiation sites. [provided by RefSeq, Jul 2008]
Highlight Related Antibody Duos and Panels:
ARG30139 Cell Cycle Marker Antibody Panel (Cyclin B1, Cyclin E)
Related products:
Cyclin B1 antibodies; Cyclin B1 Duos / Panels; Anti-Mouse IgG secondary antibodies;
Research Area Cancer antibody; Cell Biology and Cellular Response antibody; Gene Regulation antibody; Cell Cycle Study antibody
Calculated MW 48 kDa
PTM Ubiquitinated by the SCF(NIPA) complex during interphase, leading to its destruction. Not ubiquitinated during G2/M phases.
Phosphorylated by PLK1 at Ser-133 on centrosomes during prophase: phosphorylation by PLK1 does not cause nuclear import. Phosphorylation at Ser-147 was also reported to be mediated by PLK1 but Ser-133 seems to be the primary phosphorylation site.

Images (1) Click the Picture to Zoom In

  • ARG62456 anti-Cyclin B1 antibody [V152] WB image

    Western blot: 20 µl of HeLa cell lysate stained with ARG62456 anti-Cyclin B1 antibody [V152] at 1 µg/ml dilution.

Clone References

Casein kinase 1δ-dependent Wee1 protein degradation.

WB / Human

Penas C et al.
J Biol Chem.,  (2014)

publication_link

 

hr_line

Dual-mode regulation of the APC/C by CDK1 and MAPK controls meiosis I progression and fidelity.

WB / 

Nabti I et al.
J Cell Biol.,  (2014)

publication_link

 

hr_line

Human parvovirus B19 infection causes cell cycle arrest of human erythroid progenitors at late S phase that favors viral DNA replication.

Luo Y et al.
J Virol.,  (2013)

publication_link

 

hr_line

Tumor dynamics in response to antiangiogenic therapy with oral metronomic topotecan and pazopanib in neuroblastoma xenografts.

IHC-P / Mouse

Kumar S et al.
Transl Oncol.,  (2013)

publication_link

 

hr_line

The role of RING box protein 1 in mouse oocyte meiotic maturation.

Zhou L et al.
PLoS One.,  (2013)

publication_link

 

hr_line

APC(FZR1) prevents nondisjunction in mouse oocytes by controlling meiotic spindle assembly timing.

WB / 

Holt JE et al.
Mol Biol Cell.,  (2012)

publication_link

 

hr_line

Plk1 negatively regulates PRC1 to prevent premature midzone formation before cytokinesis.

ICC/IF / 

Hu CK et al.
Mol Biol Cell.,  (2012)

publication_link

 

hr_line

Cellular distributions of molecules with altered expression specific to thyroid proliferative lesions developing in a rat thyroid carcinogenesis model.

IHC / Rat

Woo GH et al.
Cancer Sci.,  (2009)

publication_link

 

hr_line

Antigenic profiling of glioma cells to generate allogeneic vaccines or dendritic cell-based therapeutics.

FACS / Human

Zhang JG et al.
Clin Cancer Res.,  (2007)

publication_link

 

hr_line

Citron kinase is a cell cycle-dependent, nuclear protein required for G2/M transition of hepatocytes.

WB / Rat

Liu H et al.
J Biol Chem.,  (2003)

publication_link

 

hr_line

Kinetics of endomitosis in primary murine megakaryocytes.

FACS / Mouse

Carow CE et al.
J Cell Physiol.,  (2001)

publication_link

 

hr_line