ARG51698
anti-p21 phospho (Thr145) antibody
anti-p21 phospho (Thr145) antibody for IHC-Formalin-fixed paraffin-embedded sections and Human
Cancer antibody; Cell Biology and Cellular Response antibody; Gene Regulation antibody
Overview
| Product Description | Rabbit Polyclonal antibody recognizes p21 phospho (Thr145) |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | IHC-P |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | p21 |
| Antigen Species | Human |
| Immunogen | Peptide sequence around phosphorylation site of Thr145 (R-Q-T(p)-S-M) derived from Human p21. |
| Conjugation | Un-conjugated |
| Alternate Names | Melanoma differentiation-associated protein 6; WAF1; CIP1; CDKN1; CAP20; MDA-6; SDI1; CDK-interacting protein 1; P21; p21CIP1; p21; Cyclin-dependent kinase inhibitor 1 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Antibodies were produced by immunizing rabbits with KLH-conjugated synthetic phosphopeptide. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. In addition, non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide. |
| Buffer | PBS (without Mg2+ and Ca2+, pH 7.4), 150mM NaCl, 0.02% Sodium azide and 50% Glycerol. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 50% Glycerol |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P38936 Human Cyclin-dependent kinase inhibitor 1 |
|---|---|
| Gene Symbol | CDKN1A |
| Gene Full Name | cyclin-dependent kinase inhibitor 1A (p21, Cip1) |
| Background | May be the important intermediate by which p53 mediates its role as an inhibitor of cellular proliferation in response to DNA damage. Binds to and inhibits cyclin-dependent kinase activity, preventing phosphorylation of critical cyclin-dependent kinase substrates and blocking cell cycle progression. |
| Function | May be the important intermediate by which p53/TP53 mediates its role as an inhibitor of cellular proliferation in response to DNA damage. Binds to and inhibits cyclin-dependent kinase activity, preventing phosphorylation of critical cyclin-dependent kinase substrates and blocking cell cycle progression. Functions in the nuclear localization and assembly of cyclin D-CDK4 complex and promotes its kinase activity towards RB1. At higher stoichiometric ratios, inhibits the kinase activity of the cyclin D-CDK4 complex. [UniProt] |
| Highlight | Related products: p21 antibodies; p21 Duos / Panels; Anti-Rabbit IgG secondary antibodies; Related news: Senescence Marker Antibody Panel is launched |
| Research Area | Cancer antibody; Cell Biology and Cellular Response antibody; Gene Regulation antibody |
| Calculated MW | 18 kDa |
| PTM | Phosphorylation of Thr-145 by Akt or of Ser-146 by PKC impairs binding to PCNA. Phosphorylation at Ser-114 by GSK3-beta enhances ubiquitination by the DCX(DTL) complex. Phosphorylation of Thr-145 by PIM2 enhances CDKN1A stability and inhibits cell proliferation. Phosphorylation of Thr-145 by PIM1 results in the relocation of CDKN1A to the cytoplasm and enhanced CDKN1A protein stability. UV radiation-induced phosphorylation at Thr-80 by LKB1 and at Ser-146 by NUAK1 leads to its degradation. Ubiquitinated by MKRN1; leading to polyubiquitination and 26S proteasome-dependent degradation. Ubiquitinated by the DCX(DTL) complex, also named CRL4(CDT2) complex, leading to its degradation during S phase or following UV irradiation. Ubiquitination by the DCX(DTL) complex is essential to control replication licensing and is PCNA-dependent: interacts with PCNA via its PIP-box, while the presence of the containing the 'K+4' motif in the PIP box, recruit the DCX(DTL) complex, leading to its degradation. Ubiquitination at Ser-2 leads to degradation by the proteasome pathway. Ubiquitinated by RNF114; leading to proteasomal degradation. Acetylation leads to protein stability. Acetylated in vitro on Lys-141, Lys-154, Lys-161 and Lys-163. Deacetylation by HDAC1 is prevented by competitive binding of C10orf90/FATS to HDAC1 (By similarity). |
Images (1) Click the Picture to Zoom In
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ARG51698 anti-p21 phospho (Thr145) antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human breast carcinoma tissue stained with ARG51698 anti-p21 phospho (Thr145) antibody (left) or the same antibody preincubated with blocking peptide (right).
