ARG40774

anti-dsDNA antibody [AE-2]

anti-dsDNA antibody [AE-2] for Flow cytometry,IHC-Formalin-fixed paraffin-embedded sections and Human

Overview

Product Description Mouse Monoclonal antibody [AE-2] recognizes dsDNA
Tested Reactivity Hu
Tested Application FACS, IHC-P
Specificity This antibody recognizes double-stranded deoxyribonucleic acid (dsDNA), but may demonstrate some cross-reactivity with single-stranded deoxyribonucleic acid (ssDNA).
Host Mouse
Clonality Monoclonal
Clone AE-2
Isotype IgG3, kappa
Target Name dsDNA
Antigen Species Human
Immunogen Nuclei of Burkitt's cells.
Conjugation Un-conjugated

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1 - 2 µg/10^6 cells
IHC-P1 - 2 µg/ml
Application Note IHC-P: Antigen Retrieval: Boil tissue section in 10 mM Citrate buffer (pH 6.0) for 10-20 min, followed by cooling at RT.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purification with Protein G.
Buffer PBS, 0.05% Sodium azide and 0.1 mg/ml BSA.
Preservative 0.05% Sodium azide
Stabilizer 0.1 mg/ml BSA
Concentration 0.2 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Images (3) Click the Picture to Zoom In

  • ARG40774 anti-dsDNA antibody [AE-2] FACS image

    Flow Cytometry: Fixed and permeabilized Jurkat cells stained with ARG40774 anti-dsDNA antibody [AE-2] (blue) or isotype control (red).

  • ARG40774 anti-dsDNA antibody [AE-2] IHC-P image

    Immunohistochemistry: Formalin-fixed and paraffin-embedded Human colon carcinoma stained with ARG40774 anti-dsDNA antibody [AE-2]. Antigen Retrieval: Boil tissue section in 10 mM Citrate buffer (pH 6.0) for 10-20 min followed by cooling at RT.

  • ARG40774 anti-dsDNA antibody [AE-2] IHC-P image

    Immunohistochemistry: Formalin-fixed and paraffin-embedded Human tonsil stained with ARG40774 anti-dsDNA antibody [AE-2]. Antigen Retrieval: Boil tissue section in 10 mM Citrate buffer (pH 6.0) for 10-20 min followed by cooling at RT.