ARG62669
anti-alpha Fetoprotein antibody [AFP-01]
anti-alpha Fetoprotein antibody [AFP-01] for ELISA,ICC/IF,Immunoprecipitation,Western blot and Human
Cancer antibody; Cell Biology and Cellular Response antibody; Developmental Biology antibody
Overview
| Product Description | Mouse Monoclonal antibody [AFP-01] recognizes alpha-Fetoprotein |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA, ICC/IF, IP, WB |
| Specificity | The clone AFP-01 reacts with human alpha-Fetoprotein (AFP), a 70 kDa oncofetal antigen. AFP is a major fetal plasma protein, but is not present in healthy adult tissues. Elevated AFP concentrations in adult plasma may be an early marker of hepatocellular carcinoma or teratoblastoma, while high concentrations in amniotic fluid may indicate severe congenital defects of a fetus. |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | AFP-01 |
| Isotype | IgG1 |
| Target Name | alpha Fetoprotein |
| Immunogen | purified alpha-Fetoprotein |
| Conjugation | Un-conjugated |
| Alternate Names | Alpha-fetoprotein; HPAFP; FETA; Alpha-fetoglobulin; AFPD; Alpha-1-fetoprotein |
Application Instructions
| Application Suggestion |
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| Application Note | IP: Interaction of the antibody AFP-01 with AFP is dependent on the presence of calcium ions (strongly inhibited by chelating agents). Such characteristics of the antibody can be exploited for immunoaffinity purification of APF under mild elution conditions. ELISA: The antibody AFP-01 has been tested as the detection antibody in a sandwich ELISA for analysis of human alpha-Fetoprotein in combination with antibody AFP-11. WB: Sample preparation: Resuspend approx. 50 mil. cells in 1 ml cold Lysis buffer (1% laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Protease inhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysate with non-reducing/reducing Laemmli SDS-PAGE sample buffer. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| Positive Control | WB: HepG2 |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from ascites by precipitation methods. |
| Purity | > 95% (by SDS-PAGE) |
| Buffer | PBS (pH 7.4) and 15 mM Sodium azide |
| Preservative | 15 mM Sodium azide |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | AFP |
| Gene Full Name | alpha-fetoprotein |
| Background | This gene encodes alpha-fetoprotein, a major plasma protein produced by the yolk sac and the liver during fetal life. Alpha-fetoprotein expression in adults is often associated with hepatoma or teratoma. However, hereditary persistance of alpha-fetoprotein may also be found in individuals with no obvious pathology. The protein is thought to be the fetal counterpart of serum albumin, and the alpha-fetoprotein and albumin genes are present in tandem in the same transcriptional orientation on chromosome 4. Alpha-fetoprotein is found in monomeric as well as dimeric and trimeric forms, and binds copper, nickel, fatty acids and bilirubin. The level of alpha-fetoprotein in amniotic fluid is used to measure renal loss of protein to screen for spina bifida and anencephaly. [provided by RefSeq, Jul 2008] |
| Function | Binds copper, nickel, and fatty acids as well as, and bilirubin less well than, serum albumin. Only a small percentage (less than 2%) of the human AFP shows estrogen-binding properties. [UniProt] |
| Highlight | Related products: alpha Fetoprotein antibodies; alpha Fetoprotein ELISA Kits; alpha Fetoprotein Duos / Panels; Anti-Mouse IgG secondary antibodies; Related news: Stem cell and the regenerative medicine: Ready for the patients |
| Research Area | Cancer antibody; Cell Biology and Cellular Response antibody; Developmental Biology antibody |
| Calculated MW | 69 kDa |
| PTM | Independent studies suggest heterogeneity of the N-terminal sequence of the mature protein and of the cleavage site of the signal sequence. Sulfated. |
Images (1) Click the Picture to Zoom In
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ARG62669 anti-alpha Fetoprotein antibody [AFP-01] WB image
Western blot: Hep G2 cell lysate stained with ARG62669 anti-alpha Fetoprotein antibody [AFP-01].
Lane 1: immunostaining with anti-human alpha-Fetoprotein under reducing conditions. Lane 2: immunostaining with anti-human alpha-Fetoprotein under non-reducing conditions.
Clone References
ROCK inhibitor is not required for embryoid body formation from singularized human embryonic stem cells.
ICC/IF / Human
Scalable passaging of adherent human pluripotent stem cells.
ICC/IF / Human
Global screening of human cord blood proteomes for biomarkers of toxic exposure and effect.
WB / Human
Regulation of alpha-fetoprotein by nuclear factor-kappaB protects hepatocytes from tumor necrosis factor-alpha cytotoxicity during fetal liver development and hepatic oncogenesis.
Monoclonal antibodies against human alpha-fetoprotein. Exploitation of an unusual calcium-dependent interaction with the antigen for analytical and preparative purposes.
