ARG42137
anti-eEF1E1 / AIMP3 antibody
anti-eEF1E1 / AIMP3 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Pig
Overview
| Product Description | Goat Polyclonal antibody recognizes eEF1E1 / AIMP3 |
|---|---|
| Tested Reactivity | Hu, Pig |
| Predict Reactivity | Cow, Dog |
| Tested Application | IHC-P, WB |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | eEF1E1 / AIMP3 |
| Antigen Species | Human |
| Immunogen | Synthetic peptide around the internal region of Human eEF1E1 / AIMP3. (C-SKGNKYSAQGERQ) (NP_004271.1; NP_001129122.1) |
| Conjugation | Un-conjugated |
| Alternate Names | Eukaryotic translation elongation factor 1 epsilon-1; Elongation factor p18; AIMP3; P18; Multisynthase complex auxiliary component p18; Aminoacyl tRNA synthetase complex-interacting multifunctional protein 3 |
Application Instructions
| Application Suggestion |
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| Application Note | WB: Recommend incubate at RT for 1h. IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| Positive Control | K562 and Pig testis | ||||||
| Observed Size | ~ 20 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Ammonium sulphate precipitation followed by affinity purification with immunogen. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # O43324 Human Eukaryotic translation elongation factor 1 epsilon-1 |
|---|---|
| Gene Symbol | EEF1E1 |
| Gene Full Name | eukaryotic translation elongation factor 1 epsilon 1 |
| Background | This gene encodes a multifunctional protein that localizes to both the cytoplasm and nucleus. In the cytoplasm, the encoded protein is an auxiliary component of the macromolecular aminoacyl-tRNA synthase complex. However, its mouse homolog has been shown to translocate to the nucleus in response to DNA damage, and it plays a positive role in ATM/ATR-mediated p53 activation. Alternative splicing results in multiple transcript variants. Read-through transcription also exists between this gene and the neighboring downstream MUTED (muted homolog) gene. An EEF1E1-related pseudogene has been identified on chromosome 2. [provided by RefSeq, Dec 2010] |
| Function | Positive modulator of ATM response to DNA damage. [UniProt] |
| Cellular Localization | Cytoplasm. Cytoplasm, cytosol. Nucleus. Note=Cytoplasmic under growth arrest conditions. Translocated into the nucleus when growth resumes (S phase) and following DNA damage. [UniProt] |
| Calculated MW | 20 kDa |
Images (3) Click the Picture to Zoom In
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ARG42137 anti-eEF1E1 / AIMP3 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human pancreas tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG42137 anti-eEF1E1 / AIMP3 antibody at 5 µg/ml dilution followed by AP-staining.
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ARG42137 anti-eEF1E1 / AIMP3 antibody WB image
Western blot: 35 µg of K562 cell lysate (in RIPA buffer) stained with ARG42137 anti-eEF1E1 / AIMP3 antibody at 0.1 µg/ml dilution and incubated at RT for 1 hour.
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ARG42137 anti-eEF1E1 / AIMP3 antibody WB image
Western blot: 35 µg of Pig testis lysate (in RIPA buffer) stained with ARG42137 anti-eEF1E1 / AIMP3 antibody at 0.3 µg/ml dilution and incubated at RT for 1 hour.
