ARG58784
anti-hemoglobin beta + delta antibody
anti-hemoglobin beta + delta antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
Overview
| Product Description | Rabbit Polyclonal antibody recognizes hemoglobin beta + delta |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | hemoglobin beta + delta |
| Antigen Species | Human |
| Immunogen | Recombinant protein corresponding to V2-H147 of Human hemoglobin delta. |
| Conjugation | Un-conjugated |
| Alternate Names | Delta-globin; Hemoglobin delta chain; Hemoglobin subunit delta |
Application Instructions
| Application Suggestion |
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| Application Note | IHC-P: Antigen Retrieval: Heat mediated was performed in Citrate buffer (pH 6.0) for 20 min. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | 0.9% NaCl, 0.2% Na2HPO4, 0.05% Sodium azide and 5% BSA. |
| Preservative | 0.05% Sodium azide |
| Stabilizer | 5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | HBD |
| Gene Full Name | hemoglobin, delta |
| Background | The delta (HBD) and beta (HBB) genes are normally expressed in the adult: two alpha chains plus two beta chains constitute HbA, which in normal adult life comprises about 97% of the total hemoglobin. Two alpha chains plus two delta chains constitute HbA-2, which with HbF comprises the remaining 3% of adult hemoglobin. Five beta-like globin genes are found within a 45 kb cluster on chromosome 11 in the following order: 5'-epsilon--Ggamma--Agamma--delta--beta-3'. Mutations in the delta-globin gene are associated with beta-thalassemia. [provided by RefSeq, Jul 2008] |
| Function | Involved in oxygen transport from the lung to the various peripheral tissues. [UniProt] |
| Calculated MW | 16 kDa |
Images (8) Click the Picture to Zoom In
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ARG58784 anti-hemoglobin beta + delta antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human intestinal cancer tissue. Antigen Retrieval: Heat mediated was performed in Citrate buffer (pH 6.0, epitope retrieval solution) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG58784 anti-hemoglobin beta + delta antibody at 1 µg/ml, overnight at 4°C.
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ARG58784 anti-hemoglobin beta + delta antibody WB image
Western blot: 50 µg of samples under reducing conditions. Human placenta, Rat spleen, Mouse spleen lysates stained with ARG58784 anti-hemoglobin beta + delta antibody at 0.5 µg/ml, overnight at 4°C.
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ARG58784 anti-hemoglobin beta + delta antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human lung cancer tissue. Antigen Retrieval: Heat mediated was performed in Citrate buffer (pH 6.0, epitope retrieval solution) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG58784 anti-hemoglobin beta + delta antibody at 1 µg/ml, overnight at 4°C.
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ARG58784 anti-hemoglobin beta + delta antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human mammary cancer tissue. Antigen Retrieval: Heat mediated was performed in Citrate buffer (pH 6.0, epitope retrieval solution) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG58784 anti-hemoglobin beta + delta antibody at 1 µg/ml, overnight at 4°C.
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ARG58784 anti-hemoglobin beta + delta antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human placenta tissue. Antigen Retrieval: Heat mediated was performed in Citrate buffer (pH 6.0, epitope retrieval solution) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG58784 anti-hemoglobin beta + delta antibody at 1 µg/ml, overnight at 4°C.
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ARG58784 anti-hemoglobin beta + delta antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Mouse liver tissue. Antigen Retrieval: Heat mediated was performed in Citrate buffer (pH 6.0, epitope retrieval solution) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG58784 anti-hemoglobin beta + delta antibody at 1 µg/ml, overnight at 4°C.
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ARG58784 anti-hemoglobin beta + delta antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Rat liver tissue. Antigen Retrieval: Heat mediated was performed in Citrate buffer (pH 6.0, epitope retrieval solution) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG58784 anti-hemoglobin beta + delta antibody at 1 µg/ml, overnight at 4°C.
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ARG58784 anti-hemoglobin beta + delta antibody WB image
Western blot: 50 µg of samples under reducing conditions. Human placenta lysate stained with ARG58784 anti-hemoglobin beta + delta antibody at 0.5 µg/ml, overnight at 4°C.
