ARG63621

Name: anti-alpha smooth muscle Actin antibody
Brand: arigo Biolaboratories
SKU: ARG63621
Price: 189 USD
Availability: InStock
Rating: 4.0 (1 reviews)

anti-alpha smooth muscle Actin antibody

Datasheet

Publication2

Ordering Information

Package

Price (USD)

20 μg

189.00

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Overview

Product Description	Goat Polyclonal antibody recognizes alpha smooth muscle Actin
Tested Reactivity	Hu, Ms
Predict Reactivity	Dog, Rat
Tested Application	ICC/IF, IHC-Fr, IHC-P, WB
Specificity	Variants NP_001604.1 and NP_001135417.1 encode the same protein.
Host	Goat
Clonality	Polyclonal
Isotype	IgG
Target Name	alpha smooth muscle Actin
Antigen Species	Human
Immunogen	EEEDSTALVC
Conjugation	Un-conjugated
Alternate Names	Cell growth-inhibiting gene 46 protein; MYMY5; ACTSA; Alpha-actin-2; Actin, aortic smooth muscle; AAT6

Application Instructions

Application Suggestion

Tested Application	Dilution
ICC/IF	Assay - dependent
IHC-Fr	Assay - dependent
IHC-P	Assay - dependent
WB	0.1 - 1 µg/ml

Application Note

WB: Recommend incubate at RT for 1h.
IHC-P: Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0).
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form	Liquid
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Buffer	Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA
Preservative	0.02% Sodium azide
Stabilizer	0.5% BSA
Concentration	0.5 mg/ml
Storage Instruction	For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note	For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links	GeneID: 11475 Mouse ACTA2 GeneID: 59 Human ACTA2 Swiss-port # P62736 Human Actin, aortic smooth muscle Swiss-port # P62737 Mouse Actin, aortic smooth muscle
Background	The protein encoded by this gene belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actins being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. This actin is an alpha actin that is found in skeletal muscle. Defects in this gene cause aortic aneurysm familial thoracic type 6. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Nov 2008]
Highlight	Related Antibody Duos and Panels: ARG30051 Smooth Muscle Marker Antibody Duo (Desmin, SMA) ARG30328 Angiogenesis Antibody Panel Related products: alpha smooth muscle Actin antibodies; alpha smooth muscle Actin Duos / Panels; Anti-Goat IgG secondary antibodies; Related news: New antibody panels for Myofibroblasts and CAFs Besides tumor suppression, what’s p53 busy for during embryogenesis? New antibody panels and duos for Tumor immune microenvironment Anti-SerpinB9 therapy, a new strategy for cancer therapy
Research Area	Cell Biology and Cellular Response antibody; Controls and Markers antibody; Signaling Transduction antibody; Cancer-associated fibroblast antibody; Smooth Muscle Marker antibody; CAF Marker antibody; Mural cell Marker antibody; Myofibroblast Marker antibody; Angiogenesis Study antibody; Microvascular Density Study antibody
Calculated MW	42 kDa
PTM	Oxidation of Met-46 and Met-49 by MICALs (MICAL1, MICAL2 or MICAL3) to form methionine sulfoxide promotes actin filament depolymerization. MICAL1 and MICAL2 produce the (R)-S-oxide form. The (R)-S-oxide form is reverted by MSRB1 and MSRB2, which promote actin repolymerization (By similarity). Monomethylation at Lys-86 (K84me1) regulates actin-myosin interaction and actomyosin-dependent processes. Demethylation by ALKBH4 is required for maintaining actomyosin dynamics supporting normal cleavage furrow ingression during cytokinesis and cell migration. (Microbial infection) Monomeric actin is cross-linked by V.cholerae toxins RtxA and VgrG1 in case of infection: bacterial toxins mediate the cross-link between Lys-52 of one monomer and Glu-272 of another actin monomer, resulting in formation of highly toxic actin oligomers that cause cell rounding (PubMed:19015515). The toxin can be highly efficient at very low concentrations by acting on formin homology family proteins: toxic actin oligomers bind with high affinity to formins and adversely affect both nucleation and elongation abilities of formins, causing their potent inhibition in both profilin-dependent and independent manners (PubMed:26228148).

Images (3) Click the Picture to Zoom In

ARG63621 anti-alpha smooth muscle Actin antibody WB image

Western blot: 30 µg of MEF cell lysate stained with ARG63621 anti-alpha smooth muscle Actin antibody at 1:300 dilution.
ARG63621 anti-alpha smooth muscle Actin antibody ICC/IF image

Immunofluorescence: Paraformaldehyde fixed HepG2 cells permeabilized with 0.15% Triton. Cells were stained with ARG63621 anti-alpha smooth muscle Actin antibody (green) at 10 µg/ml dilution for 1 hour. DAPI (blue) for nuclear staining. Negative control: Unimmunized goat IgG (green) at 10 µg/ml dilution.
ARG63621 anti-alpha smooth muscle Actin antibody IHC-P image

Immunohistochemistry: Paraffin-embedded Human lung tissue. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0). The tissue section was stained with ARG63621 anti-alpha smooth muscle Actin antibody at 3.5 µg/ml dilution followed by HRP-staining.