ARG53372

anti-iNOS antibody [SP126]

Datasheet

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100 μl

379.00

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Datasheet
Publication

Overview

Product Description	Rabbit Monoclonal antibody [SP126] recognizes iNOS
Tested Reactivity	Hu
Predict Reactivity	Ms, Rat
Tested Application	IHC-P
Host	Rabbit
Clonality	Monoclonal
Clone	SP126
Isotype	IgG
Target Name	iNOS
Antigen Species	Human
Immunogen	Synthetic peptide near the C-terminus of mouse iNOS protein.
Conjugation	Un-conjugated
Alternate Names	HEP-NOS; Inducible NO synthase; INOS; Nitric oxide synthase, inducible; iNOS; Hepatocyte NOS; NOS2A; Peptidyl-cysteine S-nitrosylase NOS2; Inducible NOS; NOS; NOS type II; EC 1.14.13.39

Application Instructions

Application Suggestion

Tested Application	Dilution
IHC-P	1:100

Application Note

IHC-P: Antigen Retrieval: Boil tissue section in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.
Incubation Time: 10 min at RT.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Positive Control

Lung

Properties

Form	Liquid
Purification	Purified by protein A/G
Buffer	PBS (pH 7.6), 1% BSA and <0.1% Sodium azide
Preservative	<0.1% Sodium azide
Stabilizer	1% BSA
Storage Instruction	For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note	For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links	GeneID: 4843 Human NOS2 Swiss-port # P35228 Human Nitric oxide synthase, inducible
Gene Symbol	NOS2
Gene Full Name	nitric oxide synthase 2, inducible
Background	iNOS: Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. Nitric oxide synthase is expressed in liver and is inducible by a combination of lipopolysaccharide and certain cytokines. Three related pseudogenes are located within the Smith-Magenis syndrome region on chromosome 17. [provided by RefSeq, Jul 2008]
Function	iNOS produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body (PubMed:7531687, PubMed:7544004). In macrophages, NO mediates tumoricidal and bactericidal actions. Also has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such PTGS2/COX2. As component of the iNOS-S100A8/9 transnitrosylase complex involved in the selective inflammatory stimulus-dependent S-nitrosylation of GAPDH on 'Cys-247' implicated in regulation of the GAIT complex activity and probably multiple targets including ANXA5, EZR, MSN and VIM (PubMed:25417112). Involved in inflammation, enhances the synthesis of proinflammatory mediators such as IL6 and IL8 (PubMed:19688109). [UniProt]
Cellular Localization	Cytoplasm
Highlight	Related products: iNOS antibodies; iNOS ELISA Kits; iNOS Duos / Panels; Anti-Rabbit IgG secondary antibodies; Related news: Tumor-Infiltrating Lymphocytes (TILs) New antibody panels and duos for Tumor immune microenvironment Exploring Antiviral Immune Response Anti-SerpinB9 therapy, a new strategy for cancer therapy RIP1 activation and pathogenesis of NASH
Research Area	Inflammation Study antibody; M1/M2/TAM Marker antibody; Macrophage Marker antibody; M1 macrophage Marker antibody
Calculated MW	131 kDa

Images (2) Click the Picture to Zoom In

ARG53372 anti-Nitric Oxide Synthase-Inducible (iNOS) antibody [SP126] IHC-P image

Immunohistochemistry: Human Lung stained with Nitric Oxide Synthase-Inducible (iNOS) antibody [SP126] (ARG53372)
ARG53372 anti-Nitric Oxide Synthase-Inducible (iNOS) antibody [SP126] IHC-P image

Immunohistochemistry: Human Lung stained with Nitric Oxide Synthase-Inducible (iNOS) antibody [SP126] (ARG53372)