ARG10186
anti-alpha Fetoprotein antibody [AFP-Y1]
anti-alpha Fetoprotein antibody [AFP-Y1] for ELISA,Western blot and Human
Cancer antibody; Cell Biology and Cellular Response antibody; Developmental Biology antibody
Overview
| Product Description | Mouse Monoclonal antibody [AFP-Y1] recognizes Human AFP |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA, WB |
| Specificity | No detectable cross-reaction with human normal serum proteins. |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | AFP-Y1 |
| Isotype | IgG2b |
| Target Name | alpha Fetoprotein |
| Antigen Species | Human |
| Immunogen | Human AFP, which was isolated from amniotic fluid and purified by affinity chromatography. |
| Conjugation | Un-conjugated |
| Alternate Names | Alpha-fetoprotein; HPAFP; FETA; Alpha-fetoglobulin; AFPD; Alpha-1-fetoprotein |
Application Instructions
| Application Note | ELISA: This antibody can be used as a capture antibody in sandwich immunoassay for human AFP detection. Western blot: The antibody identifies 68kD AFP on western blot. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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Properties
| Form | Liquid |
|---|---|
| Purification | Protein G affinity purified |
| Buffer | 0.01M PBS (pH 7.0) |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | AFP |
| Gene Full Name | alpha-fetoprotein |
| Background | Alpha fetoprotein (AFP) is a 70kD glycoprotein which is produced primarily by liver cells in foetuses. It exists in significant amount in foetal plasma and decreases rapidly after birth. Moderate amount of AFP exists in serum of pregnant women. Monitoring mother’s serum AFP level in second trimester pregnancy can help assessing birth defects such as Down syndrome, neural tube defect and anencephaly. Elevated AFP level in men, children and non-pregnant women is associated with neoplastic conditions in lever, testicle or ovary. |
| Function | Binds copper, nickel, and fatty acids as well as, and bilirubin less well than, serum albumin. Only a small percentage (less than 2%) of the human AFP shows estrogen-binding properties. [UniProt] |
| Highlight | Related Antibody Duos and Panels: ARG30065 Alpha Fetoprotein ELISA Antibody Duo Related products: alpha Fetoprotein antibodies; alpha Fetoprotein ELISA Kits; alpha Fetoprotein Duos / Panels; Anti-Mouse IgG secondary antibodies; Related news: Stem cell and the regenerative medicine: Ready for the patients |
| Research Area | Cancer antibody; Cell Biology and Cellular Response antibody; Developmental Biology antibody |
| Calculated MW | 69 kDa |
| PTM | Independent studies suggest heterogeneity of the N-terminal sequence of the mature protein and of the cleavage site of the signal sequence. Sulfated. |
Images (1) Click the Picture to Zoom In
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ARG10186 anti-alpha Fetoprotein antibody [AFP-Y1] WB image
Western blot: 20 µl of HepG2 and MDA-MB-231 cell lysates stained with ARG10186 anti-alpha Fetoprotein antibody [AFP-Y1] at 1:500 dilution.
